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WorksheetsMicro SU Test 3
Total questions: 70
Worksheet time: 35mins
Which of these best describes why viruses have only certain hosts?
Viruses can only reproduce in a particular type of cell
Viruses can only use a certain type of ribosome to synthesize proteins
Viruses can only attach to a certain cell marker on the host cell
Viruses are destroyed by non host cells
The restriction-modification system includes everything BUT
Host cell receptors
Endonuclease
Methylation
During the lytic cycle, the host cell's DNA is destroyed or disabled.
True
False
An enteric virus would be transmissible via
Respiratory droplets
Fecal-oral route
Skin to skin contact
Sexual contact
Blood transfusions
After an enveloped virus attaches to an animal cell, it then
Inserts its DNA through the cell membrane and into the cytoplasm
Enters the cell via endocytosis and immediately uncoats
Breaks down the cell wall with digestive enzymes
Enters the cell via a vesicle
Which of these would you expect to be made in early mRNA synthesis?
Regulatory proteins
Reverse transcriptase
Capsid proteins
Scaffolding proteins
A temperate phage
Enters the host cell and remains dormant in the cytoplam
Is resistant to environmental factors like heat and pH
Incorporates its genome into the host genome
Causes the host cell to lyse
What would be an indication of a cytocidal infection?
Budding of viral particles from the cell
Presence of hydrolytic enzymes
Integration of viral DNA into the host genome
Reliance on enzymes provided by the host cell
Morphogenesis is
A change in shape of a virus
A change in the spike proteins on the virus' surface
Modifications of a host cell's phenotype due to viral modifcation
The assembly site for virus particles
Antigenic shift is observed in which of these viruses?
Pox virus
Parvo virus
Influenza
HIV
Insect act as ________ when they facilitate viral infection of plants.
Fomites
Vectors
Vehicles
Hosts
All viruses (plant, animal, bacterial) only attach to certain markers on the surface of their host cells.
True
False
Virions only infect plants.
True
False
What is the term for the lipid layer found surrounding some viruses?
Capsid
Glycoprotein
Envelope
Capsomere
A virus that creates disease in the host cell and causes the cell to release virions slowly over time would be described as...
Productive infection with lysis of cells
Productive infection without lysis of cells
Latent infection with lysis of cells
Latent infection with genetic modification of the host cell
A virus in the environment (not inside a cell) would be...
Metabolically active
Metabolically inactive
Catabolic
Anabolic
When a virus infects a bacterial cell, the whole virus enters the cell.
True
False
When would you expect scaffolding proteins to be synthesized?
Late in the infection
Before a virus infects a cell
Early in the infection
The presence of endolysin and holin would indicate what viral replication cycle?
Lytic cycle
Lysogenic cycle
Non-lytic and non-lysogenic reproduction
When sequencing the DNA of a bacterium, you find a segment of code in the chromosome that is not present anywhere in other bacteria of the same species and strain. What is the most likely explanation for how this piece of DNA was introduced into the bacterium's genome?
Transcription errors
Lysogenic infection
Transposition
Conjugation
An organism is deprived of a source of glucose and must switch to fermenting lactose. The gene that codes for lactase has been suppressed by an active repressor. What must happen for the organism to start fermenting lactose?
A corepressor must attach to the active repressor
An inducer must attach to the active repressor
An activator must attach to the activator binding site
An inactive repressor must attach to the active repressor
What mutagen causes an amino group to be changed to a keto group that changes cytosine to uracil?
Nitrous acid
5-bromouracil
2-aminopurine
Intercalating agents
UV light
What type of mutation would be responsible for disrupting protein synthesis?
Resistance mutation
Resistance mutation
Regulatory mutation
rRNA and tRNA mutation
Resistance mutation
What is the primary reason frameshift mutations are so harmful?
They modify the regulatory portion of genes
They create mismatched base pairs
They change all preceding nucleotides
They are not susceptible to photoreactivation
They frequently result in a premature stop codon
Insertion sequences carry genes in addition to those needed for transposition.
True
False
Which of these is NOT a possible effect of transposition
Mutation in coding region
Arrest of translation or transcription
Activate of genes
Generation of new plasmids
T4 lysis
Which enzyme is responsible for proofreading DNA during replication?
DNA ligase
RNA primase
DNA polymerase
Gyrase
Helicase
During excision repair, which enzyme does NOT play an integral role?
Endonuclease
DNA polymerase
DNA ligase
DNA helicase
Direct selection involves growing a population of bacteria on a medium where only the auxotrophic mutants will grow.
True
False
Genes that code for the disease-causing aspects of a bacterium are called
Virulence factors
Pathogenicity islands
Death factors
Vital genes
Select all the horizontal gene transfer methods where the donor cell survives.
Transformation
Transduction
Conjugation
Transmission
Specialized transduction occurs in what kind of infection?
Lytic infection
Lysogenic infection
During conjugation, the bacterial chromosome cannot be transferred, only plasmids.
True
False
What is the likely mechanism of how plasmids accumulate antibiotic resistance genes?
Conjugation
Transposition
Transduction
Transformation
Mobilizable plasmids carry all of the genetic information needed for their transfer during conjugation.
True
False
What is the location where RNA polymerase attaches during transcription?
Promoter
Sigma factor
Hairpin loop
Ribosome
What virus listed can only replicate its genetic information during the S phase of the cell it is infecting?
Pox virus
Parvo virus
HIV
Flu
What kind of bonds are found between complementing bases of DNA?
Phosphodiester bonds
Covalent bonds
Hydrogen bonds
Van der Waal forces
Because most of our genome does not code for protein production, we could lose a lot of our genetic information before feeling negative effects.
True
False
When Oswald Avery injected heat treated smooth and live rough Streptococcus pneumoniae into mice, what happened?
The mice got sick
No change
The mice recovered
Protease is an effective treatment for patients with transmissible spongiform encephalopathy.
False
True
What explains why we have new flu vaccines every year?
Genetic reassortment
Genetic drift
Mutations
Conjugation
Poxviruses have the enzyme that uncoats the capsid and do not need the host enzymes to uncoat.
True
False
In animal viruses, most require at least two different attachment points for the spikes for successful attachment.
True
False
This 20 sided die is most similar to what viral morphology?
Helical
Icosahedral
Complex
Bacteriophage
Pleomorphic
Plant viruses generally do not spread from cell to cell.
True
False
Viroids can be DNA or RNA based.
True
False
A cell's genome is always made of DNA while a virus' genome can be either RNA or DNA
True
False
Only one strand of DNA is picked up by the recipient cell during transformation.
True
False
Competency describes the ability of a cell to perform conjugation.
True
False
Which of the following describes an antiseptic process?
Autoclaving glass pipettes after use
Cleaning a toilet bowl with bleach
Applying antibiotic ointment to a cut
Preparing raw meat separate from vegetables
What is the correct order of most resistant to least resistant to methods of control?
Gram negative bacteria, viruses without envelopes, fungi, naked viruses, protozoa, mycobacteria, endospores
Endospores, mycobacteria, gram negative bacteria, fungi, viruses without envelopes, gram positive bacteria, naked viruses
Naked viruses, gram positive bacteria, viruses without envelopes, fungi, gram negative bacteria, mycobacteria, endospores
Gram positive bacteria, viruses, fungi, mycobacteria, endospores, gram negative bacteria
The a large amount of microbial growth would generally _________ the effectiveness of a method of control.
Inhibit
Enhance
Not change
You want to make your own hand sanitizer. You have some aloe vera gel but must go out to buy the alcohol portion. In the store, there are the following alcohol concentrations available. Which should you use for your homemade hand sanitizer?
95% alcohol solution
50% alcohol solution
15% alcohol solution
70% alcohol solution
Microbes are considered dead when they can no longer reproduce under conditions that would normally support their reproduction.
True
False
What is the D-value of this particular graph?
2 minutes
4 minutes
6 minutes
102.5
100
Most chemical cleaners are more effective at what pH?
<7
neutral pH
>7
Rubbing your hands together while washing with plain water would be a
Chemical method of control
Physical method of control
Both a chemical and physical method of control
Hydrogen peroxide is most effective in its liquid form and on living tissue like a cut or scrape.
True
False
Halogens like iodine work by
Binding to enzymes and disrupting their function
Coagulating proteins and enzymes
Disrupting cell membranes
Oxidizing proteins and other cell components
Anionic detergents like hand soap are considered disinfectants because of the way that they surround hydrophobic molecules and make them soluble in water.
True
False
Ozone is primarily used as a disinfectant for what?
Water
Lab equipment
Food packaging
Surgical rooms
Adding nitrite to a food preserves it by
Inhibiting metabolism
Damaging the cell membrane
Inhibiting Clostridium botulinum endospore germination
Chlorine bleach can destroy all bacteria and fungi, including endospores.
True
False
Phenolics are found in what common household cleaner?
Clorox bleach
Dawn dish soap
Lysol
Drano drain cleaner
Select all chemical methods of control
Heavy metal
Anionic detergents
Ozone
Depth filters
Lyophilization
Autoclaving uses what kind of control method?
Dry heat
Sterilizing gas
UV radiation
Moist heat
Gamma radiation
Freezing kills up to ____ of microbes
25%
90%
50%
10%
In food spoilage, the microbes we are most concerned about are
Acidophiles
Basophiles
Psychrotrophs
Thermophiles
Ionizing radiation is effective against endospores but may or may not kill viruses.
True
False
