NEW
Font size
WorksheetsRevision - Chromatography
Total questions: 35
Worksheet time: 18mins
A hydrophobic compound will preferentially partition into an aqueous solvent. True or false?
True
False
Chromatography is a physical method that is used to separate and analyse _____________.
Pure compounds
Complex mixtures
Viscous mixtures
Metals
The process of passing a mobile phase through a chromatography column is called which one of the following?
Flushing
Washing
Elution
Partitioning
In which type of chromatography, the stationary phase held in a narrow glass tube and the mobile phase is forced through it under pressure?
Column chromatography
Planar chromatography
Liquid chromatography
Gas chromatography
In chromatography, which of the following can the mobile phase be made of?
Solid or liquid
Liquid or gas
Gas only
Liquid only
A thin layer chromatography (TLC) plate was set up with a non-polar solvent, hexane, and a polar stationary phase, silica gel. The chromatogram below was obtained. A ruler was then placed next to the plate. Calcualte the Rf value for the compound.
0.80
0.78
0.64
0.61
Which of the following types of chromatography involves the process, where the mobile phase moves through the stationary phase by the influence of gravity or capillary action?
Column Chromatography
High Performance Liquid Chromatography
Gas Chromatography
Planar Chromatography
Which of the following cannot be used as an adsorbent in Column adsorption chromatography?
Magnesium oxide
Silica gel
Activated alumina
Potassium permanganate
In Gas-liquid phase chromatography, the stationary phase is composed of _________ and the mobile phase is made of _________
Solid, liquid
Liquid, liquid
Liquid, gas
Solid, gas
Four straight chain alchohols, S, T, U, V, with a general formula ROH, were analysed using a gas chromatograph combined with a mass spectrometer. The following chromatogram was produced.
V, U, T, S
T, U, S, V
V, S, U, T
S, T, U, V
The pH at which a protein carries a net zero charge is termed which of the following?
pKa
pKb
pI
K
SDS-PAGE separates molecules on the basis of which of the following characteristics?
Electrical charge
Size
Three-dimensional shape
Isoelectric point (pI)
What is the first stage of the two-stage two-dimensional PAGE?
SDS-PAGE
HPLC
Isoelectric focussing
Sedimentation
Spectroscopy measures the change in behaviour of a molecule when it is exposed to which of the following?
A centrifugal force
Electromagnetic radiation
An electrical charge
Acidic conditions
A combination of paper chromatography and electrophoresis involves ______________.
partition chromatography
electrical mobility of the ionic species
both (a) and (b)
none of these
Thin layer chromatography is
partition chromatography
electrical mobility of ionic species
adsorption chromatography
none of the above
In gas chromatography, the basis for separation of the components of the volatile material is the difference in
partition coefficients
conductivity
molecular weight
molarity
In reverse phase chromatography, the stationary phase is made
non-polar
polar
either non-polar or polar
none of these
Ion exchange chromatography is based on the
electrostatic attraction
electrical mobility of ionic species
adsorption chromatography
partition chromatography
HPLC stands for
High Pressure Liquid Chromatography
High Performance Liquid Chromatography
both (a) and (b)
Highly Placed Liquid Chromatography
The eluent strength is a measure of
solvent adsorption energy
solvent absorption energy
solvent diffusivity
solvent mixing index
HPLC methods include
liquid/liquid (partition) chromatography
liquid/solid (adsorption) chromatography
ion exchange and size exclusion chromatography
all of the above
Column efficiency is measured in terms of number of plates which is
inversely related to the square of the peak width
directly related to the square of the peak width
inversely related to the cube root of the peak width
directly related to the square of the peak width
An isocratic elution in HPLC is one in which the composition of the solvent
remains constant
changes continuously
changes in a series of steps
none of these
In reversed phase HPLC, there is a
non polar solvent/polar column
polar solvent/non-polar column
non polar solvent/non-polar column
any of the above
A gradient elution in HPLC is one in which the composition of the solvent
remains constant
is changed continuously or in a series of steps
both (a) and (b)
none of the above
In normal phase HPLC, there is a
non polar solvent/polar column
polar solvent/non-polar column
non polar solvent/non-polar column
any of the above
Which can be used as a mobile phase in HPLC applications?
Any compound with solubility in liquid
Any compound with limited solubility in liquid
Any compound with non-solubility in liquid
Any of the above
Which of the following is not used for detection in GC?
Infrared spectroscopy
NMR
Flame ionisation
Electrical conductivity
The GC trace obtained after an experiment is called a
chromatograph
chromatogram
chromatophore
graph
Which of the following detectors give concentration-dependent signals?
Electron-capture detector
Thermal conductivity
Infra-red detector
All of these
Which of the statements is correct?
Gas chromatography is used to analyse gases
Gas chromatography is used to analyse solids
Gas chromatography is used to analyse gases, solutions and solids
All of the above
Helium is generally preferred as carrier gas over nitrogen and hydrogen because
it is inert
it has a lower viscosity
it doubles up as a party gas for balloons and funny voices
all of above
What are the benefits of decreasing the column internal diameter?
Increased sample capacity
Increased resolution
Reduced risk of column overloading
All of the above
Which of the following gases is unsuitable for use as a GC carrier gas?
Nitrogen
Helium
Oxygen
All of the above
