Hybridoma Technology Quiz

What is a key difference between monoclonal and polyclonal antibodies?

• Polyclonal antibodies are identical.

• Polyclonal antibodies are derived from a single B-cell.

• Monoclonal antibodies bind to multiple epitopes.

• Monoclonal antibodies originate from a single B-cell.

Why are monoclonal antibodies often used in cancer treatment?

• They are produced by multiple B-cells.

• They can bind to multiple cancer cells.

• They are less expensive to produce.

• They are identical and target a specific epitope.

What is the purpose of injecting an antigen into a mouse in hybridoma technology?

• To make the mouse immune to all diseases.

• To provoke an immune response and produce antibodies.

• To directly produce monoclonal antibodies.

• To create myeloma cells.

What is the role of myeloma cells in hybridoma technology?

• They are immortal cells that help in cell fusion.

• They are used to evaluate antibody specificity.

• They directly produce antibodies.

• They are used to produce antigens.

How is the specificity of monoclonal antibodies evaluated?

• By using a selective medium.

• Through ELISA or Western blot techniques.

• By fusing them with myeloma cells.

• By injecting them into mice.