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Revision Chapter 1 and 2 (Microbiology and Histology)

Total questions: 50

Worksheet time: 25mins

Name
Class
Date
1.

These bacteria are

a)

gram positive

b)

gram negative

c)

nonliving

d)

viral

2.

These bacteria are

a)

gram positive and negative

b)

gram positive

c)

gram negative

d)

viral

3.

Which is applied first in gram staining?

a)

alcohol

b)

crystal violet

c)

grams iodine

d)

safranin

4.

Which is applied last in gram staining?

a)

alcohol

b)

safranin

c)

crystal violet

d)

grams iodine

5.

Which layer of the bacteria is dyed/stained purple?

a)

Cell membrane

b)

cell wall

c)

capsule

d)

slime layer

6.

Choose the correct steps in tissue processing.

a)

Infiltration with paraffin- Clearing- Dehydration-Fixation

b)

Fixation- Clearing- Dehydration- Infiltration with paraffin

c)

Fixation-Dehydration-Clearing- Infiltration with paraffin

d)

Infiltration with paraffin- Fixation- Clearing- Dehydration

7.

The objective of fixation is to prevent the cell from changing.

a)

True

b)

False

8.

What is histology?

a)

Study of organs

b)

Study of different types of tissue

c)

Study of histamines

d)

Study of skin

9.

Thick peptidoglycan in cell wall

a)

Gram positive bacteria

b)

Gram negative bacteria

10.

Thin peptidoglycan in cell wall

a)

Gram positive bacteria

b)

Gram negative bacteria

11.

Gram positive bacteria will be stained in __________ color after Gram staining procedure?

a)

Red

b)

Pink

c)

Purple

d)

Blue

12.

Which of the following bacteria is Gram negative bacteria?

a)

Staphylococcus aureus

b)

Streptococcus pneumoniae

c)

Escherichia coli

d)

Staphylococcus epidermidis

13.

Which of the listed dyes is the basic dye?

a)

Eosin

b)

Romanowsky dye

c)

Hematoxylin

14.

What is the coloring agent of hematoxylin?

(a)  

15.
What is the primary purpose of keeping doors and windows closed during the laboratory session?
a)
To reduce the incidence of contamination from microbes traveling in the air.
b)
To keep you all in the room.
c)
To increase the incidence of contamination from microbes in the room.
16.
After using an inoculating loop or needle to transfer a culture from one medium to the next, what stop should be taken next to avoid contamination.
a)
Heat the inoculating loop or needle with the bunsen burner flame until it becomes cool to the touch.
b)
Heat the inoculating loop or needle with the bunsen burner flame until it becomes purple hot.
c)
Heat the inoculating loop or needle with the bunsen burner flame until it becomes red hot.
17.
When should lab bench tops be cleaned with disinfectant solution?
a)
Never, take a chance!
b)
At the beginning and end of lab.
c)
Only at the end.
18.
It is especially important to use rapid and efficient manipulation to prevent reproductive spore dissemination when handling which type of culture?
a)
bacterial
b)
fungal
c)
viral
19.
If you happen to spill a bacterial culture on the floor during lab, which of the following statements describes the correct clean-up procedure?
a)
Immediately cover the spill with paper towels, saturate with disinfectant, then let sit for 20 minutes.
b)
Immediately cover the spill with paper towels, saturate with water, then let sit for 20 minutes.
c)
Immediately cover the spill with paper towels, saturate with disinfectant, then walk away.
20.
It is very important that microbiology students must develop aseptic techniques in order to prepare pure cultures. which of the following statements properly defines aseptic technique?
a)
A technique that is full of contaminating organisms
b)
A way to make you get sick when you want to!
c)
A technique that is free from contaminating organisms
21.
Which of the following is an example of proper laboratory technique?
a)
Do not wear disposable gloves when working with blood, serum, and other body fluids.
b)
Wear disposable gloves when working with blood, serum, and other body fluids.
22.
What should be done with a paper towel used to cover a spilled microorganism after it has been soaked with disinfectant?
a)
Place the disinfected paper towels into a trash can.
b)
Place the disinfected paper towels into a biohazard bag.
23.
How are microorganisms destroyed after they replaced into a biohazard bag?
a)
They are thrown away and hope for the best.
b)
Microorganisms are destroyed by placing the biohazard bag into an autoclave and killing them with steam pressure.
24.

A series of techniques designed to avoid contamination.

a)

Culturing technique

b)

Inoculation

c)

Decontamination technique

d)

Sterile technique

25.

The accidental introduction of a microorganism into culture

a)

Contamination

b)

Inoculation

c)

Culturing

d)

Incubating

26.

A liquid medium is called a(n):

a)

Agar

b)

Sterile medium

c)

Biochemical medium

d)

Broth

27.

What is the purpose of an agar?

a)

It prevents contamination

b)

It provides the ingredients needed to observe a biochemical reaction

c)

It is used to solidify a broth

d)

It inhibits the growth of certain organisms

28.

A population of microbes is considered a(n):

a)

A simple stain

b)

A species

c)

A culture

d)

An agar

29.

The staining process that uses more than one chemical stain in order to differentiate between organisms or structures:

a)

Simple stain

b)

Differential stain

c)

Negative stain

d)

Cultural stain

30.
The purpose of streaking a plate is to obtain 
a)
large quantities of bacteria
b)
bacteria in pure culture
31.
When streaking a plate, primarily
a)
flex and extend the fingers
b)
move from the wrist 
c)
move your whole arm
32.
Plates should be incubated
a)
agar side up
b)
agar side down 
c)
it doesn't matter
33.
When streaking a plate, the loop should be flamed
a)
At the beginning and end 
b)
 At the beginning and end and between the first 2 zones 
c)
At the beginning and end and between the second and third zone 
d)
At the beginning and end and between each zone 
34.
George streaks a plate of medium from a broth culture.  After incubation, he sees confluent growth in all sectors.  Which of the following errors could NOT explain this result?
a)
He forgot to pick a colony from the plate
b)
 He forgot to flame the loop between sectors
c)
He picked additional culture from the plate after the first sector
d)
He crossed over the previous streak too many times at the beginning of a new sector.
35.
Which finger should you use to remove the lid from a test tube when you are using an inoculating loop to make a transfer? 
a)
little finger of the dominant hand (hand holding the loop)
b)
little finger of the non-dominant hand (hand holding the sample)
c)
forefinger of the dominant hand (hand holding the loop)
d)
thumb and forefinger of the non-dominant hand (hand holding the sample)
36.
The bacteriology loop should be sterilized in what part of the Bunsen burner flame? 
a)
in the bright blue cone
b)
just above the bright blue cone
c)
above the flame
d)
at the base of the flame
37.
The loop should be held 
a)
straight up and down
b)
at a very slight angle
c)
at a fairly steep angle
d)
parallel to the work bench
38.
When transferring liquid using a pipette, the tip of the pipette should 
a)
be in the center of the test tube
b)
should touch the side of the test tube 
c)
never touch the side of the test tube
d)
remain in the air above the tube and never enter the tube.
39.
When transferring liquid aseptically from one tube to another, you should
a)
remove the cap of the tube with the little finger of the hand holding the pipette
b)
remove the cap of the tube with the little finger of the hand holding the tube
c)
place the cap on the bench top with the inside up
d)
place the cap on the bench top with the inside down
40.

What is the difference between nutrient agar and lactose broth?

a)

Nutrient agar is solid and lactose broth is liquid

b)

Both are liquid but lactose broth has added lactose sugar

c)

Nutrient agar is a selective media and lactose broth is a derived media

d)

there is no difference between the two types

41.

What is the bet pH for most bacterial growth?

a)

2

b)

4

c)

7

d)

11

42.

If you want to select for a specific type of bacteria, what type of media would you use?

a)

Nutrient broth

b)

Differential media

c)

Selective media

d)

an autoclave

43.

Nutrient broth is turned into nutrient agar by the addition of what?

a)

lactose

b)

eggs

c)

agar

d)

peptone

44.

The type of culture media that can turn bacteria different colors on a petri dish is called a ____ media

a)

selective

b)

differential

c)

natural

d)

broth

45.

Regardless of the media type, the best temperature for most bacteria is

a)

10 -15o C

b)

25 - 40oC

c)

60oC

d)

100oC

46.

This type of complex media is more rich than nutrient agar and includes the nutrient hemin.

a)

chocolate agar

b)

blood agar

c)

LB agar

d)

MacConkey agar

47.

This type of media is composed of precise amounts of pure chemicals. It is essential when studying nutritional requirements of bacteria.

a)

selective media

b)

differential media

c)

chemically defined media

d)

enrichment culture media

48.

This type of media inhibits the growth of organisms other than the one being grown. Can be complex or chemically defined media.

a)

selective media

b)

diferential media

c)

chemically defined media

d)

enrichment culture media

49.

This type of media contains specific ingredients that help us to visually distinguish which species do or do not carry out a specific biochemical process.

a)

selective media

b)

differential media

c)

chemically defined media

d)

enrichment culture media

50.

This type of media enhances the growth of one particular species in a mixed population.

a)

selective media

b)

differential media

c)

chemically defined media

d)

enrichment culture media

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