Restriction Enzymes

Restriction Enzymes . . . 

• cut DNA at precise locations

• help bacteria to pick up DNA

• move DNA through a gel

• copy DNA

Restriction enzymes need the right temperature and buffer in order to work because . . . 

• they recreate the conditions from the bacteria at which the enzyme works 

• they cut the DNA

• they allow the DNA to move through the gel 

• they aren't needed - it's just a waste of time

Some restriction enzymes produce "sticky ends". Sticky ends are . . . 

• single-stranded DNA "overhangs" that can bind to complementary DNA

• smooth cuts that are sticky due to the "goo" left behind by the enzyme

• The two wires (black and red) on a gel box

• The surface of the LB agar plates so that bacteria can grow

What is the role of a restriction enzyme in making a DNA Fingerprint?

• to identify the number of nucleotides in every single DNA fragment in order to determine where to cut.

• to cut DNA in half

• to locate specific DNA sequences and cut them in order to form different sized fragments. This allows the DNA to be loaded into the chambers found on an agarose gel.

In order to insert a human gene into plasmid, both must 

• code for the same gene product.

• be cut by the same restriction enzyme.

• originate from the same type of cell.

• have identical sequences.

The DNA base sequences recognized by Endonuclease are called

• Restriction Site

• Recognition Site

• Fragmentation Site

• cutting Site

Who discovered Restriction Enzymes?

• Watson and Crick

• Arber ,Natha, Linn

• Jacob and Monod

• Beadle and Tatum

Lane 3

• EcoRI

• BamHI

• HaeIII

HaeIII cuts,fragments

• 2,2

• 1,2

• 1,1

• 1,3

Which was the first Restriction Enzyme discovered ?

• a).HindII

• b). EcoRI

• c).BamHI

• d).HindIII