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WorksheetsMethod in gene cloning
Total questions: 10
Worksheet time: 3mins
First stage involved in gene cloning.
Amplification of the target gene
Insertion of the target gene
Polymerase Chain Reaction
Isolation of the target gene
The target gene can only be detected by DNA probes.
True
False
The plasmids carrying the target gene (recombinant plasmids) must be introduced into a host cell through _____.
Cloning
insertion
Transformation
Isolation
Polymerase Chain Reaction begins with _______.
extension
annealing
replication
denaturation
The tools required for (PCR) excepts
Taq polymerase
RNA polymerase
Thermocycler
dNTPs
PCR is a method that produce many copies of DNA in vitro.
True
False
There are two types of bacteria or host cell that results from the introduction of the host cells.
True
False
Types of plasmids produced after insertion of the target gene into a vector.
Recombinant plasmids
Non-recombinant plasmids
Bacterial plasmids
Isolated plasmids
Gel that used in gel electrophoresis process.
Agarose gel
Aloe Vera gel
Polymer Rubber gel
Vaseline gel
In reverse transcription process, DNA acts as template
True
False
