PCR Quiz

You decide to use PCR to determine if you have Cystic Fibrosis, a genetic disorder. You then decide to also check if you are at risk for developing Alzheimers, another genetic disorder.
What PCR "ingredient" would be different in these two tests?

• Template DNA

• Nucleotides

• Taq DNA polymerase

• Primers

How many copies of DNA are made after 3 cycle of PCR?

• 2

• 4

• 8

• 16

In PCR, what is used to denature DNA

• helicase

• heat

• DNA polymerase

• DNA

At what temperature does the Extension step of PCR occur?

• 95°C

• 50-65°C

• 72°C

• Any temperature

At what temperature does the Denature step of PCR occur?

• 94-98° C

• 50-65° C

• 72° C

• Any temperature

What is the denaturation step?

• mutating RNA polymerase

• Cooling DNA to allow primers to form

• separating DNA helix in a cold environment

• separating DNA helix with using heat

Discuss two important applications of PCR in the field of science.

• Making proteins and RNA

• DNA cloning and sequencing, forensic science for DNA profiling

• Developing Enzymatic Reactions and Decomposition Reactions

• Organizing DNA by size and charge

Tags

NGSS.HS-LS1-1

Explain the use of PCR in forensic science and its significance.

• PCR is used to amplify small amounts of DNA for analysis in forensic science.

• PCR is used to create new DNA samples for forensic analysis.

• PCR is used to identify the age of the DNA sample in forensic science.

• PCR is used to extract DNA from the crime scene in forensic science.

Reverse transcription PCR uses

• mRNA as a template to form

• RNA as a template to form a DNA

• DNA as a template to form ssDNA

• all of these

What happens during the anneal step of PCR?

• Primers are created.

• The primers attach to the target DNA region.

• Primers copy the new DNA strand.

• Primers sequence DNA