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GEMPUR CHAPTER 6: EXPRESSION OF BIOLOGICAL INFORMATION

Total questions: 50

Worksheet time: 27mins

Name
Class
Date
1.
Which do DNA and RNA have in common?
a)
Both are double stranded.
b)
Both contain ribose molecules.
c)
Both contain phosphate groups.
d)
Both contain uracil.
2.

The backbone of polynucleotide is made up of

a)

amino acids and sugars

b)

nucleotide bases, including thymine

c)

nucleotide bases, including uracil

d)

sugar-phosphate molecules

3.

What is the complimentary mRNA sequence to the DNA sequence

5' A-T-T-G-C-A 3'

a)

3' T-A-A-C-G-T 5'

b)

5' U-A-A-C-G-T 3'

c)

3' U-A-A-C-G-U 5'

d)

5' T-A-A-G-C-U 3'

4.
Which defines a codon in DNA or mRNA?
a)
pair of nucleic acid and sugar
b)
pair of phosphate and sugar
c)
three-base code
d)
two-base code
5.
The "central dogma" states that genetic information flows from 
a)
Protein-->mRNA-->DNA
b)
Protein-->DNA-->mRNA
c)
mRNA-->DNA-->Protein
d)
DNA-->mRNA-->Protein
6.
Transcription takes place in the 
a)
cytoplasm
b)
ribosome
c)
mitochondrion
d)
nucleus
7.
The anticodon of a particular tRNA molecule is 
a)
complementary to the corresponding mRNA codon.
b)
complementary to the corresponding triplet in rRNA.
c)
the part of tRNA that bonds with a specific amino acid.
d)
catalytic, making the tRNA a ribozyme.
8.

The region on mRNA that non as non-coding region

a)

introns

b)

exons

c)

codons

d)

anticodons

9.
Translation occurs:
a)
Outside of the Nucleus
b)
Inside of the Nucleus
10.
Translation is crucial to the process of making proteins. Which statement best describes what takes place during translation? 
a)
A copy of chromosomal DNA is created 
b)
Information in mRNA is converted into a sequence of amino acids in a protein 
c)
A RNA copy of a DNA strand is made.
d)
Instructions from DNA in the nucleus are brought to the cytoplasm 
11.
Smaller segments of RNA nucleotides that transport amino acids to the ribosomes.
a)
mRNA
b)
tRNA
c)
rRNA
d)
DNA
12.

Which enzyme joins the Okazaki fragments in the lagging strand?

a)

Helicase

b)

DNA Polymerase

c)

Ligase

d)

Primase

13.

This picture is illustrating the ____ nature of DNA.

a)

antiparallel

b)

semiconservative

c)

helical

d)

radioactive

14.

How do the leading and the lagging strands differ?

a)

The leading strand is synthesized in the same direction as the movement of the replication fork, and the lagging strand is synthesized in the opposite direction.

b)

The leading strand is synthesized at twice the rate of the lagging strand.

c)

The leading strand is synthesized in short fragments that are ultimately stitched together, whereas the lagging strand is synthesized continuously.

d)

The leading strand is synthesized by adding nucleotides to the 3' end of the growing strand, and the lagging strand is synthesized by adding nucleotides to the 5? end.

15.

Which enzyme is responsible for adding the RNA primer to the template strand?

a)

Helicase

b)

DNA Polymerase

c)

Ligase

d)

Primase

16.

What is the result of transcription?

a)

The synthesis of RNA from protein

b)

The synthesis of protein from RNA

c)

The synthesis of protein from DNA

d)

The synthesis of RNA from DNA

17.

What is the result of translation?

a)

The synthesis of RNA from protein

b)

The synthesis of protein from RNA

c)

The synthesis of protein from DNA

d)

The synthesis of RNA from DNA

18.

Which organelle does required in translation?

a)

Nucleus

b)

Ribosome

c)

Mitochondria

d)

Golgi apparatus

19.

What is the role of tRNA?

a)

To treat the DNA strand

b)

To transfer codons

c)

To transfer amino acids

d)

To tackle mutations

20.

What is the start codon?

a)

UAC

b)

TAC

c)

AUG

d)

GUA

21.

How many codons are required for one amino acid?

a)

1

b)

2

c)

3

d)

4

22.

How many codons are required for three amino acids?

a)

1

b)

2

c)

3

d)

4

23.

Which of the following is the major enzyme that involved in DNA

replication?

I. Helicase

II. Reverse transcriptase

III. Topoisomerase

IV. DNA polymerase

V. Aminoacyl-tRNA synthetase

a)

I, III and IV

b)

I, III and V

c)

I,II, III and IV

d)

II, III, IV and V

24.

The following events occur in the replication of DNA.


1. Bonds between complementary bases break

2. Bonds between complementary bases form

3. DNA molecule uncoils

4. Opposite strands separate

5. Sugar-phosphate backbone form

6. Free nucleotides align with complementary nucleotides on each strand


In which order do these events take place?

a)

1 3 6 4 2 5

b)

3 1 4 6 2 5

c)

3 6 1 4 5 2

d)

4 3 1 6 5 2

25.

What is the role of DNA ligase in DNA replication?

a)

Synthesize of short RNA primer by adding RNA nucleotide

b)

Replaces the RNA primer by adding the DNA nucleotide

c)

Join Okazaki fragment at lagging strand forming phosphodiester bond

d)

Unwind the double stranded DNA to become single stranded DNA

26.

In DNA

replication, new DNA nucleotides are added to a growing DNA by…………………

a)

DNA helicase

b)

DNA polymerase

c)

RNA polymerase

d)

RNA primer

27.
Replicate the following strand of DNA: 
AATCATGGA
a)
AATCATGGA
b)
TTAGTACCT
c)
UUAGUACCU
d)
GGATAUCUA
28.
The enzyme that unzips the DNA to prepare for replication
a)
helicase
b)
replicase
c)
polymerase
d)
synthase
29.
The process of DNA replication is called semi conservative because it results in
a)
none of these
b)
Two DNA molecules.  One original molecule and one with two new strands
c)
two exact copies with new strands
d)
two identical molecules, each with 1 original strand and one new.
30.

What enzyme does transcription use?

a)

DNA polymerase

b)

ligase

c)

RNA polymerase

d)

helicase

31.

Which RNA forms part of the ribosomes?

a)

tRNA

b)

mRNA

c)

rRNA

32.
Which of these bases does not exist in RNA?
a)
C
b)
T
c)
U
d)
A
33.
What is occurring in this picture?
a)
Translation
b)
Replication
c)
Translocation
d)
Transcription
34.

Transcribe the following DNA strand: CTG AAT

a)

GAC TTA

b)

CTG AAT

c)

GAC UUA

d)

CUG AAU

35.
How many amino acids are coded for by this sequence of nucleotides:
ATG GGA ACT CCA
a)
4
b)
2
c)
6
d)
12
36.
What would be the tRNA anticodon for the mRNA codon CAG?
a)
GTC
b)
GUC
c)
CUG
d)
CAG
37.
What would happen if we did not have start codons?
a)
Polypeptide chains would continue to grow
b)
Amino acids would bind to tRNA
c)
Protein synthesis wouldn't occur
d)
mRNA would form a double strand
38.
Which of the following occurs first in translation?
a)
The small ribosomal subunit binds to mRNA
b)
The large ribosomal subunit binds to mRNA
c)
tRNA binds to the mRNA
d)
A peptide bond forms between amino acids
39.
What is the correct amino acid sequence for the mRNA code AUGCCAGUAUGA
a)
Met-Pro-Ala-Val
b)
Met-Pro-Val
c)
Tyr-Gly-His
d)
Tyr-Gly-Arg-His
40.
A protein that binds to the operator and blocks the RNA polymerase
a)
regulator
b)
repressor
c)
activator
d)
corepressor
41.
What is the role of operons in prokaryote gene expression?
a)
It makes the genes prokaryotes be turned on
b)
It accounts for the regulation of gene activity in response to the needs of the cells
c)
To make DNA for the gene
d)
To make the gene turn of and off
42.
The lac operon genes only become expressed if:
a)
lactose binds to the repressor
b)
glucose binds to the repressor
c)
lactose binds to the operator
d)
the repressor binds to the operator
43.
Operons are found in the cells of which of the following types of organisms?
a)
prokaryotes (bacteria)
b)
eukaryotes (plant and animals)
c)
animals only
d)
viruses
44.

What is a gene?

a)

A segment of RNA that encodes for a protein

b)

The portion of the chromosome that does not get transcribed

c)

A segment of DNA that encodes for a protein

d)

The collection of your chromosomes

45.

lac operon was proposed by

a)

Meselson and Stahl

b)

Jacob and Monod

c)

Hershey and Chase

d)

Watson and Crick

46.

For a repressible operon to be transcribed, which of the following must be true?

a)

A corepressor must be present.

b)

RNA polymerase and the active repressor must be present.

c)

RNA polymerase must bind to the promoter, and the repressor must be inactive and not blocking the promoter region.

d)

RNA polymerase cannot be present, and the repressor must be inactive.

47.

The sequence where lac repressor binds on operon is called_______

a)

promoter

b)

lac z

c)

operator

d)

lac I

48.

Which of the following is not a structural gene?

a)

lac z

b)

lac y

c)

lac a

d)

lac I

49.

In the lac operon the inducer is

a)

allolactose

b)

glucose

c)

galactose

d)

all of the above

50.

What is 1 in the diagram?

a)

RNA polymerase

b)

Inducer

c)

Repressor

d)

Operator

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