WorksheetsCosmid and cosmid cloning
Total questions: 10
Worksheet time: 5mins
Once cosmids are inside the E.coli cells, they don’t generate phage but are propagated as plasmids.
True
False
Which of the following is the correct method to check whether the DNA has entered into the cell or not in the case of cosmid?
a) If a transformation has taken place turbid plaques are formed
b) If a transformation has taken place clear plaques are formed
c) If a transformation has taken place, it can be confirmed via ampicillin resistance
d) If a transformation has taken place, it can be confirmed if forms plaques and is ampicillin resistant also
Which size of the insert is accepted by the cosmids?
a) 10-20 kbp
b) 35-45 kbp
c) 50-60 kbp
d) 100-120 kbp
Cosmids lack
genes coding for viral proteins
origin of replication
marker genes coding for replication
cleavage site for the insertion of foreign DNA
Cosmid vectors are
phages that lack cos site
plasmids that have no selection marker
plasmids that contain fragment of λ DNA including the cos site
cryptic plasmids
Cosmid vectors are used for
cloning large fragments of DNA
cloning prokaryotic DNA only
cloning eukaryotic DNA only
cloning small fragments of DNA
Cos site of the cosmids
contains cleavage site
helps whole genome in circularization and ligation
Consists of 12 bases
both (2) and (3)
Phagemid consist of
plasmid vector carrying λ phage’s cos site
plasmid vector carrying λ attachment (λ att) site
plasmid vector carrying origin of replication of λ phage only
plasmid vector carrying origin of replication of plasmid only
Which of the following assertions is true in regard to a cosmid vector?
No plaques are produced
Lac-selection system is used
It can carry small DNA fragments
Origin of replication is taken from lambda
If cosmid vector infected cells are grown on an agar plate containing antibiotic, what type of colonies will grow?
Non-recombinants
Recombinants
Both non-recombinants and recombinants
Cells with self-ligated vectors
