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Clinical Chemistry Midterm

Total questions: 71

Worksheet time: 30mins

Name
Class
Date
1.

The Largest and least dense of lipoprotein.

a)

HDL

b)

LDL

c)

VLDL

d)

Chylomicron

2.

Three fatty acids attached to One molecule of glycerol by ester bonds

a)

Fatty Acids

b)

Saturated Fat

c)

Unsaturated fat

d)

Triglycerides

3.

It is Produced by the liver and a major carrier of endogenous triglyceride

a)

VLDL

b)

HDL

c)

LDL

d)

Chylomicron

4.

Form as a result of lipolysis of VLDL, Readily taken up by cells via LDL receptors in Liver and peripheral cells, can infiltrate extracellular space

a)

IDL

b)

LDL

c)

HDL

d)

Chylomicron

5.

Also known as the alpha lipoprotein

a)

VLDL

b)

LDL

c)

HDL

d)

Chylomicron

6.

Also known as the beta Lipoprotein

a)

VLDL

b)

LDL

c)

HDL

d)

Chylomicron

7.

Also known as the Pre-beta Lipoprotein

a)

VLDL

b)

LDL

c)

HDL

d)

Chylomicron

8.

It is similar to triglycerides, except with only two esterified fatty acids

a)

Cholesterol

b)

Triglycerides

c)

Phospholipids

d)

Protein

9.

An unsaturated steroid alcohol containing Four rings and Single side chain tail

a)

Cholesterol

b)

Protein

c)

Fat

d)

Triglyceride

10.

Smallest and most dense. It synthesized by the Liver and Intestine .It is capable of removing excess cholesterol from peripheral cells

a)

VLDL

b)

LDL

c)

HDL

d)

Chylomicron

11.

Abnormal lipoprotein, patients with biliary cirrhosis or Cholestasis, LCAT mutations

a)

Lipoprotien X

b)

Lipoprotein A-10

c)

Lipoprotein B-24

d)

Lipoprotein B-200

12.

Single leading cause of death & disability in the US Caused by lipids, in form of esterified cholesterol being deposited in artery walls resulting in fatty streaks

a)

Arteriosclerosis

b)

Atherosclerosis

c)

Hyperlipoproteinemia

d)

Diabetes

13.

Lipids are basically ?

a)

Enzymes

b)

Protein

c)

Fats

d)

Phospholipids

14.

It is a major class of dietary lipids which comprises of 95% of lipids in food and human body.

a)

Phospholipids

b)

Fats

c)

Triglycerides

d)

Cholesterol

15.

It is the precursor of steroid hormones and it is not used for energy.

a)

Phospholipid

b)

Fats

c)

Triglyceride

d)

Cholesterol

16.

Select the correct answer. What lipoprotein is the most dense.

a)

VLDL

b)

LDL

c)

HDL

d)

Chylomicrons

17.

Select the correct answer. What lipoprotein is the least dense.

a)

VLDL

b)

LDL

c)

HDL

d)

Chylomicrons

18.

Select the correct answer. What lipoprotein is the least dense.

a)

VLDL

b)

LDL

c)

HDL

d)

Chylomicrons

19.

Select the correct answer. What lipoprotein is the heaviest?

a)

VLDL

b)

LDL

c)

HDL

d)

Chylomicrons

20.

Select the correct answer. What lipoprotein is the lightest?

a)

VLDL

b)

LDL

c)

HDL

d)

Chylomicrons

21.

What lipoprotein is responsible for post-prandial turbidity of sample?

a)

VLDL

b)

LDL

c)

HDL

d)

Chylomicron

22.

What lipoprotein is responsible for fasting hyperlipidemic turbidity of the sample?

a)

VLDL

b)

LDL

c)

HDL

d)

Chylomicron

23.

What lipoprotein is known as the bad cholesterol?

a)

VLDL

b)

LDL

c)

HDL

d)

Chylomicron

24.

The lipoprotein that is involved in the progression of Cardiovascular disease like atherosclerosis or stroke.

a)

VLDL

b)

LDL

c)

HDL

d)

Chylomicron

25.

What lipoprotein reveres cholesterol transport? It transport excess cholesterol from tissues of cells back to the liver.

a)

VLDL

b)

LDL

c)

HDL

d)

Chylomicron

26.

A type of protein that binds lipids to form lipoprotein. They serve as enzyme cofactors and receptor ligands and they also regulate the metabolism of Lipoprotein and their uptake in tissues.

a)

High Density Lipoprotein

b)

Low Density Lipoprotein

c)

Very Low Density Lipoprotein

d)

Chylomicrons

e)

Apolipoproteins

27.

An apolipoprotein that activates Chylomicron.

a)

Apo B-48

b)

Apo B-100

c)

Apo A-1

d)

Apo C-II

e)

Apo E

28.

Lipoprotein carried/function for VLDL and LDL.

a)

Apo B-48

b)

Apo B-100

c)

Apo A-1

d)

Apo C-II

e)

Apo E

29.

Lipemia Clearing factor that activates LPL, Targets the TAG and removes CM after meal.

a)

Apo B-48

b)

Apo B-100

c)

Apo A-1

d)

Apo C-II

e)

Apo E

30.

It is the main protein in HDL; and an activator of LCAT for Esterification.

a)

Apo B-48

b)

Apo B-100

c)

Apo A-1

d)

Apo C-II

e)

Apo E

31.

This Apolipoprotein is increased in Alzheimer's disease.

a)

Apo B-48

b)

Apo B-100

c)

Apo A-1

d)

Apo C-II

e)

Apo E

32.

An accumulation of sphingomyelin in the bone marrow, spleen and lymph nodes

a)

Niemann-Pick Disease

b)

Tangier's Disease

c)

Tay-Sach's Disease

d)

Anderson's Disease

e)

Sitosterolemia

33.

It is the complete absence of HDL (HDL: 1-2 mg/dL)

Clinical characteristic: orange or yellow discoloration of tonsils and pharynx

a)

Niemann-Pick Disease

b)

Tangier's Disease

c)

Tay-Sach's Disease

d)

Anderson's Disease

e)

Sitosterolemia

34.

Deficiency of hexosaminidase A and an accumulation of sphingolipids in the brain

a)

Niemann-Pick Disease

b)

Tangier's Disease

c)

Tay-Sach's Disease

d)

Anderson's Disease

e)

Sitosterolemia

35.

It is a Chylomicron-retention disease

a)

Niemann-Pick Disease

b)

Tangier's Disease

c)

Tay-Sach's Disease

d)

Anderson's Disease

e)

Sitosterolemia

36.

Plant sterols absorbed and accumulated in the blood and tissues.

a)

Niemann-Pick Disease

b)

Tangier's Disease

c)

Tay-Sach's Disease

d)

Anderson's Disease

e)

Sitosterolemia

37.

Specimen Considerations for Lipids. It requires at least how many hours of fasting?

a)

8-10 hours of fasting

b)

10-12 hours of fasting

c)

10-16 hours of fasting

d)

12-24 hours of fasting

38.

What sample is not affected by fasting?

a)

Fats

b)

Triglycerides

c)

Cholesterol

d)

Phospholipids

39.

Specimen used is?

a)

Serum only

b)

Plasma only

c)

Heparinized Plasma

d)

Serum or plasma EDTA

40.

What are the 3 step method for cholesterol measurement?

a)

1. Saponification

2. Colorimetry

3. Extraction

b)

1. Saponification

2. Extraction

3. Colorimetry

c)

1. Extraction

2. Saponification

3. Colorimetry

d)

1. Colorimetry

2. Saponification

3. Extraction

41.

What is the end product of Abell-kendall method?

a)

acetic anhydride

b)

cholestadienyl monosulfuric acid

c)

cholestadienyl disulfuric acid

d)

concentrated H2SO4

42.

What is the end product of Salkowski method?

a)

acetic anhydride

b)

cholestadienyl monosulfuric acid

c)

cholestadienyl disulfuric acid

d)

concentrated H2SO4

43.

What are the end color for both Abell-kendall method and the Abell,Levy,Brody method?

a)

Green & Yellow

b)

Blue-Green

c)

Just Yellow

d)

Just Green

44.

What cholesterol measurement uses the 3 step method?

a)

Abell-Kendall Method

b)

Abell, Levey, Brody Method

c)

Sperry Method

d)

Salkowski Method

45.

What cholesterol measurement uses the 4 step method?

a)

Abell-Kendall Method

b)

Abell, Levey, Brody Method

c)

Sperry Method

d)

Salkowski Method

46.

What are the 4-step method?

a)

1. Saponification

2. Extraction

3. Colorimetry

4. Purification

b)

1. Saponification

2. Extraction

3. Purification

4. Colorimetry

c)

1. Purification

2. Saponification

3. Extraction

4. Colorimetry

d)

1. Purification

2. Extraction

3. Saponification

4. Colorimetry

47.

What is the old reference method for cholesterol measurement?

a)

Salkowski Method

b)

Abell-Kendall Method

c)

Enzymatic Method

d)

Isotope Dilution/Mass Spectrometry

e)

Abell, Levey, Brody Method

48.

What is the current reference method for cholesterol measurement?

a)

Salkowski Method

b)

Abell-Kendall Method

c)

Enzymatic Method

d)

Isotope Dilution/Mass Spectrometry

e)

Abell, Levey, Brody Method

49.

What is the most commonly used method for cholesterol measurement?

a)

Salkowski Method

b)

Abell-Kendall Method

c)

Enzymatic Method

d)

Isotope Dilution/Mass Spectrometry

e)

Abell, Levey, Brody Method

50.

What is the definitive method for cholesterol measurement?

a)

Salkowski Method

b)

Abell-Kendall Method

c)

Enzymatic Method

d)

Isotope Dilution/Mass Spectrometry

e)

Abell, Levey, Brody Method

51.

What is cholesterol measurement method has an end color of Red?

a)

Salkowski Method

b)

Abell-Kendall Method

c)

Enzymatic Method

d)

Isotope Dilution/Mass Spectrometry

e)

Abell, Levey, Brody Method

52.

A triglyceride measurement method that uses a chromotropic acid reagent.

a)

Van Handel & Zilversmit Method

b)

Modified Van Handel & Zilversmit Method

c)

Hantzsch Method

53.

A triglyceride measurement method that uses a sulfuric acid or salicylic acid reagent.

a)

Van Handel & Zilversmit Method

b)

Modified Van Handel & Zilversmit Method

c)

Hantzsch Method

54.

A triglyceride measurement method that is a colorimetric method.

a)

Van Handel & Zilversmit Method

b)

Modified Van Handel & Zilversmit Method

c)

Hantzsch Method

55.

A triglyceride measurement method that is a colorimetric method and is a reference method.

a)

Van Handel & Zilversmit Method

b)

Modified Van Handel & Zilversmit Method

c)

Hantzsch Method

56.

A triglyceride measurement method that is a fluorometric method.

a)

Van Handel & Zilversmit Method

b)

Modified Van Handel & Zilversmit Method

c)

Hantzsch Method

57.

The end color for Van Handel & Zilversmit Method is?

a)

Blue

b)

Green

c)

Yellow

d)

Pink

58.

The end color for Modified Van Handel & Zilversmit Method is?

a)

Blue

b)

Green

c)

Yellow

d)

Pink

59.

The end color for Hantzsch Method is?

a)

Blue

b)

Green

c)

Yellow

d)

Pink

60.

What are the floating layers in lipoprotein analysis?

a)

Chylomicron and LDL

b)

HDL and VLDL

c)

HDL and LDL

d)

Chylomicron and VLDL

61.

What are the sinking layers of Lipoprotein analysis?

a)

Chylomicron and VLDL

b)

Chylomicron and HDL

c)

VLDL and LDL

d)

HDL and LDL

e)

HDL and VLDL

62.

What is the preferred gel in the electrophoresis of lipoproteins?

a)

Agarose Gel

b)

Cellulose acetate

c)

Polyacrylamide

d)

Nitrocellulose

63.

The word proteins come from the Greek word "proteis" which means?

a)

Energy providing substance

b)

Fuel for the engine

c)

Important building block for life

d)

First rank of importnce

64.

Proteins are amphoteric. What dos Amphoteric mean?

a)

It is not able to react both as a base and as an acid

b)

It is able to react both as a base and as an acid

c)

It can act as a base only

d)

It can act as an acid only

65.

All of the following are ethe biological function of proteins except one.

a)

Contraction and relaxation of muscles, also provides maintenance of osmotic pressure

b)

Structural support for the tissue (collagen and keratin)

c)

Coagulation and immunologic function

d)

Precursor of hormones and transports metabolic substances

e)

Acts as a biochemical inhibitor

66.

It is a linear sequence of amino acids and it determines the identity of protein, molecular structure, function and binding capacity. It is bound together by peptide bond.

a)

Primary Structure

b)

Secondary Structure

c)

Tertiary Structure

d)

Quaternary Structure

67.

It refers to specific regular three-dimensional formations into which portions of the polypeptide chain fold

a)

Primary Structure

b)

Secondary Structure

c)

Tertiary Structure

d)

Quaternary Structure

68.

An actual 3-dimentional structure or folding pattern and is responsible for physical and chemical properties of proteins

a)

Primary Structure

b)

Secondary Structure

c)

Tertiary Structure

d)

Quaternary Structure

69.

The association of 2 or more polypeptide chains

a)

Primary Structure

b)

Secondary Structure

c)

Tertiary Structure

d)

Quaternary Structure

70.

A type of protein that contains peptide chains which on hydrolysis will yield only amino acids.

a)

Simple Proteins

b)

Conjugated Proteins

c)

Complicated Proteins (mixed)

d)

Unconjugated Proteins

71.

It contains protein and non-protein group

a)

Simple Proteins

b)

Conjugated Proteins

c)

Complicated Proteins (mixed)

d)

Unconjugated Proteins

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