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WorksheetsAUBF MIDTERM EXAM
Total questions: 100
Worksheet time: 1hrs 12mins
Which of the following dyes are used in Sternheimer–Malbin stain?
Hematoxylin and eosin
Crystal violet and safranin
Methylene blue and eosin
Methylene blue and safranin
Which of the following statements regarding WBCs in urinary sediment is true?
"Glitter cells” seen in the urinary sediment are a sign of renal disease
Bacteriuria in the absence of WBCs indicates lower urinary tract infection (UTI)
WBCs other than PMNs are not found in urinary sediment
WBC casts indicate that pyuria is of renal, rather than lower urinary, origin
Which description of sediment with Sternheimer–Malbin stain is correct?
Transitional epithelium: cytoplasm pale blue, nucleus dark blue
Renal epithelium: cytoplasm light blue, nucleus dark purple
Glitter cells: cytoplasm dark blue, nucleus dark purple
Squamous epithelium: cytoplasm pink, nucleus pale blue
SITUATION: A 5-mL urine specimen is submitted for routine urinalysis and analyzed immediately. The SG of the sample is 1.012 and the pH is 6.5. The dry reagent strip test for blood is a large positive (3+) and the microscopic examination shows 11–20 RBCs per HPF. The leukocyte esterase reaction is a small positive (1+), and the microscopic examination shows 0–5 WBCs per HPF. What is the most likely cause of these results?
Myoglobin is present in the sample
Free hemoglobin is present
Insufficient volume is causing microscopic results to be underestimated
Some WBCs have been misidentified as RBCs
Which of the following statements regarding epithelial cells in the urinary system is correct?
Caudate epithelial cells originate from the upper urethra
Transitional cells originate from the upper urethra, ureters, bladder, or renal pelvis
Cells from the proximal renal tubule are usually round in shape
Squamous epithelium line the vagina, urethra, and wall of the urinary bladder
Which of the statements regarding examination of unstained sediment is true?
Renal cells can be differentiated reliably from WBCs
Large numbers of transitional cells are often seen after catheterization
Neoplastic cells from the bladder are not found in urinary sediment
RBCs are easily differentiated from nonbudding yeast
Which of the following statements regarding cells found in urinary sediment is true?
Transitional cells resist swelling in hypotonic urine
Renal tubular cells are often polyhedral and have an eccentric round nucleus
Trichomonads have an oval shape with a prominent nucleus and a single anterior flagellum
Clumps of bacteria are frequently mistaken for blood casts
Which of the following statements regarding RBCs in the urinary sediment is true?
Yeast cells will lyse in dilute acetic acid but RBCs will not
RBCs are often swollen in hypertonic urine
RBCs of glomerular origin often appear dysmorphic
Yeast cells will tumble when the cover glass is touched but RBCs will not
Renal tubular epithelial cells are shed into the urine in largest numbers in which condition?
Malignant renal disease
Acute glomerulonephritis
Nephrotic syndrome
Cytomegalovirus (CMV) infection of the kidney
The ova of which parasite may be found in the urinary sediment?
T. vaginalis
Entamoeba histolytica
Schistosoma hematobium
Trichuris trichiura
Oval fat bodies are often seen in
Chronic glomerulonephritis
Nephrotic syndrome
Acute tubular nephrosis
Renal failure of any cause
All of the following statements regarding urinary casts are true except:
Many hyaline casts may appear in sediment after jogging or exercise
An occasional granular cast may be seen in a normal sediment
Casts can be seen in significant numbers even when protein tests are negative
Hyaline casts will dissolve readily in alkaline urine
Which condition promotes the formation of casts in the urine?
Chronic production of alkaline urine
Polyuria
Reduced filtrate formation
Low urine SG
The mucoprotein that forms the matrix of a hyaline cast is called:
Bence–Jones protein
β-Microglobulin
Tamm–Horsfall protein
Arginine-rich glycoprotein
“Pseudocasts” are often caused by:
A dirty cover glass or slide
Bacterial contamination
Amorphous urates
Mucus in the urine
Which of the following statements regarding urinary casts is correct?
Fine granular casts are more significant than coarse granular casts
Cylindruria is always clinically significant
The appearance of cylindroids signals the onset of end-stage renal disease
Broad casts are associated with severe renal tubular obstruction
A sediment with moderate hematuria and RBC casts most likely results from:
Chronic pyelonephritis
Nephrotic syndrome
Acute glomerulonephritis
Lower urinary tract obstruction
Urine sediment characterized by pyuria with bacterial and WBC casts indicates:
Nephrotic syndrome
Pyelonephritis
Polycystic kidney disease
Cystitis
Which type of casts signals the presence of chronic renal failure?
Blood casts
Fine granular casts
Waxy casts
Fatty casts
When examining urinary sediment, which of the following is considered an abnormal finding?
0–2 RBCs per HPF
0–1 hyaline casts per low-power field (LPF)
0–1 renal cell casts per LPF
2–5 WBCs per HPF
SITUATION: A urine sample with a pH of 6.0 produces an abundance of pink sediment after centrifugation that appears as densely packed yellow- to reddish-brown granules under the microscope. The crystals are so dense that no other formed elements can be evaluated. What is the best course of action?
Request a new urine specimen
Suspend the sediment in prewarmed saline, then repeat centrifugation
Acidify a 12-mL aliquot with three drops of glacial acetic acid and heat to 56°C for 5 minutes before centrifuging
Add five drops of 1N HCl to the sediment and
How can hexagonal uric acid crystals be distinguished from cystine crystals?
Cystine is insoluble in hydrochloric acid but uric acid is soluble
Cystine gives a positive nitroprusside test after reduction with sodium cyanide
Cystine crystals are more highly pigmented
Cystine crystals form at neutral or alkaline pH, uric acid forms at neutral to acidic pH
The presence of tyrosine and leucine crystals together in a urine sediment usually indicates:
Renal failure
Chronic liver disease
Hemolytic anemia
Hartnup disease
Which of the following crystals is considered nonpathological?
Hemosiderin
Bilirubin
Ammonium biurate
Cholesterol
At which pH are ammonium biurate crystals usually found in urine?
Acid urine only
Acid or neutral urine
Neutral or alkaline urine
Alkaline urine only
Which of the following crystals is seen commonly in alkaline and neutral urine?
Calcium oxalate
Uric acid
Magnesium ammonium phosphate
Cholesterol
Which crystal appears in urine as a long, thin hexagonal plate, and is linked to ingestion of large amounts of benzoic acid?
Cystine
Hippuric acid
Oxalic acid
Uric acid
Small yellow needles are seen in the sediment of a urine sample with a pH of 6.0. Which of the following crystals can be ruled out
Sulfa crystals
Bilirubin crystals
Uric acid crystals
Cholesterol crystals
Oval fat bodies are derived from:
Renal tubular epithelium
Transitional epithelium
Degenerated WBCs
Mucoprotein matrix
Oval fat bodies are often associated with:
Lipoid nephrosis
Acute glomerulonephritis
Aminoaciduria
Pyelonephritis
Urine of constant SG ranging from 1.008 to 1.010 most likely indicates:
Addison’s disease
Renal tubular failure
Prerenal failure
Diabetes insipidus
Which of the following characterizes prerenal failure, and helps to differentiate it from acute renal failure caused by renal disease?
BUN:creatinine ratio of 20:1 or higher
Urine:plasma osmolal ratio less than 2:1
Excess loss of sodium in the urine
Dehydration
Which of the following conditions characterizes chronic glomerulonephritis and helps to differentiate it from acute glomerulonephritis?
Hematuria
Polyuria
Hypertension
Azotemia
Which of the following conditions is seen in acute renal failure and helps to differentiate it from prerenal failure?
Hyperkalemia and uremia
Oliguria and edema
Low creatinine clearance
Abnormal urinary sediment
Which of the following conditions characterizes acute renal failure and helps to differentiate it from chronic renal failure?
Hyperkalemia
Hematuria
Cylindruria
Proteinuria
The serum concentration of which analyte is likely to be decreased in untreated cases of acute renal failure?
Hydrogen ions
Inorganic phosphorus
Calcium
Uric acid
Which of the following conditions is associated with the greatest proteinuria?
Acute glomerulonephritis
Chronic glomerulonephritis
Nephrotic syndrome
Acute pyelonephritis
Which of the following conditions is often a cause of glomerulonephritis?
Hypertension
Cytomegalovirus infection
Systemic lupus erythematosus
Heavy metal poisoning
Acute pyelonephritis is commonly caused by:
Bacterial infection of medullary interstitium
Circulatory failure
Renal calculi
Antigen–antibody reactions within the glomeruli
All of the following are common characteristics of the nephrotic syndrome except:
Hyperlipidemia
Hypoalbuminemia
Hematuria and pyuria
Severe edema
Which of the following conditions is a characteristic finding in patients with obstructive renal disease?
Polyuria
Azotemia
Dehydration
Alkalosis
Whewellite and weddellite kidney stones are composed of:
Magnesium ammonium phosphate
Calcium oxalate
Calcium phosphate
Calcium carbonate
Which of the following abnormal crystals is often associated with formation of renal calculi?
Cystine
Ampicillin
Tyrosine
Leucine
Which statement about renal calculi is true?
Calcium oxalate and calcium phosphate account for about three-fourths of all stones
Uric acid stones can be seen by x-ray
Triple phosphate stones are found principally in the ureters
Stones are usually comprised of single salts
Alkaptonuria, a rare hereditary disease, is characterized by the urinary excretion of
Alkaptone
Phenylalanine
5-Hydroxyindole acetic acid
Homogentisic acid
A 22-year-old female clinical laboratory student performs a urinalysis on her own urine as part of a lab class. Significant results include: color = yellow; appearance = cloudy; pH = 7.5; nitrite = positive; leukocyte esterase - 2+; 25^0 WBC/hpf; 0-3 RBC/hpf; 2-5 squamous epithelial cells/hpf; moderate bacteria. All other chemistries and microscopic results were normal. These findings suggest
Glomerulonephritis
Upper urinary tract infection
Lower urinary tract infection
Nephrolithiasis
Metastatic carcinoid tumors arising from the enterochromaffin cells of the gastrointestinal tract are characterized by increased excretion of urinary
Serotonin
5-Hydroxytryptophan
Homogentisic acid
5-Hydroxyindole acetic acid
Some clinical conditions are characterized by unique urinalysis result patterns. Which of the following shows such a relationship?
Nephrotic syndrome: positive protein on reagent strip, negative protein with sulfosalicylic acid
Intensive dieting: increased ketones, negative glucose
Multiple myeloma: positive protein by both reagent strip and sulfosalicylic acid
Cystitis: positive nitrite and protein
Nitrite in a urine specimen suggests the presence of
White blood cells
Red blood cells
Bacteria
Yeasts
Phenylketonuria may be characterized by which of the following statements?
It may cause brain damage if untreated
It is caused by the absence of the enzyme, phenylalanine oxidase.
Phenylpyruvic acid excess appears in the blood
Excess tyrosine accumulates in the blood
Which of the following systems utilizes polyelectrolytes to determine the specific gravity of urine?
Refractometer
Osmometer
TS meter
Reagent strip
Which urinalysis reagent strip test will never be reported out as "negative"?
Protein
Urobilinogen
Bilirubin
Nitrite
Which of the following is a true statement?
Renal tubular cells originate from the renal pelvis.
Red blood cells in acid urine (pH 4.5) will usually be crenated because of the acidity
Bacteria introduced into a urine specimen at the time of the collection will have no immediate effect on the level of nitrite in the specimen
Pilocarpine iontophoresis is the method of choice for the collection of pericardial fluid.
Which of the following is a routine analysis test for the presence of ketone bodies in urine?
Rothera's test
Gmelin's test
Sulphosalicylic acid test
Hay's test
Turbidity in the urine sample indicates
pus
blood
bacteria
all of the above
Eosinophil count: >1%
Acute Interstitial Nephritis
UTI
Renal Transplant Rejection
Normal
Glitter cell
NEUTROPHIL
EOSINOPHIL
LYMPHOCYTE
SWOLLEN MONOCYTE
Seen in ethylene glycol poisoning
Dihydrate CaOx
Monohydrate CaOx
Both
Neither
Crystals seen in liver failure except?
Bilirubin
Leucin
Tyrosine
Ampicillin
Fatty cast can be seen in
Nephrotic syndrome
Toxic tubular necrosis
DM
All of the choices
None of the choices
Seen in Acute Interstitial Nephritis
RBC
Eosinophils
Bacteria
RBC and Bacteria
RBC and Eosinophils
The presence of this compound may indicate liver disease
bilirubin
hemoglobin
ammonia
intact RBCs
Which of the following is the pigment that gives urine its characteristic color
hemoglobin
biliverdin
homogentisic acid
urochrome
Hyposthenuria is urine with
consistently low specific gravity
consistently high specific gravity
normal specific gravity
specific gravity of 1.000
Which of the following is the best way to perform the chemical exam
dipping the dipstick, then standing it upright and reading
submerging the dipstick in the urine for 1 minute then reading
quickly submerging the dipstick and removing excess urine, then placing horizontally to read
all answers are acceptable
This is a test to screen for UTIs
bilirubin
ketones
leukocyte esterase
pH
Which of the following tests screen for diabetes mellitus?
bilirubin/urobilinogen
ketones/glucose
nitrite/leukocyte esterase
blood/specific gravity
In reporting SSA turbidity, distinct turbidity is seen with no granulation:
1+
2+
3+
4+
In reporting SSA turbidity, turbidity is seen with granulation but without flocculation
1+
2+
3+
4+
The parameter in the reagent strip which utilizes Ehrlich units is:
Bilirubin
Urobilinogen
Glucose
Leukocytes
Ascorbic acid causes false negative reactions in the following urine reagent strip except?
Blood
Bilirubin
Leukocytes
pH
Ascending sequence of casts:
Waxy-hyaline-coarsely granular-finely granular-cellular
Coarsely granular-finely granular-hyaline-waxy-cellular
Hyaline-cellular-coarsely granular-finely granular-waxy
Cellular-hyaline-finely granular-coarsely granular-waxy
Normal urinary crystal which is colorless, resembling flat plates or thin prisms often in rosette form:
Amorphous phosphates
Struvite
Apatite
Calcium carbonate
Manner of reporting for RTE cells:
Average number per LPF
Average number per HPF
Average number per OIO
1+, 2+, 3+, 4+
Transitional epithelial cells seen in urine specimens may be reported using rare/few/moderate/many by using the
Scanner field
Low power field
High power field
Oil immersion field
A renal calculi described as very hard, dark in color with rough surface:
Calcium oxalate
Uric acid
Cystine
Phosphate
Renal disease whose etiology is the deposition of anti-glomerular basement membrane antibody to glomerular and alveolar basement membranes:
Berger’s disease
Wegener’s granulomatosis
Goodpasture syndrome
Membranous glomerulonephritis
The purpose of the Hansel stain is to identify:
Neutrophils
Monocytes
Renal tubular cells
Eosinophils
Blue diaper syndrome is associated with
Lesch-Nyhan syndrome
Hartnup disease
Alkaptonuria
Dubin-Johnson syndrome
Which of the following statements regarding WBCs in urinary sediment is true?
“Glitter cells” seen in the urinary sediment are a sign of renal disease
Bacteriuria in the absence of WBCs indicates lower urinary tract infection (UTI)
WBCs other than PMNs are not found in urinary sediment
WBC casts indicate that pyuria is of renal, rather than lower urinary, origin
Which type of casts signals the presence of chronic renal failure?
Blood casts
Fine granular casts
Waxy casts
Fatty casts
Whewellite and weddellite kidney stones are composed of
Magnesium ammonium phosphate
Calcium oxalate
Calcium phosphate
Calcium carbonate
A strong odor of cabbage in a urine specimen could indicate:
Methionine malabsorption
Trimethylaminuria
Phenylketonuria
Tyrosyluria
Which of these plasma substances is NOT normally filtered through the glomerulus in significant amounts?
Creatinine
Glucose
Urea
Protein
Which of the following can give a false negative urine protein reading?
contamination with vaginal discharge
heavy mucus
presence of blood
very dilute urine
Upon standing at room temperature, a urine pH typically
decreases
increases
remains the same
changes depending on the bacterial concentration
Polarized light can be often be used to differentiate between
fibers and mucus clumps
hyaline and waxy casts
squamous and transitional epithelial cells
red blood cells and white blood cells
Which of the following contaminant has a dimpled center and will polarize?
starch
oil droplets
air bubbles
pollen grains
Bacteria re considered significant in the urine sediment when the
nitrate is positive
protein is positive
specimen is cloudy
leukocyte is positive
Which of the following positive chemical reactions is most closely associated with the presence of yeast in the urine sediment?
nitrite
protein
glucose
blood
A 21 year old woman had glucose in her urine with normal blood sugar. These findings are most consistent with:
renal glycosuria
diabetes mellitus
diabetes insipidus
alkaline tide
Following ingestion of ethylene glycol(antifreeze) numerous crystals are found in the urine. The shape of these crystals is:
flat with notched corners
oval/dumbbell
coffin-lid
rosettes/rhomboid
After warming, a cloudy urine clears, This is due to the presence of:
urates
phosphates
WBCs
bacteria
Which of the following crystals may be found in acidic urine?
calcium carbonate
calcium oxalate
calcium phosphate
triple phosphate
Using polarized light microscopy, which of the following urinary elements are birefringent?
cholesterol
triglycerides
fatty acids
neutral acids
The possibility of detecting glitter cells is associated with urine that is
acidic
dilute
alkaline
concentrated
Hyaline casts are usually found in:
in the center of the coverslip
under subdued light
under very bright light
in the supernatant
A 2 year old child had positive urine ketone. This would most likely be caused by:
vomiting
anemia
hypoglycemia
biliary tract obstruction
Which of the following reagents is used to react with ketones in the urine?
sodium nitroprusside
acetoacetic acid
acetone
beta-hydroxybutyric acid
A reagent strip area impregnated with stabilized, diazotized 2, 4 dichloroaniline will yield a positive reaction with:
bilirubin
hemoglobin
ketones
urobilinogen
