Serial Dilution

If 1 mL sample is put into tube #1 that has 24 ml diluent, what is the tube dilution? What is the final dilution?

• Tube 1/25

• Final 1/50

• Tube 1/50

• Final 1/25

If 1 mL sample form tube #1 with a tube dilution of 1/25 is placed in tube #2 with 24 mL of diluent, what is the tube dilution and what is the final dilution of tube #2?

• tube dilution 1/50

• tube dilution 1/25

• final dilution 1/625

• final dilution 1/50

If the concentration of tube 1 is 150 ng/ml (tube 1 is now the starting stock solution) and 1 ml of sample is moved each time into 24 ml of diluent to complete 3 dilutions from the first tube, what is the concentration in tube #4 or the third dilution?

• 150 ng/ml

• 6 ng/ml

• 0.24 ng/ml

• 0.0096 ng/ml

If 20 uL sample is put into tube #1 with 100 uL diluent, what is the tube dilution and final dilution of tube #1?

• tube dilution 20/100 or 1/5

• tube dilution 20/120 or 1/6

• final dilution 20/120 or 1/6

• final dilution 20/100 or 1/5

If 1 mL sample is put into tube #1 that has 49 mL of diluent, what is the tube dilution?

• 1/49

• 1/50

If 2 mL sample is removed from a tube with a dilution of 1/50 and placed into a tube #2 with 48 ML diluent, what is the tube dilution and final dilution for tube #2?

• tube dilution 2/48 or 1/24

• tube dilution 2/50 or 1/25

• final dilution 1/50

• final dilution 1/1250

If the concentration of the stock solution of 50 ng/ml, what is the concentration of a tube with final dilution of 1/1250?

• 0.2 ng/mL

• 50 ng/mL

• 0.04 ng/mL

• 2 ng/mL