Isolation

Isolation of protoplasts mechanically from higher plants was pioneered by

• Klercher

• Tkebe et al

• Cocking

• Both A & B

The following are how an equipment is to be isolated EXCEPT _______________.

• Close main line valves to the equipment

• Block the pipe to the equipment

• Fill up the pipe with process liquid to the equipment.

• Drain the pipes to and from the equipment.

Who is responsible for executing/installing approved isolations before intrusive work?

• Contractors

• Area Authority (AA)

• Isolating Authority

• Isolating Operator (ISO)

Who is responsible for maintaining the Energy Isolation and Extended Period Isolation Registers?

• Contractors

• Area Authority (AA)

• Isolating Authority

• PTW Coordinator

What is the role of the Isolating Authority (ISA) in the Isolation Requirements section?

• Completing the Certificate Application

• Preparing the isolation requirements for process/instrumentation and mechanical isolations

• Reviewing the certificate for completeness

Who approves the isolation certificate and may require independent verification of isolation points?

• Certificate Applicant

• Area Authority (AA)

• Isolating Operator (ISO)

What does 'Zero Energy Demonstration' refer to in the certificate?

• Demonstrating equipment functionality

• Confirming that energy sources are isolated

• Showing energy consumption statistics

Tags

NGSS.HS-PS3-1

Isolation of DNA from cells is a crucial step in genetic engineering. Which of the following is NOT a common method for DNA isolation?

• Phenol-chloroform extraction

• Ethanol precipitation

• Centrifugation

• Electrophoresis

In microbiology, isolation of pure cultures is essential. Which technique is commonly used for isolating pure bacterial cultures?

• Streak plate method

• Gram staining

• PCR amplification

• Southern blotting

Which of the following is a key step in the isolation of mitochondria from eukaryotic cells?

• Homogenization

• Electrophoresis

• Spectrophotometry

• Chromatography