Corrin/B12 Biochemistry: Cobalamin Adenosyltransferase

The video tutorial explores the transformations of vitamin B12, focusing on its role in human metabolism. It highlights two main enzymes, methionine synthase and methylmalonyl COA mutase, that utilize B12. The tutorial delves into the function of cobalamin adenosyl transferase, explaining how it converts B12 into a form used by methylmalonyl COA mutase. The video also discusses B12's unique properties as a super nucleophile, the significance of its ligands, and the electron transfer mechanism involved in its metabolic processes.

What are the two main enzymes in humans that react with vitamin B12?

Explain the role of adenosine triphosphate (ATP) in the reaction involving methylmalonyl COA mutase.

Describe the significance of the cobalt in the plus one oxidation state in relation to its nucleophilicity.

Discuss the importance of the Corin ring in the structure of vitamin B12.

What is the difference between the alpha ligand and beta ligand in the context of vitamin B12?

How does the deoxy adenosyl group become the beta ligand in the B12 molecule?

What is the role of the leaving group in the electron transfer from cobalt to carbon?

Explain the process of how triphosphate is cleaved during the reaction involving vitamin B12.

What happens to the cobalt ion during the electron transfer process in the enzymatic reaction?

What is the final product formed from the reaction involving cobalamin adenosyl transferase?