Bacterial Transformation

What is genetic transformation?

• A prokaryotic cell turning into a eukaryotic cell

• Foreign DNA being inserted into a plasmid

• A host organism taking in foreign DNA

• A gene being mutated

Which is not a characteristic of prokaryotic cells?

• contains a nucleus

• contains plasmid DNA

• single-celled

• enclosed by a cell wall

How might a plasmid contribute to a selective advantage for a bacterial cell?

• Make the cell competent.

• Could provide a selective advantage for that cell.

• Make the cell wall stronger.

• Does not contribute

Tags

NGSS.HS-LS4-2

NGSS.HS-LS4-4

Cells are made competent by;

• The incubation of the bacteria at 95C

• A process that uses calcium chloride.

• A process that uses GFP

• A process that uses polymerases

LB containing ampicillin (2nd plate) is inoculated with the E. coli bacteria that contains ampicillin resistant gene. The bacteria will;

• Grow on the agar plate

• Will not grow on the agar plate.

• will only grow after the plate has been incubated at 45C

• will only grow after the plate has been placed in a warm water bath for 2 minutes.

Observe the results above. What gene is present in the engineered plasmid?

• kanamycin resistant gene

• ampicillin resistant gene

• tetracycline resistant gene

• No plasmid is necessary to grow on plate 3.

single celled organism that lacks a nucleus

• bacteria

• cell wall

• flagellum

• endospore

When fragments of DNA are connected from different sources, this creates:

• A plasmid

• A clone

• Recombinant DNA

• Fragmented DNA

What is the primary purpose of bacterial transformation in genetic engineering?

• To increase bacterial growth rate

• To introduce foreign DNA into a bacterial cell

• To remove plasmids from bacteria

• To create antibiotic resistance

Which of the following is a common method used to make bacterial cells competent for transformation?

• Heat shock treatment

• Ultraviolet radiation

• Electroporation

• Both A and C