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Isolation techniques, Macrodilution and Colony count

Total questions: 32

Worksheet time: 16mins

Name
Class
Date
1.

True or False


Isolation technique is process whereby microorganism are separated from each other and cultivated in the medium.


Once pure culture are made, they will subject to colony count to estimate Colony Factor Unit (CFU)/mL and Bacterial count/mL.

a)

1st statement is true, 2nd statement is false

b)

1st statement is false, 2nd statement is true

c)

Both statement is true

d)

Both statement is false

2.

It is a type of technique that uses cotton swab or inoculating loop and primary quadrant is diluted into secondary, tertiary so the individual colonies may be formed?

a)

Pour plate

b)

Hot plate

c)

Spread plate

d)

Streak plate

3.

The sample is pipetted onto the surface of agar plate, after incubation the typical microorganism is aerobic.

a)

Streak plate

b)

Pour plate

c)

Spread plate

d)

Hot plate

4.

It can be used to determine the number of microbes/ml in a specimen, it often used to assay bacterial contamination of foodstuff. Obligate aerobes may grow poorly.

a)

Pour plate

b)

Hot plate

c)

Spread plate

d)

Streak plate

5.

All strokes of the loop or needle are done in a single forward direction.

a)

A.K.A Christmas tree

b)

Multiple Interrupted Streak

c)

Lawn Streak Technique

d)

Streak pattern by Pelczar and Reid

6.

Total volume/ Volume of aliqout

a)

Total Volume

b)

Dilution

c)

Dilution factor

d)

Bacteria/mL

7.

Volume solute/ Total Volume

a)

CFU/mL

b)

Dilution

c)

Dilution factor

d)

Bacteria/mL

8.

Colony count multiply by reciprocal of dilution

a)

CFU/mL

b)

Dilution

c)

Total Volume

d)

Bacteria/mL

9.

The number of colonies counted multiply by conversion factor of inoculating loop

a)

CFU/mL

b)

Dilution

c)

Total Volume

d)

Bacteria/mL

10.

Example of Dilution Factor

a)

10

b)

10:1

c)

1/10mL

d)

1mL

11.

Interpret the result:

35,657 CFU/ml

a)

Clinical Bacteriuria

b)

Suggestive

c)

Contamination

12.

Interpret the result:

7,000 CFU/ml

a)

Contamination

b)

Significant

c)

Suggestive

13.

Interpret the result:

235,000 CFU/ml

a)

Contamination

b)

Significant

c)

Suggestive

14.

T or F


In the pour-plate technique, after diluted several times where the last test tube is T10, the next step is to get another test tube to perform the last procedure.

a)

False

b)

True

15.

T or F


In the pour-plate technique, after diluted several times where the last test tube is T10, the next step is to get another test tube to perform the last procedure.

a)

True

b)

False

16.

The advantage of pour plates is that embedded colonies are too much smaller than those which happen to be on the surface.


One disadvantage is that it doesn't require previously prepared plates.

a)

1st statement is true, 2nd statement is false

b)

1st statement is false, 2nd statement is true

c)

Both statement is true

d)

Both statement is false

17.

What is the best thing to do if the result of the Colony Forming Unit (CFU) is suggestive?

a)

Report the result

b)

Repeat using first morning urine

c)

Incubate for 37 degree C for 24 hour

d)

get another sample in the broth and repeat the procedure twice

18.

Measure of rate of killing of bacteria by an antimicrobial agent?

a)

Time-kill assay

b)

Synergy test

c)

Serum bactericidal test (SBT)

d)

Minimum bactericidal concentrattion (MBC) test

e)

Antimicrobial concentration test (assay)

19.

Measures the susceptibility of a bacterial isolate to a combination of two or more antimicrobial agents?

a)

Time-kill assay

b)

Synergy test

c)

Serum bactericidal test (SBT)

d)

Minimum bactericidal concentrattion (MBC) test

e)

Antimicrobial concentration test (assay)

20.

Measure of highest dilution or titer of a patient's serum that is inhibitory to patient's own infecting bacterium and highest dilution or titer that is bactericidal?

a)

Time-kill assay

b)

Synergy test

c)

Serum bactericidal test (SBT)

d)

Minimum bactericidal concentration (MBC) test

e)

Antimicrobial concentration test (assay)

21.

Measure of lowest concentration of antimicrobial agent that kills a bacteria isolate?

a)

Time-kill assay

b)

Synergy test

c)

Serum bactericidal test (SBT)

d)

Minimum bactericidal concentrattion (MBC) test

e)

Antimicrobial concentration test (assay)

22.

Measure amount of antimicrobial agent in serum or body fluid?

a)

Time-kill assay

b)

Synergy test

c)

Serum bactericidal test (SBT)

d)

Minimum bactericidal concentrattion (MBC) test

e)

Antimicrobial concentration test (assay)

23.

It is a tube that is needed for MIC test, it is a test for bacterial suspension that has turbidity?

a)

0.5 McFarland Standard

b)

Sterile test tube

c)

Growth control tube

d)

Durham's tube

e)

MIC 0.8% tube testing

24.

True or False


MIC is a test for identify the amount of antimicrobial agent require to enhance the bacterial growth.


In clinical situations it needs to be cidal to affect a successful outcome for the patient.

a)

1st statement is true, 2nd statement is false

b)

1st statement is false, 2nd statement is true

c)

Both statement is true

d)

Both statement is false

25.

Is defined as the lowest concentration of antimicrobial agents that showed no growth or no turbidity?

a)

Antibiotic-antimicrobial concentration

b)

Low concentration technique

c)

MBC concentration

d)

End point tube

26.

VRE Stands for

a)

Vancomycin-Resilient Enterococci

b)

Vancomycin-Resistant Enterotoxins

c)

Vancomycin-Resilient Enterotoxins

d)

Vancomycin-Resistant Enterococci

27.

All of the following is true for MBC except:

a)

Reported in ug/mm

b)

no growth after subcultured on NA

c)

bactericidal

d)

Lowest concentration of antimicrobial agent

28.

It is impregnated with various concentration of antibiotics?

a)

Antibiotic drug concentration

b)

Broth control

c)

E-strips

d)

Multiple drug resistant

29.

Antimicrobial agents that are bactericidal for Escherichia coli?

a)

MRSA

b)

VRE

c)

ESBLs

d)

PRSP

30.

What tube is the dilution for MBC?

a)

T4

b)

T5

c)

T6

d)

T7

31.

What tube is the dilution for MIC?

a)

T4

b)

T5

c)

T6

d)

T7

32.

True or False


You can perform MBC test without MIC test.


In performing MBC test, it is performed only in broth.

a)

1st statement is true, 2nd statement is false

b)

1st statement is false, 2nd statement is true

c)

Both statement is false

d)

Both statement is true