You have made a new culture of a Streptococcus species in a slanted test tube. After a few days, you notice less and less new growth. What is the most likely reason for the slow down of growth?

You are trying to culture Thiobacillus prosperus, a known acidophile. Your lab instructor tells you how to prepare the growth medium and how long to incubate the culture but you end up with half the growth of your classmates. What is the most likely explanation for what you did wrong?

After the at home hand washing experiment, you decide to see what happens to the bacterial culture over time. You place the culture in a sealed plastic bag and put it in a warm, dark place. After three months you attempt to make a new culture from a sample of the original culture and find that the new culture still grows. What is the likely stage that the original culture was in when you sampled it?

Nitrifying bacteria are an important part of quality garden soil. You wonder what stage of growth the bacteria in your garden are in. What statement is UNTRUE about the growth you would expect?

You are trying to isolate a common bacteria species that is found in natural aquatic environments. Although you are able to grow the bacteria in mixed culture, the bacteria is impossible to grow in pure culture. What best explains why this might be?

You have three half gallons of milk that are past their sell-by date and you want to test if the date really indicates if it is unsafe to drink. What is NOT a method you might use to determine if it is safe to drink?

Penicillin was derived originally from common moulds known as Penicillium moulds which produced antibiotic compounds. Do you think that bacteria also produce antibiotic compounds?

In what stage of biofilm formation do you expect quorum sensing that generates EPS to be the most active?

A bacterial species in a marsh environment is only found deep in biofilms. What theory best explains why this might be?

Where are biofilms generally not a concern in medical settings?

Psychrotrophs are generally disease causing.

In the lab, you make two cultures of the same bacterial species. One culture, you incubate in a specialized anaerobic jar, the other culture you incubate normally. Both cultures appear to have the same amount of growth. What is the classification of this bacterial species?

Pickling with vinegar has been used to preserve food for centuries. Why does this process prevent bacterial growth?

You know that you can preserve food with lots of salt. If you got a cut, would it be effective to sterilize it by literally putting salt in the wound?

All soil able to support plant life has bacteria in it. In dry places like deserts, what kind of bacteria would you be likely to find in the soil?

You make two cultures of Staphylococcus aureus. One culture you make will a general purpose medium, the other you make with a high saline content growth medium. Both cultures grow but the one with saline has less growth than the one without. What would best classify S. aureus?

Carbon accounts for 90% of dry cell weight

Where would cells generally not use nitrogen?

Superoxide dismutase works by changing a superoxide free radical to what less toxic compound?

Enzymes are consumed during reactions.

A calcium ion attaches to an enzyme. This changes the shape of the enzyme so that an organic molecule can enter the enzyme. The calcium ion is a(n)...

An enzyme that removes groups of atoms.

Coenzymes are similar to enzymes for all the reasons except...

What term describes different enzymes that catalyze the same reaction?

Which of these organic processes is not an energy harvesting process?

The most energy is found between the bonds of the ____________ in ATP

The electron transport chain uses what kind of phosphorylation to produce ATP?

Which of these electron carriers is incorrectly matched to its reduced form?

Coenzyme Q is an electron carrier.

Glycolysis is also known as...

Red blood cells do not undergo cellular respiration but instead use another pathway to produce electron carriers. Which of these pathways might red blood cells use?

Select all the steps in cellular respiration in which ATP is produced.

One NADH produces 2 ATP molecules, while one FADH₂ produces 3 ATP molecules

You genetically engineer an obligate anaerobic bacteria to produce superoxide dismutase. What would the new strain be classified as?

Fermentation is a type of anaerobic respiration

An aerotolerant organisms would be able to...

An organism that creates electron acceptors by using an organic electron donor and NO₃^- as an electron acceptor would be classified as...

An organism that uses the Calvin-Benson cycle to fix inorganic carbon would be...

Hydrogen bacteria would be classified as...

An organism that does not use H₂O to reduce CO₂ and sunlight to produce energy from inorganic carbon would be a(n)...

NADPH is found in photoautotrophs

Select all the anoxygenic photoautotrophic organisms on this list.

All organisms use glycolysis

During fermentation, no net NADH are made

Aerotolerant organisms need superoxide dismutase.

The process that removes an amino group from an amino acid is called...

The products of deamination will enter what step to produce ATP?

Lipase _______ _______

The β-Oxidation Pathway prepares what type of biological macromolecule for the electron transport chain