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Worksheets[Revision] Centrifugation
Total questions: 27
Worksheet time: 14mins
How might solid sodium carbonate be obtained from sodium carbonate solution?
Centrifugation
Filtration
Evaporation
It cannot be extracted
After centrifugation when sublimate settles, clear liquid
can be allowed to rest
can be allowed to form crystals
can be decanted off
can be evaporated
After centrifugation, sublimate
dissolves completely
remain suspended in a liquid
settles at bottom
depends upon pH of sublimate
Which of the following is an effective way of purifying liquids containing suspensions?
crystallization
decanting
separating funnel
centrifuging
Fine insoluble solid particles can be removed through which of the following process?
crystallization
decanting
centrifuging
separating funnel
Process quicker than filtration but not so effective is
decanting
centrifuging
crystallization
fractional distillation
Differential centrifugation relies on the differences in ______ of biological particles of different
Size, density
Sedimentation rate, sizes and density
Size, structure
Mass, size
Which of the following statements about the basic principle of sedimentation is False?
The denser a biological structure is, the faster it sediments in a centrifugal field
The more massive a biological particle is, the slower it moves in a centrifugal field
The denser the buffer system is, the slower the particle will move in a centrifugal field
The greater the centrifugal force is, the faster the particle sediments
Which of the following is/are the application of analytical centrifugation?
I. The determination of the purity of macromolecules.
II. The determination of the relative molecular masses of solutes in their native state.
III. The examination of changes in the molecular mass of supramolecular complexes.
IV. The detection of conformational changes.
I only
I and II only
I, II and III only
I, II, III and IV
Which of the following is/are appropriate care and maintenance of centrifuges?
I. Proper balancing of centrifuge tubes.
II. Avoid long time exposure of the rotors to alkaline solution and acidic buffers.
III. Cleaning centrifuges and rotors with coarse brushes and then wash them with distilled or de-ionized water after every run.
IV. For overnight storage, the rotors are left upside down and then positioned in a safe and dry place.
I, II, III only
II, III and IV only
I, II, and IV only
II only
After centrifugation of milk, the supernatant is?
Fat
Whey
Casein
Water
What is the name of the machine that spins in order to separate out components making up a mixture?
Centrifuge
Blood plasma
Centrifugation
Centrifugation tube
How does centrifugation work?
Through dripping particles
Through spinning
By keeping large particle in the center and smaller on the outside
By separating particles into different tubes
If you were centrifuging whole milk, which would you expect to get flung further out from the center of the centrifuge?
The skim milk
The cream/fat
Both would be equally flung
Neither would be flung
Which of the following scientist invented the centrifugal separator?
Gustaf de Laval
Donald ronholm
Luis pastuer
Rutherford
The skimming efficiency of a separator consequently decreases with increased throughput.
True
False
The centrifugation is based on the principle of when a force is less than the gravity desired.
True
False
In 500 × g, what does g represent in accordance to centrifugation?
Gravitational force
Centrifugal force is 500 times greater than earthly gravitational force
Centrifugal force is 500 times less than earthly gravitational force
Centrifugal force is 500 times same as that of earthly gravitational force
Which of the following is not a type of centrifugation?
Hydro cyclone
Tubular centrifuge
Microfiltration
Disk stack separator
By increasing the feed rate of the liquid in tubular centrifuge the performance is increased.
True
False
At what speed do you centrifuge blood?
2200-2500 RPM
3000-3200 RPM
1000-1500 RPM
4000 RPM
Which of the following centrifugation is used to separate certain organelles from whole cell?
Rate-zonal centrifugation
Normal centrifugation
Differential centrifugation
Isopycnic centrifugation
What is rate-zonal centrifugation?
Based on separation of particles by mass
Based on separation of particles by density
Based on separation of particles on solubility
Based on separation of particles on size
Differential centrifugation is based on the differences in ______ of biological particles of different ___________.
Size, density.
Sedimentation rate, sizes and density.
Size, structure.
Mass, size.
Which of the following statements about the basic principle of sedimentation is False?
The denser a biological structure is, the faster it sediments in a centrifugal field.
The more massive a biological particle is, the slower it moves in a centrifugal field.
The denser the buffer system is, the slower the particle will move in a centrifugal field.
The greater the centrifugal force is, the faster the particle sediments.
Which of the following is/are the application of analytical centrifugation?
i. the determination of the purity of macromolecules.
ii. the determination of the relative molecular masses of solutes in their native state.
iii. the examination of changes in the molecular mass of supramolecular complexes.
iv. the detection of conformational changes.
i only.
i and ii only.
i, ii, and iii only.
All of these.
Which of the following is/are appropriate care and maintenance of centrifuges?
i. Proper balancing of centrifuge tubes.
ii. Avoid long time exposure of the rotors to alkaline solution and acidic buffers.
iii. Cleaning centrifuges and rotors with coarse brushes and then wash them with distilled or deionised water after every run.
iv. For overnight storage, the rotors are left upside down and then positioned in a safe and dry place.
i, ii, iii only.
ii, iii and iv only.
i, ii, and iv only.
All of these
