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WorksheetsRespiration and Lipid Metabolism; Stress Physiology (2/2)
Total questions: 66
Worksheet time: 40mins
1
(a)
2
(a)
3
(a)
4
(a)
Change in morphology like makahiya thorns
Avoidance strategy
Tolerance strategy
Chemical repellants
Avoidance strategy
Tolerance strategy
Dormancy during harsh winter
Avoidance strategy
Tolerance strategy
Hormonal mediation
Defensive strategy
Tolerance strategy
And/or
In the case of exposure to a unidirectional moving fluid, which is/are the plant avoidance strategy/ies
minimization of the drag coefficient (streamlined form)
minimization of the frontal area (small growth form)
maximization of strength (more strengthening tissues)
maximization of cross-sectional area (increased radial growth)
In the case of exposure to a unidirectional moving fluid, which is/are the plant tolerance strategy/ies
minimization of the drag coefficient (streamlined form)
minimization of the frontal area (small growth form)
maximization of strength (more strengthening tissues)
maximization of cross-sectional area (increased radial growth)
Plant recovery from stress depends on:
Stress severity
Acclimation
Adaptation
Stress frequency
Introduction of milder stress before extreme stress
Stress severity
Acclimation
Adaptation
Changes in genes occurring through time to withstand stress
Stress severity
Acclimation
Adaptation
Solid lines indicate:
survival without acclimation
survival with acclimation
genotypes of one species differing in acclimation potential
related species with greater tolerance
Dashed lines indicate:
survival without acclimation
survival with acclimation
genotypes of one species differing in acclimation potential
related species with greater tolerance
Blue lines indicate:
survival without acclimation
survival with acclimation
genotypes of one species differing in acclimation potential
related species with greater tolerance
Red lines indicate:
survival without acclimation
survival with acclimation
genotypes of one species differing in acclimation potential
related species with greater tolerance
How did Latus tenuis recover from submergence/water flooding
Increased shoot length compared to control
Increased root length compared to control
Decreased shoot porosity compared to control
Decreased root porosity compared to control
What is this recovery from (1-2 words, either term is ok)
(a)
Stressed or not?
Yes
No
Miss na kita, balik ka na </3
Jason Derulo
Stressed or not?
Yes
No
Miss na kita, balik ka na </3
Jason Derulo
BCA, infected soil
Green
White
Blue
Depending on species and environmental conditions, respiration consumes ___% of all the carbohydrates produced in photosynthesis - even more at extremely slow growth rates.
25-75
30-80
20-70
30-70
Respiration in plants can also proceed in a manner that produces heat. This type of respiration involves the cyanide-resistant, alternative _______; it is unique to plants, and resides in the mitochondria.
oxidase
reductase
Plant respiration: Glucose is most commonly cited as the substrate for respiration. However, in a functioning plant cell the reduced carbon is derived from sources such as the disaccharide sucrose, hexose phosphates, and triose phosphates from starch degradation and photosynthesis, fructose-containing polymers (fructans), and other sugars, as well as lipids (primarily triacylglycerols), organic acids, and on occasion proteins.
True
False
Typically, there are about ____ mitochondria per cell, representing around __% of the cell volume.
2000, 25
3000, 30
The inner mitochondrial membrane is completely ____________ even to small molecules (with the exception of O2, CO2, and H2O)
impermeable
permeable
Both mitochondrial membranes are rich in
proteins
porins
Mitochondria also helps to maintain proper concentration of calcium ions within the compartments of the cell
True
False
Charged molecules combine with oxygen and produce ATP molecules in a process called:
(a)
The plant cell mobilizes the large amount of free energy released in the oxidation of sucrose in a series of step-by-step reactions.
glycolysis
reactions of the pentose phosphate pathway
citric acid cycle
oxidative phosphorylation
Glycolysis: In a subsequent energy-conserving phase, the triose phosphates are oxidized and rearranged to yield two molecules of ________.
pyruvate
hexose phosphates
ATP
Besides preparing the substrate for oxidation in the citric acid cycle, (a) yields a small amount
of chemical energy
In most plant tissues, sucrose synthase is used to break down sucrose by combining it with uridine diphosphate glucose (UDP) to produce fructose and UDP-glucose. The UDP-glucose pyrophosphorylase enzyme then converts UDP-glucose and pyrophosphate (PPi) into uridine triphosphate (UTP) and glucose-6-phosphate.
Cytosolic glycolysis
Plastidic glycolysis
Converts starch into triose phosphates using a separate set of glycolytic isozymes that convert hexose phosphates to triose phosphates.
Cytosolic glycolysis
Plastidic glycolysis
The __________ glycolytic glyceraldehyde 3-phosphate dehydrogenase (GAPCp) converts glyceraldehyde-3-phosphate (GAP) into 1,3-bisphosphoglycerate (1,3-bisPGA), which is then converted into 3-phosphoglycerate (3-PGA) by __________ phosphoglycerate kinase (PGKp).
Cytosolic
Plastidial
Assimilation of CO2 during photosynthesis leads to 3-PGA production, which is then converted into triose phosphates by the sequential reactions of the photosynthetic isoforms of PGK and GAPDH (GAPA/GAPB). The 3-PGA production through glycolysis would then be a wasteful process in chloroplasts at least during the _____. Because of this, it was postulated that in chloroplasts, glycolysis would occur in the _____ when the Calvin cycle is not operative (Anoman et al., 2016).
day, dark
dark, day
The Initial phase of glycolysis. The substrates from different sources are channelled into triose phosphate. For each molecule of sucrose that is metabolized, _ molecules of triose phosphate (glyceraldehyde-3-phosphate) are formed. The process also requires an input of up to _______
4
3
5
An alternative end product, phosphoenolpyruvate, can be converted to malate for mitochondrial oxidation; NADH can be reoxidized during ____________ by either lactate dehydrogenase or alcohol dehydrogenase
fermentation
oxidative phosphorylation
Control of the oxidative pathway. The oxidative pentose phosphate pathway is controlled by the initial reaction of the pathway catalyzed by glucose-6-phosphate dehydrogenase, the activity of which is markedly inhibited by a high ratio of NADPH to NADP+.
True
False
The little operation of the oxidative pathway is likely to occur in the chloroplast because the end products of the pathway, fructose-6-phosphate and glyceraldehyde-3-phosphate, are being synthesized by the _______________. Therefore, the mass action will drive the non-oxidative interconversions of the pathway in the direction of pentose synthesis.
Calvin cycle
glycolysis
The vast majority of ATP is synthesized in the process called oxidative phosphorylation. Oxidative phosphorylation is linked to a process known as electron transport.
True
False
If electron transport stops or if the inner mitochondrial membrane’s impermeability to protons is compromised, oxidative phosphorylation will _____ occur
not
still
For each molecule of sucrose oxidized through glycolysis and the citric acid cycle pathways, 4 molecules of NADH are generated in the cytosol and 16 molecules of NADH plus 4 molecules of FADH2 are generated in the mitochondrial matrix.
True
False
The Electron Transport Chain catalyzes a flow of electrons from ____ to ____
NADH, O2
O2, NADH
The electron transport chain of plants contains the same set of electron carriers found in mitochondria from other organisms.
True
False
Electrons from NADH generated in the mitochondrial matrix during the citric acid cycle are oxidized by
complex I
complex II
complex III
complex IV
Is a membrane bound enzyme of the citric acid cycle that plays a role in the electron transport process, transferring electrons from its covalently bound FADH2 to coenzyme Q.
complex I
complex II
complex III
complex IV
The complex contains 11 subunits, a 2-iron ferredoxin, cytochromes b and c1 and belongs to the family of oxidoreductase enzymes. It accepts electrons from coenzyme Q in electron transport and passes them off to cytochrome c. In this cycle, known as the Q cycle, electrons arrive from CoQ in pairs, but get passed to cytochrome c individually.
complex I
complex II
complex III
complex IV
This complex contains two copper centers (CuA and CuB) and two cytochromes (see figure below, right-side); is the terminal oxidase and brings about the four-electron reduction of O2 to two molecules of H2O. Two protons are pumped per electron pair. Both structurally and functionally, ubiquinone and the cytochrome bc1 complex are very similar to plastoquinone and the cytochrome b6f complex, respectively, in the photosynthetic electron transport chain
complex I
complex II
complex III
complex IV
Unique Electron Transport Enzymes to Plant Mitochondria
Two NAD(P)H dehydrogenases, both Ca2+-dependent, attached to the outer surface of the inner membrane facing the intermembrane space that can oxidize cytosolic NADH and NADPH.
Rotenone-resistant dehydrogenase, NDin(NADH), for the oxidation of NADH derived from citric acid cycle substrates
An NADPH dehydrogenase, NDin(NADPH), is present on the matrix surface. Very little is known about this enzyme.
Alternative oxidase that is insensitive to inhibition by cyanide, azide, or carbon monoxide
The malonate, malate, and oxaloacetate inhibit the movement of electrons through:
complex I
complex II
complex III
complex IV
Common inhibitors of electron transport include rotenone and amytal, which stop movement of electrons past:
complex I
complex II
complex III
complex IV
The antimycin A, stops the movement of electrons that pass:
complex I
complex II
complex III
complex IV
Cyanide, carbon monoxide, azide, and hydrogen sulfide, inhibit electron movement in:
complex I
complex II
complex III
complex IV
It is the protein complex harvesting energy from the proton gradient and using it to make ATP from ADP.
(a)
Rotenone, amytal, antimycin inhibit:
ATP synthase
complex I
complex II
complex III
complex IV
The complete oxidation of a sucrose molecule leads to the net formation of: 8 ATP, 20 NADH, 4 FADH2
True
False
Since (a) cycles can yield heat, they are used as sources of heat in some types of tissue by the organisms
Factors affecting respiration
The alternative oxidase
The uncoupling protein
NADH dehydrogenase NDin(NADH)
ADP
Environmental factors
Which statement is false
Plants respire roughly half of the daily photosynthetic yield.
Respiration cannot operate during photosynthesis.
Different tissues and organs respire at different rates.
Mitochondrial function is crucial during pollen development
The biosynthesis of triacylglycerols and polar glycerolipids requires the cooperation of two organelles: the plastids and the:
ER
cytosol
In plants, fatty acids are synthesized exclusively in the
plastids
ER
mitochondria
In ____________, the prokaryotic pathway utilizes the 16:0- and 18:1-ACP products of chloroplast fatty acid synthesis to synthesize phosphatidic acid and its derivatives.
the cytoplasm
chloroplasts
In ____________, the eukaryotic pathway uses a separate set of acyltransferases in the ER to incorporate the fatty acids into phosphatidic acid and its derivatives.
the cytoplasm
chloroplasts
Which statement is false
Lipid composition influences membrane function.
Membrane lipids are precursors of important signaling compounds.
Storage lipids are converted into carbohydrates in senescing plants.
