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WorksheetsOH&E: Tissue Processing
Total questions: 22
Worksheet time: 12mins
The most common "fixative" that is being used throughout in Tissue Processing.
Chloroform
Formalin
Alcohol
Xylene
This tissue processing involves the removal of alcohol from the specimen making way for an easy incorporation of the embedding material that will follow:
Clearing
Embedding
Mounting
Dehydration
This is a process of preserving the tissue specimen from decay and is considered to be the most influential factor in the outcome of the entire process:
Gross examination of tissues
Fixation of tissues
Dehydration of tissues
Rehydration of tissues
These specimen tissue section are often used for "special staining" methods in the laboratory setting.
Step Sections
Microtomy Sections
Serial Sections
Intermediate Sections
Dehydration of tissues often use this particular sequence of either ethanol or isopropyl alcohol in the process:
Ascending order of alcohol concentrations
Descending order of alcohol concentrations
Neutral order of alcohol concentrations
These are substances that will make the microtome sections of the specimen stick to the slides for staining.
Elmer's-GlueR
Albumin
poly-L-lysine
Epoxy resin
All except Epoxy Resin
This method that is widely employed for sectioning tissues.
Paraffin Method
Embedding Method
Fixation Method
Preservation Method
Formalin is a solution containing...
Formaldehyde
Methylene glycol and Formaldehyde
Methylene glycol
Alcohol
The process by which water molecules are removed from the tissue to enable penetration of the embedding material into their space is called...
Embedding
Clearing
Dehydration
Rehydration
Formalin is a solution containing...
Formaldehyde
Methylene glycol and Formaldehyde
Methylene glycol
Alcohol
Normal fixation takes place in three(3) steps: (choose the corrrect order)
I. Methylene glycol quickly penetrates the tissue.
II. Specimen surrounded by fixatives.
III. Formaldehyde binds very slowly to the proteins in the tissue by cross linking.
IV. Some methylene glycol is slowly converted to formaldehyde by dehydration.
IV, III, II, I
I, II, IV
II, IV
I, III, IV
Is a process of cutting serial sections of paraffin-embedded blocks with a tissue microtome.
Microtomy
Sectioning
Slicing
Shredding
The method of obtaining continuous ribbons from a paraffin block and placing sequential sections on successive slides.
Microtome Sectioning
Intermediate Sectioning
Step Sectioning
Serial Sectioning
The Stain that is usually used in paraffin embedded specimen is the...
H & E stain
C & X stain
H & D stain
H & M stain
The method of obtaining continuous ribbons from a paraffin block and placing sequential sections on successive slides.
Microtome Sectioning
Intermediate Sectioning
Step Sectioning
Serial Sectioning
The most commonly used "clearing" agent is _________ and is miscible with both alcohol and paraffin wax.
Formalin
Iodine
Alcohol
Xylene
Formalin is a solution containing...
Formaldehyde
Methylene glycol and Formaldehyde
Methylene glycol
Alcohol
Is the Last step in the making of the light microscopic slide. Cover slips are placed on top of the specimen to better preserve it for a long period of time
Mounting of tissue sections
Staining of Slides
Mounting of cover slip
Embedding
Instruction: Identify the step of tissue processing and its purpose.
Name of step:
Purpose:
Name of step: Dehydration
Purpose:Alcohol replaces water in all cells
Name of step: Clearing
Purpose: Xylene dissolves alcohol
Name of step: Embedding
Purpose: Paraffin displaces xylene. Specimen is now ready to be embedded.
Instruction: Identify the step of tissue processing and its purpose.
Name of step:
Purpose
Name of step: Embedding
Purpose: Paraffin displaces xylene. Specimen is now ready to be embedded.
Name of Step: Dehydration
Purpose: Alcohol replaces water in all cells
Name of step: Clearing Purpose: Xylene dissolves alcohol
Instruction: Identify the step of tissue processing and its purpose.
Name of step: Clearing
Purpose: Xylene dissolves alcohol
Name of step: Embedding
Purpose: Paraffin displaces xylene. Specimen is now ready to be embedded
Name of Step: Dehydration
Purpose: Alcohol replaces water in all cells
The method of obtaining continuous ribbons from a paraffin block and placing sequential sections on successive slides.
Microtome Sectioning
Intermediate Sectioning
Step Sectioning
Serial Sectioning
