Glutamate Dehydrogenase Catabolism of Glutamate

The video tutorial explains the mechanism of glutamate dehydrogenase, focusing on oxidative deamination. It describes how glutamate is converted into ammonia and alpha-ketoglutarate, with ammonia entering the urea cycle and alpha-ketoglutarate entering the TCA cycle. The enzyme can use either NAD or NADP, and the process involves Schiff base formation and hydrolysis. The tutorial highlights the enzyme's mitochondrial location and its role in glutamate catabolism.

What is one of the main products of the oxidative deamination of glutamate?

• Urea

• Ammonia

• Glucose

• Lactate

Which molecule is involved in the initial oxidation step of glutamate dehydrogenase?

• Coenzyme A

• NAD or NADP

• FAD

• ATP

What is formed during the initial step of the mechanism involving glutamate dehydrogenase?

• A Schiff base

• A disulfide bridge

• A peptide bond

• A glycosidic bond

What is unique about the enzyme glutamate dehydrogenase?

• It only reacts with NAD+.

• It can react with both NADP+ and NAD+.

• It is only found in the cytoplasm.

• It does not require any cofactors.

Where is glutamate dehydrogenase highly expressed?

• In the cytoplasm

• In the endoplasmic reticulum

• In the nucleus

• In the mitochondrial matrix

What happens to the Schiff base during the final steps of the mechanism?

• It is methylated.

• It forms a peptide bond.

• It undergoes hydrolysis.

• It is phosphorylated.

What cycle does alpha-ketoglutarate enter after being produced?

• TCA cycle

• Glycolysis

• Pentose phosphate pathway

• Urea cycle