WorksheetsIsolation techniques, Macrodilution and Colony count
Total questions: 32
Worksheet time: 16mins
True or False
Isolation technique is process whereby microorganism are separated from each other and cultivated in the medium.
Once pure culture are made, they will subject to colony count to estimate Colony Factor Unit (CFU)/mL and Bacterial count/mL.
1st statement is true, 2nd statement is false
1st statement is false, 2nd statement is true
Both statement is true
Both statement is false
It is a type of technique that uses cotton swab or inoculating loop and primary quadrant is diluted into secondary, tertiary so the individual colonies may be formed?
Pour plate
Hot plate
Spread plate
Streak plate
The sample is pipetted onto the surface of agar plate, after incubation the typical microorganism is aerobic.
Streak plate
Pour plate
Spread plate
Hot plate
It can be used to determine the number of microbes/ml in a specimen, it often used to assay bacterial contamination of foodstuff. Obligate aerobes may grow poorly.
Pour plate
Hot plate
Spread plate
Streak plate
All strokes of the loop or needle are done in a single forward direction.
A.K.A Christmas tree
Multiple Interrupted Streak
Lawn Streak Technique
Streak pattern by Pelczar and Reid
Total volume/ Volume of aliqout
Total Volume
Dilution
Dilution factor
Bacteria/mL
Volume solute/ Total Volume
CFU/mL
Dilution
Dilution factor
Bacteria/mL
Colony count multiply by reciprocal of dilution
CFU/mL
Dilution
Total Volume
Bacteria/mL
The number of colonies counted multiply by conversion factor of inoculating loop
CFU/mL
Dilution
Total Volume
Bacteria/mL
Example of Dilution Factor
10
10:1
1/10mL
1mL
Interpret the result:
35,657 CFU/ml
Clinical Bacteriuria
Suggestive
Contamination
Interpret the result:
7,000 CFU/ml
Contamination
Significant
Suggestive
Interpret the result:
235,000 CFU/ml
Contamination
Significant
Suggestive
T or F
In the pour-plate technique, after diluted several times where the last test tube is T10, the next step is to get another test tube to perform the last procedure.
False
True
T or F
In the pour-plate technique, after diluted several times where the last test tube is T10, the next step is to get another test tube to perform the last procedure.
True
False
The advantage of pour plates is that embedded colonies are too much smaller than those which happen to be on the surface.
One disadvantage is that it doesn't require previously prepared plates.
1st statement is true, 2nd statement is false
1st statement is false, 2nd statement is true
Both statement is true
Both statement is false
What is the best thing to do if the result of the Colony Forming Unit (CFU) is suggestive?
Report the result
Repeat using first morning urine
Incubate for 37 degree C for 24 hour
get another sample in the broth and repeat the procedure twice
Measure of rate of killing of bacteria by an antimicrobial agent?
Time-kill assay
Synergy test
Serum bactericidal test (SBT)
Minimum bactericidal concentrattion (MBC) test
Antimicrobial concentration test (assay)
Measures the susceptibility of a bacterial isolate to a combination of two or more antimicrobial agents?
Time-kill assay
Synergy test
Serum bactericidal test (SBT)
Minimum bactericidal concentrattion (MBC) test
Antimicrobial concentration test (assay)
Measure of highest dilution or titer of a patient's serum that is inhibitory to patient's own infecting bacterium and highest dilution or titer that is bactericidal?
Time-kill assay
Synergy test
Serum bactericidal test (SBT)
Minimum bactericidal concentration (MBC) test
Antimicrobial concentration test (assay)
Measure of lowest concentration of antimicrobial agent that kills a bacteria isolate?
Time-kill assay
Synergy test
Serum bactericidal test (SBT)
Minimum bactericidal concentrattion (MBC) test
Antimicrobial concentration test (assay)
Measure amount of antimicrobial agent in serum or body fluid?
Time-kill assay
Synergy test
Serum bactericidal test (SBT)
Minimum bactericidal concentrattion (MBC) test
Antimicrobial concentration test (assay)
It is a tube that is needed for MIC test, it is a test for bacterial suspension that has turbidity?
0.5 McFarland Standard
Sterile test tube
Growth control tube
Durham's tube
MIC 0.8% tube testing
True or False
MIC is a test for identify the amount of antimicrobial agent require to enhance the bacterial growth.
In clinical situations it needs to be cidal to affect a successful outcome for the patient.
1st statement is true, 2nd statement is false
1st statement is false, 2nd statement is true
Both statement is true
Both statement is false
Is defined as the lowest concentration of antimicrobial agents that showed no growth or no turbidity?
Antibiotic-antimicrobial concentration
Low concentration technique
MBC concentration
End point tube
VRE Stands for
Vancomycin-Resilient Enterococci
Vancomycin-Resistant Enterotoxins
Vancomycin-Resilient Enterotoxins
Vancomycin-Resistant Enterococci
All of the following is true for MBC except:
Reported in ug/mm
no growth after subcultured on NA
bactericidal
Lowest concentration of antimicrobial agent
It is impregnated with various concentration of antibiotics?
Antibiotic drug concentration
Broth control
E-strips
Multiple drug resistant
Antimicrobial agents that are bactericidal for Escherichia coli?
MRSA
VRE
ESBLs
PRSP
What tube is the dilution for MBC?
T4
T5
T6
T7
What tube is the dilution for MIC?
T4
T5
T6
T7
True or False
You can perform MBC test without MIC test.
In performing MBC test, it is performed only in broth.
1st statement is true, 2nd statement is false
1st statement is false, 2nd statement is true
Both statement is false
Both statement is true
