Agarose Gel Electrophoresis

What is the primary purpose of agarose gel electrophoresis?

• a) To amplify DNA

• b) To separate nucleic acids based on size

• c) To sequence DNA

• d) To denature proteins

Which of the following factors affects DNA migration in an agarose gel?

• a) DNA concentration

• b) Voltage applied

• c) Agarose concentration

• d) All of the answers

What is the function of ethidium bromide (EtBr) or SYBR Green in agarose gel electrophoresis?

• a) To cut DNA into fragments

• b) To stain DNA and make it visible under UV light

• c) To increase the speed of migration

• d) To denature DNA

Which buffer is commonly used in agarose gel electrophoresis?

• a) PBS

• b) Tris-Acetate-EDTA (TAE)

• c) SDS-PAGE

• d) Tris-Glycine

What charge does DNA have, and in which direction does it migrate during electrophoresis?

• a) Negative, migrates toward the anode (+)

• b) Positive, migrates toward the cathode (-)

• c) Neutral, remains in place

• d) Positive, migrates toward the anode (+)