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PCR Quiz

Authored by Lisa Thompson

Science

12th Grade

NGSS covered

PCR Quiz
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15 questions

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1.

MULTIPLE CHOICE QUESTION

1 min • 1 pt

You decide to use PCR to determine if you have Cystic Fibrosis, a genetic disorder. You then decide to also check if you are at risk for developing Alzheimers, another genetic disorder.
What PCR "ingredient" would be different in these two tests?

Template DNA

Nucleotides

Taq DNA polymerase

Primers

2.

MULTIPLE CHOICE QUESTION

1 min • 1 pt

How many copies of DNA are made after 3 cycle of PCR?

2

4

8

16

3.

MULTIPLE CHOICE QUESTION

1 min • 1 pt

In PCR, what is used to denature DNA

helicase

heat

DNA polymerase

DNA

4.

MULTIPLE CHOICE QUESTION

1 min • 1 pt

At what temperature does the Extension step of PCR occur?

95°C

50-65°C

72°C

Any temperature

5.

MULTIPLE CHOICE QUESTION

1 min • 1 pt

At what temperature does the Denature step of PCR occur?

94-98° C

50-65° C

72° C

Any temperature

6.

MULTIPLE CHOICE QUESTION

1 min • 1 pt

What is the denaturation step?

mutating RNA polymerase

Cooling DNA to allow primers to form

separating DNA helix in a cold environment

separating DNA helix with using heat

7.

MULTIPLE CHOICE QUESTION

1 min • 1 pt

Discuss two important applications of PCR in the field of science.

Making proteins and RNA

DNA cloning and sequencing, forensic science for DNA profiling

Developing Enzymatic Reactions and Decomposition Reactions

Organizing DNA by size and charge

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NGSS.HS-LS1-1

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