wayground logo

Free Printable Worksheets

Font size

S
M
L
XL
Worksheets

OH&E: Tissue Processing

Total questions: 22

Worksheet time: 12mins

Name
Class
Date
1.

The most common "fixative" that is being used throughout in Tissue Processing.

a)

Chloroform

b)

Formalin

c)

Alcohol

d)

Xylene

2.

This tissue processing involves the removal of alcohol from the specimen making way for an easy incorporation of the embedding material that will follow:

a)

Clearing

b)

Embedding

c)

Mounting

d)

Dehydration

3.

This is a process of preserving the tissue specimen from decay and is considered to be the most influential factor in the outcome of the entire process:

a)

Gross examination of tissues

b)

Fixation of tissues

c)

Dehydration of tissues

d)

Rehydration of tissues

4.

These specimen tissue section are often used for "special staining" methods in the laboratory setting.

a)

Step Sections

b)

Microtomy Sections

c)

Serial Sections

d)

Intermediate Sections

5.

Dehydration of tissues often use this particular sequence of either ethanol or isopropyl alcohol in the process:

a)

Ascending order of alcohol concentrations

b)

Descending order of alcohol concentrations

c)

Neutral order of alcohol concentrations

6.

These are substances that will make the microtome sections of the specimen stick to the slides for staining.

a)

Elmer's-GlueR

b)

Albumin

c)

poly-L-lysine

d)

Epoxy resin

e)

All except Epoxy Resin

7.

This method that is widely employed for sectioning tissues.

a)

Paraffin Method

b)

Embedding Method

c)

Fixation Method

d)

Preservation Method

8.

Formalin is a solution containing...

a)

Formaldehyde

b)

Methylene glycol and Formaldehyde

c)

Methylene glycol

d)

Alcohol

9.

The process by which water molecules are removed from the tissue to enable penetration of the embedding material into their space is called...

a)

Embedding

b)

Clearing

c)

Dehydration

d)

Rehydration

10.

Formalin is a solution containing...

a)

Formaldehyde

b)

Methylene glycol and Formaldehyde

c)

Methylene glycol

d)

Alcohol

11.

Normal fixation takes place in three(3) steps: (choose the corrrect order)


I. Methylene glycol quickly penetrates the tissue.

II. Specimen surrounded by fixatives.

III. Formaldehyde binds very slowly to the proteins in the tissue by cross linking.

IV. Some methylene glycol is slowly converted to formaldehyde by dehydration.

a)

IV, III, II, I

b)

I, II, IV

c)

II, IV

d)

I, III, IV

12.

Is a process of cutting serial sections of paraffin-embedded blocks with a tissue microtome.

a)

Microtomy

b)

Sectioning

c)

Slicing

d)

Shredding

13.

The method of obtaining continuous ribbons from a paraffin block and placing sequential sections on successive slides.

a)

Microtome Sectioning

b)

Intermediate Sectioning

c)

Step Sectioning

d)

Serial Sectioning

14.

The Stain that is usually used in paraffin embedded specimen is the...

a)

H & E stain

b)

C & X stain

c)

H & D stain

d)

H & M stain

15.

The method of obtaining continuous ribbons from a paraffin block and placing sequential sections on successive slides.

a)

Microtome Sectioning

b)

Intermediate Sectioning

c)

Step Sectioning

d)

Serial Sectioning

16.

The most commonly used "clearing" agent is _________ and is miscible with both alcohol and paraffin wax.

a)

Formalin

b)

Iodine

c)

Alcohol

d)

Xylene

17.

Formalin is a solution containing...

a)

Formaldehyde

b)

Methylene glycol and Formaldehyde

c)

Methylene glycol

d)

Alcohol

18.

Is the Last step in the making of the light microscopic slide. Cover slips are placed on top of the specimen to better preserve it for a long period of time

a)

Mounting of tissue sections

b)

Staining of Slides

c)

Mounting of cover slip

d)

Embedding

19.

Instruction: Identify the step of tissue processing and its purpose.


Name of step:

Purpose:

a)

Name of step: Dehydration

Purpose:Alcohol replaces water in all cells

b)

Name of step: Clearing

Purpose: Xylene dissolves alcohol

c)

Name of step: Embedding

Purpose: Paraffin displaces xylene. Specimen is now ready to be embedded.

20.

Instruction: Identify the step of tissue processing and its purpose.


Name of step:

Purpose

a)

Name of step: Embedding

Purpose: Paraffin displaces xylene. Specimen is now ready to be embedded.

b)

Name of Step: Dehydration

Purpose: Alcohol replaces water in all cells

c)

Name of step: Clearing Purpose: Xylene dissolves alcohol

21.

Instruction: Identify the step of tissue processing and its purpose.

a)

Name of step: Clearing

Purpose: Xylene dissolves alcohol

b)

Name of step: Embedding

Purpose: Paraffin displaces xylene. Specimen is now ready to be embedded

c)

Name of Step: Dehydration

Purpose: Alcohol replaces water in all cells

22.

The method of obtaining continuous ribbons from a paraffin block and placing sequential sections on successive slides.

a)

Microtome Sectioning

b)

Intermediate Sectioning

c)

Step Sectioning

d)

Serial Sectioning