Quiz 01

01.  Which of the following reagents is used to lyse cells during plasmid extraction?

• RNase

• SDS

• Ethanol

• TE buffer

2. 02. What is the main source of DNA in blood?

• Red blood cells

• Platelets

• Plasma

• White blood cells

3. 03. Which of the following is NOT an essential component of a standard PCR reaction?

• Primers

• DNA ligase

• MgCl₂

• dNTPs

4. 04. At what temperature does the annealing step usually occur?

• 95°C

• 72°C

• 50–65°C

• 37°C

5. 05. Iodine vapor is considered a non-permanent locating agent because

• It oxidizes compounds completely

• Spots fade over time

• It reacts only with acids

• It binds irreversibly to silica

A DNA sample with an A260/A280 ratio below 1.6 likely has ________ contamination

Name two next generation sequencing methods?

The following image shows the results of a Maxam-Gilbert sequencing experiment, visualized by autoradiography (X-ray film). Given the four lanes represent the four different chemical reactions (A+G, G, C, C+T), and assuming the DNA was labeled at the 5' end, what is the sequence of the DNA fragment? 

If you have RNA to run PCR, what you have to do first?

Name two gases use as mobile phase in Gas chromatography.