Font size
WorksheetsScreening of Anaerobic MO_SSCG 3673 2024/25 UTM
Total questions: 12
Worksheet time: 3mins
Which of these DO NOT require oxygen to survive?
Microaerophilic and Aerotolerant MOs
Aerotolerant MOs and Obligate anaerobes
Facultative anaerobes and Obligate aerobes
Obligate anaerobes, Microaerophiles and Aerotolerant MOs
Point out the toxic forms of Oxygen.
H2O2
OH. and H2O
H2O
O2- and OH.
Recommend a suitable enzyme which catalyzes the conversion of O2- to H2O2.
SOD
Catalase
Peroxidase
Complete the table.
A: Obligate aerobe
B: Facultative anaerobe (in absence of oxygen)
C: Obligate anaerobe
A: SOD & Peroxidase
B: SOD & Catalase
C: All (3)
A: SOD & Catalase
B: SOD & Peroxidase
C: None of the (3)
A: SOD & Catalase
B: SOD & Peroxidase
C: SOD & Peroxidase
A: SOD & Peroxidase
B: SOD & Catalase
C: None of the (3)
Classify Clostridium botulinum.
facultative anaerobe
aerotolerant MO
Obligate anaerobe
Microaerophile
Highlight the MOST crucial requirement in isolating
C. tetani?
Reducing agent
Avoidance of O2
glucose
urea
Describe the figure.
isolate O2 sensitive MOs
use of gassing cannula
Rolling tube technique
anaerobic chamber
methylene blue strip
Based on these description, point out the technique/ method.
- suitable for isolating strict anaerobes
- isolated without an anaerobic chamber
- Colonies form throughout the depth of the agar column
-strict anaerobes grow at the bottom of the tube where the oxygen tension is low
Rolling Tube
Anaerobic Jar
Agar Shake
Hungate
The Gas Pak system includes........
gassing cannula
methylene blue strips
palladium
culture tubes
H2 & CO2 generator envelope
Which of these are related to MO culture maintenance?
glycerol or DMSO in medium
Long term storage of 6 months
freeze drying for obligate anaerobes
a final concentration of 10% v/v DMSO
Short term storage: Kept frozen (-20 to -80oC)
Biogas is a renewable natural gas.
True
False
Biogas is a product of anaerobic digestion of organic wastes. Point out facts on Biogas.
can be used as fuel gas
used in stationary engines to generate electricity
free from CO2
Consists of 70% CH4
Biomethane can be processed from biogas
