Understanding Cloning in a Scientific Context

What is the first step in cloning a gene scientifically?

• Isolating the DNA from the gene of interest

• Transforming the gene into a plasmid

• Adding antibiotics to the gene

• Isolating the messenger RNA from the gene of interest

What enzyme is used to convert RNA into complementary DNA?

• RNA polymerase

• Ligase

• Reverse transcriptase

• DNA polymerase

What is the purpose of transforming complementary DNA into a plasmid?

• To destroy the DNA

• To make the DNA resistant to viruses

• To amplify the DNA by inserting it into a plasmid

• To convert the DNA back into RNA

Why are antibiotic-resistant genes included in the plasmid?

• To make the plasmid visible under a microscope

• To make the bacteria grow faster

• To ensure only bacteria with the plasmid survive

• To prevent the plasmid from being destroyed

What happens to the bacteria after they are successfully transfected with the plasmid?

• They stop replicating

• They produce large amounts of the gene of interest

• They become resistant to all antibiotics

• They die off quickly