Glycolysis | Regulation of PFK-1/Glycolysis Via PFK-2 Activity

The video explains the regulation of glycolysis and gluconeogenesis through the enzyme phosphofructokinase-1 (PFK-1). It highlights the role of fructose 2,6-bisphosphate as an allosteric activator of PFK-1, influenced by the hormones insulin and glucagon. Insulin promotes glycolysis by activating PFK-2, which increases fructose 2,6-bisphosphate levels, while glucagon favors gluconeogenesis by activating fructose bisphosphatase 2, reducing fructose 2,6-bisphosphate levels. The balance between these hormones determines the metabolic pathway preference, ensuring proper blood glucose regulation.

What is the significance of the phosphorylation state of the bifunctional enzyme?

How does the concentration of fructose 2,6-bisphosphate influence the metabolic pathway in a cell?

Discuss the interplay between glycolysis and gluconeogenesis in response to hormonal signals.

What are the consequences of elevated blood glucose levels on insulin secretion and glycolysis?