Wayground logo

Free Printable Worksheets

Font size

S
M
L
XL
Worksheets

Elisa/Immune system lab

Total questions: 58

Worksheet time: 38mins

Name
Class
Date
1.
What does the S in ELISA stand for?
a)
substrate
b)
stationary
c)
sorbent
d)
starch
2.
What are practical uses for the ELISA test?
a)
pathogens
b)
antigens
c)
AIDS
d)
All of the above
3.
What is a primary antibody?
a)
First step in ELISA that binds to the antigen
b)
The first antibody
c)
The most important antibody
d)
a chicken antibody
4.
What type of antibody test can you purchase at your local pharmacy?
a)
Diabetes
b)
Pregnancy
c)
blood pressure
d)
temperature
5.
Give an example of a disease that attacks the immune system.
a)
Lupus
b)
Pneumonia
c)
MRSA
d)
Diabetes
6.
Why are immunosuppressant drugs necessary when someone has an organ transplant?
a)
To help like a vitamin
b)
To prevent the body from treating the organ like an invader
c)
To help increase the blood vessels to the transplanted organ
d)
To create homeostasis
7.
Why are enzymes used in this assay?
a)
They link to the proteins used
b)
The catalyze reactions
c)
They provide a detection method to see if the antibodies have attached. 
d)
They are used to detect DNA
8.
Why did you need to wash the wells after every step?
a)
To remove unbound proteins so there will be no false positive results
b)
To clean out the wells to see the color change
c)
Because it is fun
d)
To target bacteria that may stick to the plastic
9.
Why did you assay your samples in triplicate?
a)
To act as a control of the assay
b)
Reproducibility of results
c)
To test for pipetting errors
d)
All of the above
10.
Antibodies not only protect us from infections, they can also be used in laboratories to determine whether or not a patient has or has had a disease.
a)
TRUE
b)
FALSE
11.
When a positive + result occurs ELISA, then one could conclude that...
a)
a + result always means illness
b)
the antibody is present
c)
it is a false + and no illness
d)
an enzyme is present 
12.
What is a primary antibody?
a)
the second antibody used to detect foreign particle
b)
particle produced by the antigen
c)
the first antibody used to detect foreign particle
d)
second cell produced by the macrophage
13.
Why is a positive and negative control necessary in the setup of ELISA?
a)
positive controls are equal to negative controls 
b)
ELISA is subject to errors; if controls fail the results are untrustworthy
c)
ELISA is a well run test that typically does not need controls
d)
negative and positive controls are needed to exclude all results
14.
Why is it necessary to wash the samples repeatedly?
a)
it is important to keep the lab wells clean
b)
it is important to wash away all bound and unbound antibodies from teh wells
c)
it is important to wash away the unbound antibodies
d)
washing the wells is just solid lab practice
15.
What does the symbol μL stand for?
a)
nanoliters
b)
milliters
c)
microliters
d)
muliters
16.
From what cells do antibodies originate?
a)
b cells
b)
t cells 
c)
prison cells
d)
red blood cells
17.
Which of the following are commonly tested for using the ELISA method?
a)
HIV
b)
Pregnancy
c)
Lyme Disease
d)
All of these
18.
The basic 4 steps of the ELISA are:
bind sample to well
add primary Ab and wash
add secondary Ab/enzyme and wash
add substrate
a)
TRUE
b)
FALSE
19.

Why are enzymes used in this immunoassay?

a)

for color change

b)

to protect the antigen

c)

to denature protein

d)

to speed the reaction

20.
What would be the tube dilution?
a)
1/10
b)
2/10
c)
1/100
d)
1/1000
21.
What would be the final dilution?
a)
1/10
b)
1/100
c)
1/1000
d)
1/10000
22.
What is another name for antibodies?
a)
helper t cells
b)
hemoglobin
c)
immunoglynocists
d)
immunoglobulins
23.
During an immune response, which of the following is the first cell responder to attack a pathogen's antigen?
a)
B cell
b)
T cell
c)
macrophage
d)
leukocyte
24.

During the virtual lab (and eventually our ELISA lab), you will prepare a set of dilutions...why?

a)

serial dilutions help determine the level of antibodies present in a sample

b)

serial dilutions identify the pathogen

c)

serial dilutions help determine the level of macrophages present

d)

serial dilutions identify the killer T cells present

25.

The first layer on the ELISA plate was the

a)

antigen

b)

antibody

c)

virus

d)

substrate

26.

The A in ELISA stands for

a)

allele

b)

antigen

c)

assay

d)

antibody

27.

What we attempting to detect in this ELISA?

a)

SARS

b)

HIV

c)

HPV

d)

MRSA

28.

Which one of the following is NOT one of the nonspecific body defenses?

a)

mucous membranes

b)

antibody production

c)

natural killer cells

d)

inflammatory response

29.

Long lived B cell clone members that do not become plasma cells but are capable of an immune response against the same antigen at a later time are called:

a)

killer cells

b)

natural killers

c)

helper cells

d)

memory cells

30.

What is the term for a protein that can put holes in bacteria and cause them to lyse?

a)

Phagocyte

b)

natural killer cell

c)

complement

d)

interferon

31.
What structures are foreign substances that invade the body?
a)
neutrophils
b)
lymphocytes
c)
phagocytes
d)
antigens
32.
Cells that ingest pathogens are
a)
plasma cells
b)
phagocytic cells
c)
helper T cells
d)
antibodies
33.
What do scientists call bacteria and viruses that can make people sick?  
a)
diseases
b)
vaccines
c)
antibodies
d)
pathogens
34.
Y shaped proteins that may mark pathogens for destruction are
a)
antigens
b)
T cells
c)
antibodies
d)
neutrophils
35.
What do B cells produce?
a)
antigens
b)
macrophages
c)
T cells
d)
antibodies
36.
Pathogens are recognized by their...
a)
antigens
b)
antibodies
c)
killer t-cells
d)
B-cells
37.

A secondary immune response will be faster when...

a)

The individual has encountered the pathogen already

b)

The individual's macrophages are on high alert

c)

The individual has encountered the pathogen and has memory B cells circulating

d)

Antibodies are produced by T cells

38.

What is the function of B cell

a)

Proliferate plasma cell and memory B cell

b)

Release antibodies

c)

Secrete interleukin-2

d)

Produce interleukin-1

39.

T lymphocyte is used in which immune response

a)

Humoral Immune Response

b)

Cell Mediated Immune Response

c)

Antibody-Mediated Immune Response

40.

How do T cells differ from B cells?

a)

T cells fight pathogens by killing pathogens directly, while B cells fight pathogens by making antibodies.

b)

T cells only make memory cells, and B cells only make antigens

c)

T cells fight pathogens by making antibodies, while B cells fight pathogens by killing pathogens directly.

d)

T cells can only fight off pathogens the body has encountered once, and B cells can fight off any pathogen the body comes across.

41.
In antibody mediated immunity which statement is NOT true?
a)
B lymphocytes are sensitized by an antigen
b)
Memory cells are produced
c)
Plasma cells are produced
d)
B lympocytes respond to infected cells
42.
What enhances the ability of antibodies and phagocytic cells to clear microbes and damaged cells from an organism, promote inflammation and attacks the pathogen's plasma membrane?
a)
Interferon
b)
Integration
c)
Enhancement System
d)
Complement System
43.

Which of the following is a pathogen?

a)

Bacteria only

b)

Viruses, bacteria, fungi, and Protista

c)

Viruses and bacteria

44.

______________________ attack and kill infected cells

a)

Helper T Cells

b)

B Cells

c)

Cytotoxic T Cells

45.

Acquired immunodeficiency syndrome

a)

Koch's Postulates

b)

Immunity

c)

Vaccine

d)

Autoimmune disease

e)

AIDS

46.

___: immune response: uses B cells

a)

Humoral

b)

Inflammation

c)

Histamine

d)

Tonsils

47.
What type of cell does HIV destroy?
a)
b-helper cell
b)
WBC
c)
T-helper cells
d)
RBc
48.
To transmit HIV the virus must enter what part of the body?
a)
stomach
b)
bloodstream
c)
mouth
d)
lymph nodes
49.
The HIV virus attacks and weakens this system
a)
immune system
b)
digestive system
c)
circulatory system
d)
skeletal system
50.
How does HIV interact with T-cells?
a)
It bypasses the T cells and begins reproducing in the bloodstream
b)
It directly attacks the T cells
c)
It begins producing T cells of its own
d)
It disguises itself as a T cell 
51.

A positive ELISA test always indicates that an individual is sick.

a)

True

b)

False

52.

An HIV test looks for these "markers" that signal the presence of HIV

a)

Microbes

b)

Pathogens

c)

Antibodies

d)

Red Blood Cells

53.

Antibodies are defined as

a)

Special molecules produced by the bodies lymph nodes

b)

Special glucose molecules produced by the bodies immune system

c)

Special proteins produced by the bodies immune system

d)

None of the above

54.

The Elisa Test is typically the first test completed in order to diagnose HIV

a)

True

b)

False

55.
HIV stands for
a)
Human Inverted Virus
b)
Human Internal Virus
c)
Human Immunodeficiency Virus
d)
Human Integrated Virus
56.
AIDS stands for:
a)
Acquired inverted deficiency syndrome
b)
Acquired immuno deficiency syndrome
c)
Accidental internal deficiency syndrome
d)
Adapted indicator dehydrated syndrome
57.
HIV is the virus that causes AIDS
a)
TRUE
b)
FALSE
58.
When CD4 cells (immune system cells) drop below a certain number, what happens?
a)
The person now has HIV
b)
HIV has now become AIDS
c)
The person is cured

Similar Resources on Wayground

INTERAKSI ANTAR KOMPONEN EKOSISTEM

60 questions

INTERAKSI ANTAR KOMPONEN EKOSISTEM

quiz

Worksheet

•

12th Grade - University

Ch. 2 Chem Review for BIO

60 questions

Ch. 2 Chem Review for BIO

quiz

Worksheet

•

12th Grade - University

Biol110 Exam 1

54 questions

Biol110 Exam 1

quiz

Worksheet

•

University

BioD

62 questions

BioD

quiz

Worksheet

•

12th Grade - University

Final Exam Review Ch 10, 12-15

55 questions

Final Exam Review Ch 10, 12-15

quiz

Worksheet

•

University

Mills: Reproductive System

55 questions

Mills: Reproductive System

quiz

Worksheet

•

9th Grade - University

Respiratory System

63 questions

Respiratory System

quiz

Worksheet

•

11th Grade - University

Unit 11

58 questions

Unit 11

quiz

Worksheet

•

KG - University