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Sterilization and disinfection (section 2,3)

Total questions: 40

Worksheet time: 7mins

Name
Class
Date
1.

The cleaning process that is required for all instruments that penetrate the skin or that come in contact with normally sterile areas of the tissue and internal organs is

a)

sterilization

b)

sanitization

c)

ultrasonic cleaning

d)

disinfection

2.

Which of the following is a limitation of the autoclave?

a)

It lacks the ability to kill endospores

b)

It lacks the ability to inactivate viruses

c)

It cannot be used with glassware

d)

It will destroy some heat labile materials

e)

It takes too long to sterilize

3.

All of the following disinfectant contains heavy metal EXCEPT:

a)

chlorine

b)

silver nitrate

c)

Merthiolate

d)

Mercurochrome

e)

copper sulphate

4.

Identify the term that is used to ensure surgical instruments are free from micro-organisms:

a)

Debrided

b)

Cleaned

c)

Disinfected

d)

Sterilization

e)

Sanitization

5.

Each of the following statements concerning the killing of bacteria is correct EXCEPT:

a)

An autoclave uses steam under pressure to reach the killing temperature of 121°C

b)

A 70% solution of ethanol kills more effectively than absolute (100%) ethanol.

c)

The pasteurization of milk kills pathogens but allows many organisms and spores to survive.

d)

Iodine kill bacteria by by point mutation (thymine dimer formation) so need exposure for long time

6.

Which of the following is used to disinfect surfaces and soak rubber equipment?

a)

iodine

b)

alcohols

c)

dyes

d)

bleach (sodium hypochlorite)

7.

Which of the following is commonly used as chemical disinfectant in the laboratory setting to provide a measure of protection against transmission of the human immunodeficiency virus (HIV)?

a)

iodine

b)

bleach

c)

germicidal soap

d)

hot air

8.

Which of the following is most effective for sterilizing plastic Petri plates?

a)

Autoclave

b)

Glutaraldehyde

c)

Chlorine

d)

gamma rays

e)

Ultraviolet radiation

9.

Which of the following methods is useful for sterilization of antisera?

a)

Autoclaving

b)

Hot air oven

c)

Tyndallisation

d)

Filtration

10.

All of the following are sporicidal except

a)

alcohol

b)

autoclave

c)

chlorine

11.

Any process that destroys the non-spore-forming contaminants on inanimate objects is:

a)

Disinfection

b)

antisepsis

c)

degermation

d)

sterilization

12.

Any process that destroys the non-spore-forming contaminants on animate objects is:

a)

Disinfection

b)

antisepsis

c)

degermation

d)

sterilization

13.

Which of the following is most resistant to antiseptics/sterilization:

a)

prion

b)

bacterial spores

c)

cyst

d)

fungus

14.

Which of the following is least resistant to antiseptics/sterilization:

a)

prion

b)

bacterial spores

c)

enveloped viruses

d)

naked viruses

15.

Which of the following is used as antiseptic but its effectiveness is limited by the presence of blood products, mucus, or soap (organic matter)?

a)

iodine

b)

formalin 10%

c)

phenol compounds

d)

formaldehyde gas

16.

Which concept is correct about the effectiveness of a disinfectant?

a)

using soap in the sanitization process is desirable

b)

evaporation can alter the chemical makeup of the solution

c)

items should be placed in the disinfectant bath wet

d)

the disinfectant solution is more potent after several uses

17.

Which is a form of cold sterilization

a)

gamma rays

b)

UV rays

c)

infrared rays

d)

flaming

e)

steam sterilization

18.

Identify the term that can describe a disinfectant that can inhibit the growth of fungi:

a)

fungicidal

b)

bactericidal

c)

virucidal

d)

fungistatic

19.

The most suitable way of sterilization of oils and fats is:

a)

Tyndallisation

b)

Pasteurization

c)

Hot air oven

d)

Autoclaving

20.

Which of the following items could be sterilized by dry heat sterilization?

a)

glass pipette

b)

Intravenous (IV) solution

c)

rubber gloves

d)

Plastic IV bags

e)

plastic petri dishes

21.

If a 1:600 dilution of a test compound kills a standard population of Staphylococcus aureus in 10 minutes but not 5 minutes while a 1:60 dilution of phenol kills the population in the same time, what is the phenol coefficient of the test compound?

a)

1

b)

5

c)

100

d)

50

e)

10

22.

Which of the following was the first widely used antiseptic and disinfectant and used in standardization of new disinfectants?

a)

phenol

b)

iodine

c)

crystal violet

d)

alcohol

23.

Tyndallization is a type of

a)

Intermittent sterilization

b)

Pasteurization

c)

Dry heat

d)

Boiling

24.

Which of the following can be disinfected and reused without sterilization?

a)

surgical instruments

b)

endoscope

c)

nail clippers

d)

syringes and needles

25.

The efficiency of moist-heat sterilization is tested with

a)

Clostridium botulinum

b)

Spores of aerobic bacteria

c)

Spores of Bacillus stearothermophilus

d)

spores of Bacillus anthracis

26.

Sharp instruments should not be sterilized by:

a)

Autoclave

b)

Hot Air Oven

c)

Boiling

d)

all of the above

27.

Complete killing or removal of all types of micro-organisms, i.e. bacteria, viruses, fungi and mycobacteria is called;

a)

sterilization

b)

decontamination

c)

disinfection

d)

antisepsis

28.

Browne’s tube is used as indicator for efficacy of:

a)

Heat sterilization

b)

Chemical sterilization

c)

Filtration

d)

Ultraviolet rays

29.

Which of the following methods of infection control requires the use of an autoclave?

a)

sterilization

b)

sanitization

c)

disinfection

d)

irradiation

30.

Which of the following method is best to sterilize heat labile solutions?

a)

Hot Air Oven

b)

Autoclave

c)

Membrane filtration

d)

tyndallization

31.

Which of the following substances can sterilize?

a)

alcohol

b)

dyes

c)

0.3%formalin

d)

0.5%phenol

e)

none of the above

32.

The time at a specific temperature needed to kill a population of cells is

a)

contact time

b)

decimal reduction point

c)

time temperature combination (f value)

d)

thermal death point

33.

vertical......>100c

a)
b)
c)
d)
34.

dry.........heat

a)
b)
c)
d)
35.

moist.........heat

a)
b)
c)
d)
e)
36.

chemical........sterilization

a)
b)
c)
d)
37.

hotness till redness

a)
b)
c)
d)
38.

sonicator

a)
b)
c)
d)
e)
39.

HAO

a)
b)
c)
d)
40.

Seitz

a)
b)
c)
d)

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