Aconitase 1

This video explores the organic mechanism of the enzyme aconitase in the TCA cycle. It details the mechanistic steps, including proton transfer and elimination reactions, and highlights the stereochemical aspects of the reaction, such as anti periplanar orientation and anti addition. The video concludes with the formation of isocitrate, emphasizing its unique stereochemistry.

What is another name for the enzyme aconitase?

• Aconitate hydratase

• Citrate synthase

• Isocitrate dehydrogenase

• Fumarase

What is the first step in the mechanism of aconitase?

• Oxidation of a substrate

• Hydration of a double bond

• Proton transfer

• Formation of an alkene

What type of elimination process is involved in the aconitase mechanism?

• E1

• SN2

• SN1

• E2

What is the configuration of the double bond in cis-aconitate?

• E

• Z

• R

• S

What happens to histidine in the final steps of the aconitase mechanism?

• It is reduced

• It is deprotonated

• It is oxidized

• It is re-protonated

What type of addition is observed in the formation of isocitrate?

• Radical addition

• Anti addition

• Nucleophilic addition

• Syn addition

Which molecule is specifically formed as a result of the aconitase reaction?

• Citrate

• Malate

• Isocitrate

• Fumarate