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Worksheetsenzyme engineering
Total questions: 25
Worksheet time: 15mins
Which is the most correct statement regarding enzymes?
Enzymes are smaller than its substrate.
Extreme pH or temperature can damage enzymes.
All proteins are enzymes.
Enzymes increase the activation energy of a reaction.
Function of enzyme in human body.
Digestion of food
Destroying toxins
Building muscles
All of the above
Which one of the following is not a method used in enzyme engineering?
Rational design
Acceleration evolution
Directed evolution
None of the above
Principle of Rational design.
Darwinian evolution
Random mutation
Site specific mutagenesis
None of the above
Choose the correct statement regarding Directed evolution.
Mutation created at a defined site in a DNA molecule.
Detailed knowledge of structure and function of protein is required.
Most efficient mutated enzyme is selected after screening.
Mutagenic primer added at a specific site.
Use of enzyme engineering.
To produce enzyme in large quantity
To alter specificity for broader substrate
Both a and b
None of the above
Advantage of Rational design.
Structural knowledge of an enzyme is not needed.
Technically easy
Inexpensive
All of the above
Match the following
A-ii, B-iv, A-iii, B-i
A-ii, B-iii, A-i, B-iv
A-iv, B-iii, A-i, B-ii
A-i, B-ii, A-iii, B-iv
A combination of Y37C and Q195C mutations applied to TyrRS. What will be the result?
Its specificity towards L-tyrosine increased.
It can recognize 3-iodo-L-tyrosine rather than L-tyrosine.
It cannot recognize both 3-iodo-L-tyrosine and L-tyrosine.
It can recognize L-tyrosine rather than 8-iodo-L-tyrosine.
Which of the following is the correct order of steps in enzyme engineering?
Introducing to host cell > Gene construction > Molecular modeling > Isolation of native enzyme > Analyzed with database > Isolation of mutant enzyme.
Isolation of native enzyme > Analyzed with database > Molecular modeling > Gene construction > Introducing to host cell > Isolation of mutant enzyme.
Gene construction > Analyzed with database > isolation of native enzyme > Molecular modelling > Introducing to host cell > Isolation of mutant enzyme.
Introducing to host cell > Molecular modelling > Gene construction > Isolation of native enzyme > Analyzed with database > Isolation of mutant enzyme.
Choose the correct option that define enzyme engineering.
Substrate binds to the active site of an enzyme and forms products.
It is the process of converting substrates into products using enzymes.
It is the production of new proteins using enzymes.
It is the process of designing enzyme by changing the sequence of amino acids.
What will be the result, when 3 disulfide bonds introduce at 142-21, 164-9, 97-3 sites of T4lysozyme?
Stability of the enzyme increases.
Melting temperature increases.
Melting temperature decreases.
Activity increases.
Which of the properties of enzyme that may change during enzyme engineering?
Thermostability
Molecular weight
Kinetic property
All of the above
Principle of Directed evolution.
Site specific mutation
Random mutation
Both a and b
None of the above
Out of the following which is an industrial application of enzyme engineering?
Production of brome lain
Production of protein vaccines
Diagnostic reagent
None of the above
Binding proteins of non-Ig origin developed using combinatorial protein engineering techniques are called ----.
Protein vaccines
Insertional proteins
Zinc finger proteins
Affibody binding proteins
Observe the given table. Which is the most suitable statement about the trend of % activity and Tm ?
Both Tm and % activity decreasing.
Greater the number of disulfide bond, greater is its % activity.
Tm increases and % activity decreases with increase in number of disulfide bond.
% activity is directly proportional to number of disulfide bond.
After how many rounds of evolution process, the mutant GpdQ enzyme shows the enhanced activity towards BpNPP?
4
8
10
14
Which is the method used to enhance the activity of GpdQ enzyme?
Directed evolution
Semi rational design
Rational design
none of the above
Which properties are tested in DE to select most efficient mutant enzyme?
Stability, Selectivity
Affinity, Activity
Solubility, Basicity
Availability, Melting point
Observe the flowchart and figure out the method.
Rational design
Semi rational design
Directed evolution
None of the above
Which of the following application of enzyme engineering is not used in medical field?
Therapeutic antibodies
Glucose isomerase
Interferon's
Protein vaccines
Affibody binding proteins have high affinity. So it can be used in -------
Diagnostics
Viral targeting
Bioseperation
All of the above
Which type of PCR methods are used in GpdQ enzyme engineering?
epPCR
Assembly PCR
Alu PCR
StEP PCR
What type of mutation is applied in enzyme engineering?
Addition of amino acids
Deletion of amino acids
Substitution of amino acids
All of the above
