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WorksheetsBIOT-625
Total questions: 20
Worksheet time: 20mins
Which of the following spectroscopy techniques is associated with molecular emission?
UV-Visible spectroscopy
IR spectroscopy
Fluorescence spectroscopy
X-ray diffraction
Separation of ions in mass spectrometer take place on the basis of which of the following?
Mass
Charge
Molecular weight
Mass to charge ratio
Identify the correct statement
NMR signals towards the left of the spectrum correspond to low chemical shift values
NMR signals towards the right of the spectrum correspond to high chemical shift values
Chemical shift values are larger when shielding effects are greater
Chemical shift values are larger when deshielding effects are greater
The Excitation and Emission spectra are the prime necessity of selection of visualization dye in
NMR spectra
Mass spectra
Fluorescence spectra
X-ray diffraction
A UV/Vis spectrophotometer may be used as a detector for:
Gas Liquid chromatography
Chromatography
High Performance liquid chromatography
Size exclusion chromatography
Fractional precipitation of proteins is achieved by
Sodium chloride
Ammonium sulfate
Ammonium per sulfate
Tri sodium citrate
Desalination and demineralization can be achieved by
Reverse osmosis
Nanofiltration
ultrafiltration
microfiltration
Sodium Dodecyl Sulphate (SDS) is used while separating proteins by polyacrylamide gel electrophoresis because
It helps in solubilization of proteins thereby making it easier to separate
It binds to proteins and confers uniform negative charge density thereby making them move during electrophoresis
Decreases the surface tension of the buffer used for electrophoresis
Stabilizes the proteins
Identify the enzyme not used for the cell lysis
Cellulase
Lyticase
lysozyme
Manase
In stacking gel the electrophoretic mobility order is:
glycinate ions > Cl- > proteins
C l- > proteins > glycinate ions
C l- > proteins SDS complex > glycinate ions
C l- > proteins SDS complex > glycine.
Select true or false
a. β-mercaptoethanol is a cross linking agent
b. Lauryl sulfate is used for providing density
c. Bis acrylamide is a reducing agent
d. Glycerol is an anionic detergent
all true
all false
a and c are true
b and c are true.
“The pH at which the net charge of the protein is zero and its movement stops.” Is the principle of:
immuno electrophoresis
capillary electrophoresis
Native PAGE
iso electrofocusing
Which type of microscope uses an Annular stop
TEM
SEM
Phase contrast microscope
Atomic force microscope
Identify one difference between SEM and TEM sample preparation
Only SEM requires Sputter coating
Only TEM requires Sputter coating
Only SEM requires dehydrating
Only TEM requires dehydrating
Which of the following techniques would be most useful to identify and quantify the presence of a known impurity in a drug substance?
NMR
IR
MS
HPLC
Criteria required for NMR signal
Nuclei should have spin quantum no. (I=1/2)
External magnetic field should be applied
Energy should be supplied in the form of EMR
All the above
Name the instrument that has three major components as:
1. Ion source
2. Analyzer
3. Detector system
for identification of unknown compounds, structure elucidation and determination of chemical properties.
NMR spectrometry
HPLC
Mass Spectrometry
Fluorescence spectrometer
Which of the following is used as a carrier gas in gas chromatography?
Carbon dioxide
Helium
Oxygen
Methane
Ion exchange chromatography is based on?
Electrostatic attraction
Partition chromatography
Adsorption chromatography
Electrical mobility of ionic species
In size exclusion chromatography, solute molecules are separated based on _________
Molecular composition
Molecular geometry and size
Molecular phase
Molecular formula
