wayground logo

Free Printable Worksheets

Font size

S
M
L
XL
Worksheets

Enzyme Quiz

Total questions: 45

Worksheet time: 40mins

Name
Class
Date
1.

Based on the graph, what temperature does this enzyme work optimally?

a)

15 C

b)

40 C

c)

30 C

d)

47 C

2.
Letter D...
a)
active site
b)
enzyme
c)
substrate
d)
products
3.
Letter B...
a)
active site
b)
enzyme
c)
substrate
d)
products
4.

Enzyme A works optimally at pH 7, however, it was removed from its environment and placed into a pH of 1. This is not in its working range. This enzyme is not nonfunctioning how it used too. What do we call the process of an enzyme becoming not functional due to pH change or temp change?

a)

Naturing

b)

Denaturing

c)

Transcription

d)

Translation

5.

Enzyme A works optimally at pH 7, however, it was removed from its environment and placed into a pH of 1. This is not in its working range. This enzyme is not functioning how it used too. Why is the enzyme not functional anymore?

a)

The enzyme shape has altered

b)

The enzyme changed color

c)

The enzyme used all the substrate

d)

The substrate change shape

6.

One morning, Drake ate lots of salt and vinegar chips, which have lots of acid on them. Throughout the day, Drake was feeling stomach pains and his food in his stomach is not being broken down by the enzymes. Why would the enzymes in Drakes stomach not break down the food?

a)

The enzymes have denatured due to the acid in the chips changing the shape of the enzyme.

b)

The enzymes in the stomach have broken the substrate down in to smaller parts

c)

The enzymes in the stomach have built the pieces of substrate in to larger molecules called polymers

d)

The enzymes will function normally not matter the pH change.

7.

What temperature will Pepsin, in the graph, be the least functional?

a)

40 degrees Celsius

b)

30 degrees Celsius

c)

60 degrees Celsius

d)

90 degrees Celsius

8.

Kristina is studying enzymes and noticed that the protein. The protein in question is shown and has a specific shape. Kristina is manipulating this protein by dropping the temperature to out of the protein's working range. What would be Kristina's observation be after dropping the temperature of the Hemoglobin protein to freezing (out of optimal range) be? SELECT ALL THAT APPLY

a)

The protein denatured and changed shape

b)

The protein denatured and does not function the same

c)

The protein's polypeptide sequence is getting longer

d)

Translation occurs before transcription

9.

Why are there many codon options for the amino acid Proline (Pro)?

a)

this allows for less changes in the polypeptide sequence, resulting the same protein and less mutations if the polymerase was to and a mistake

b)

this allows for more changes in the polypeptide sequence, resulting the same protein and less mutations if the polymerase was to and a mistake

c)

So that the mRNA can fit in the ribosome and be read three RNA bases at a time

d)

So that there are not different triglyceride produced

10.

Lactase is an enzyme that breaks down a sugar found in dairy products known as lactose. Some people are lactose intolerant, and this can be due to not having enough lactase production. People who are lactose intolerant may not feel well after eating foods containing lactose.


What does lactase do to lactose?

a)

break down the bigger sugar, lactose, into monosaccharides

b)

break down the bigger sugar, lactose, into lipids

c)

Combine the monosaccharides to form the bigger sugar, lactose

d)

Combine the nucleic acids to form the bigger sugar, lactose

11.

Lactase is an enzyme that breaks down a sugar found in dairy products known as lactose. Some people are lactose intolerant, and this can be due to not having enough lactase production. People who are lactose intolerant may not feel well after eating foods containing lactose.


What is the substrate in the scenario?

a)

lactase

b)

lactose

c)

enzyme

d)

protein

12.

Lactase is an enzyme that breaks down a sugar found in dairy products known as lactose. Some people are lactose intolerant, and this can be due to not having enough lactase production. People who are lactose intolerant may not feel well after eating foods containing lactose.


If the person that ate the dairy/lactose was to have a fever of 104.3 degrees fahrenheit. What would occur to the protein if this is out of the optimal range of operation? SELECT ALL THAT APPLY

a)

the enzyme, lactase, will denature

b)

the enzyme, lactase, will change structure

c)

the enzyme, lactase, will function less - if at all

d)

the enzyme will break more lactose down

e)

the enzyme will break apart into amino acids

13.

An enzyme called glucocerebrosidase breaks down a glycolipid in the body known as glucocerebroside. However, in a genetic disease known as Gaucher’s disease, the body does not produce enough glucocerebrosidase. Therefore glucocerebroside can build up in the body and this can cause serious side effects such as anemia and swelling of the liver and spleen.

Where do instructions to synthesize glucocerebrosidase originate from?

a)

DNA

b)

mRNA

c)

tRNA

d)

Amino Acids

14.

An enzyme called glucocerebrosidase breaks down a glycolipid in the body known as glucocerebroside. However, in a genetic disease known as Gaucher’s disease, the body does not produce enough glucocerebrosidase. Therefore glucocerebroside can build up in the body and this can cause serious side effects such as anemia and swelling of the liver and spleen.

What is the substrate in the scenario?

a)

a glycolipid

b)

a protein

c)

the enzyme

d)

spleen

15.

A popular lab that can be performed by students is to test the reaction rate of catalase enzyme when it acts on the substrate hydrogen peroxide. Catalase has the ability to break down hydrogen peroxide. Catalase can be found in beef liver from the grocery store! However, if the beef liver is boiled first, the catalase will not be able to break down hydrogen peroxide.

What occured to the enzyme in the beef liver, catalase?

a)

it was denatured due to being heated

b)

it was denatured due to being in acidic conditions

c)

it was catalyzed due to the basic conditions

d)

it was denatured due to the non optimal pH conditions

16.

ATP is produced by cellular respiration in your human body cells. There are a variety of enzymes that work to produce ATP, but one of those enzymes is called phosphofructokinase-1. This enzyme is sensitive to blood acidity. Blood can become more acidic if a patient is in respiratory distress.


If you were under respiratory distress, what would be occurring to the phosphofructokinase-1?

a)

It will change shape and function less if at all.

b)

It will NOT change shape, but it WILL change function

c)

It will change shape, but it will NOT change function

d)

it will NOT change shape, and NOT change function

17.

What is the optimal working condition for Salivary Amylase?

a)

6

b)

1

c)

5

d)

10

e)

9

18.

Which two enzymes share a working pH of 7.5?

a)

Pepsin and Salivary Amylase

b)

Alkaline Phosphatase and Pepsin

c)

Pepsin only works at a pH of 6

d)

Salivary Amylase and Alkaline Phosphatase

19.

If the enzyme A was to be placed in the pH of 6, the enzyme would function less or not at all. Why did the function of enzyme A change?

a)

The pH change caused a structure of the polypeptide change

b)

the pH change caused the DNA to fold

c)

the pH caused the mRNA to coil and

d)

the pH caused the cell to lyse

20.

What is the optimal conditions for enzyme B?

a)

Temp of 78 degrees and pH of 8

b)

Temp of 50 degrees and pH of 4

c)

Temp of 90 degrees and pH of 3

d)

Temp of 78 degrees and pH of 2

21.

The diagram represents one way an enzyme can be inhibited. Which statement explains the effect of an inhibitor on an enzyme?

a)

A substrate will be able to bond with the enzyme.

b)

The enzyme will likely be attacked by immune cells

c)

The enzyme will be unable to produce more enzymes.

d)

A substrate will be unable to attach to the enzyme.

22.

What is an enzyme?

a)

A protein that speeds up chemical reactions

b)

A protein that breaks apart molecules

c)

A protein needed for cell structure

d)

A neurological protein involved in cell signaling

23.
Which of the factors does NOT affect enzymatic activity?
a)
Temperature
b)
pH
c)
Concentration
d)
Color
24.
With what 3 letters do enzymes typically end?
a)
-ose
b)
-ase
c)
-ese
d)
-tic
25.
What is the purpose  of an enzyme?
a)
give permission for the reaction
b)
raise activation energy
c)
speed up reactions
d)
control how many reactions occur
26.

All the genes in both a prokaryotic cell and a eukaryotic cell are expressed at the same time.

a)

True

b)

False

27.
What is the name given to part of a DNA molecule that codes for a protein?
a)
Glucose
b)
Chromosome
c)
Gene
d)
Phosphate
28.
Where does transcription occur in eukaryotic cells?
a)
Nucleus
b)
Ribosome
c)
Cytoplasm
d)
Rough E.R.
29.
Identify the SUBSTITUTION mutation from the following DNA sequence: 
ATG CCA AAT
a)
ATG TCA AAT
b)
ATG CCT AAA T
c)
ATC CA  AT
d)
ATG CCA AAT
30.
How can a person's muscle cells have the same exact DNA sequences as their nerve cells even though the look and perform completely different?
a)
The two different cells become mutated
b)
The proteins expressed in each cell are different
c)
They actually have different DNA in the two types of cells.
d)
The genome of the different cells changes
31.

What happens to an enzyme if the temperature decreases too low?

a)

No effect

b)

Enzyme increases rate

c)

Enzyme decreases rate

d)

Enzyme denatures

32.

When an enzyme becomes denatured, its active site loses it shape. This means that the ______________ chains in the protein (enzyme) become disrupted and cause the protein to unravel and lose its shape.

a)

hyrocarbon

b)

amino acid

c)

nucleic acid

d)

glycoprotein

33.

James is conducting an experiment on an enzyme commonly found in the human stomach. At a pH of 4, what could happen to this enzyme?

a)

It would catalyze the digestion of proteins at a faster rate.

b)

It would have no effect on its activity.

c)

It would function at its optimum activity level.

d)

It would possibly denature and lose function.

34.

What happens to an enzyme when it denatures?

a)

The activation energy of the reaction is doubled.

b)

The activation energy of the reaction is lowered.

c)

It's optimal conditions for temperature of the enzyme are doubled.

d)

The shape of the enzyme molecule is changed.

35.
Which of the following conclusions can be drawn from this graph?
a)
The optimum pH of the enzyme is 6.6.
b)
The optimum pH of the enzyme is 5.8
c)
The enzyme’s activity increases as pH increases 5.0 to 9.0
d)
The enzyme’s activity is greater around pH of 8.0 .
36.
Which of these is most likely an enzyme?  
a)
Sucrose
b)
Galactose
c)
Fructose
d)
Amylase
37.
What happens when enzymes are heated to a high temperature?
a)
The enzymes die.
b)
The shapes of the enzyme are altered denature
c)
The enzymes' amino acid sequence changes.
d)
The enzymes remain the same
38.
Letter C...
a)
active site
b)
enzymes
c)
substrate
d)
products
39.
Letter B...
a)
active site
b)
enzyme
c)
substrate
d)
products
40.
Letter D...
a)
active site
b)
enzyme
c)
substrate
d)
products
41.
Letter E...
a)
active site
b)
enzyme
c)
substrate
d)
products
42.

Which 2 factors can affect the rate of enzyme activity?

a)

Light intensity

b)

Temperature

c)

pH

d)

Oxygen concentration

43.
When a piece of liver is dropped into hydrogen peroxide, the peroxide bubbles vigorously as the hydrogen peroxide (H2O2) decomposes into water (H2O) and oxygen (O2). This rapid change in reaction rate suggests 
a)
that liver contains lots of germs
b)
 the liver contains an enzyme that breaks down hydrogen peroxide
c)
 that liver is a substrate for hydrogen peroxide
d)
that hydrogen peroxide is a powerful enzyme
44.
Which of these is NOT true?
a)

Enzymes can denature (change shape) when the temperature gets too high.

b)

Enzymes can only be used once in a chemical reaction. 

c)

Extreme pH can denature enzymes.

d)

Enzymes speed up chemical reactions. 

45.
Based on the graph, what is the optimal temperature for this enzyme? 
a)
15oC
b)
40oC
c)
30oC
d)
35oC