WorksheetsMicrobiology
Total questions: 47
Worksheet time: 47mins
What atmospheric condition is needed to recover Campylobacter spp. from specimens inoculated onto a Campy-selective agar at 35-37oC and 42oC?
5% O2, 10% CO2, and 85% N2
20% O2, 20% CO2, and 60% N2
The bisphasic medium known as the Castaneda bottle is the preferred culture system for the cultivation of:
Brucella suis
Bordetella pertussis
H. ducreyi
In addition to CO2 requirement and biochemical characteristics. Brucella melitensis and Brucella abortus are differentiated by growth on media containing which two dyes?
basic fuchsin and thionine
carbol fuchsin and iodine
methylene blue and crystal violet
safranin and methylene blue
which medium is best for recovery of Legionella pneumophila from clinical specimens?
chocolate agar
new yeast extract agar
bordet-gengou agar
buffered caharcoal yeast extract agar
Which microorganism will grow only on a culture media that has been supplemented with either cysteine or cystine?
B. bacilliformis
K. kingae
F. tularensis
M. caterrhalis
Which of the following is the best rapid, noncultural test to perform when Gardnereila vaginalis is suspected in a patient with vaginosis?
10% H2O2
3% H2O2
30% H2O2
all of these
The appropriate culture media for the recovery of Gardnerella vaginalis is:
BCYE agar
HBT agar
CIN agar
Middlebrook agar
Which of the following species of Bacillus is nonmotile?
B. cereus
B. subtilis
B. anthracis
B. stearothermophilus
Bacillus anthracis and Bacillus cereus can best be differentiated by which tests?
motility and beta-hemolysis on blood agar plate
oxidase and alpha-hemolysis on blood agar plate
lecithinase and glucose
lecithinase and catalase
Spore-forming cells in bamboo pole arrangement of Bacillus anthracis can be found in:
specimen from patient
cultures
Which spore type and location is found on C, tetani?
round, terminal spores
ovoid, subterminal spores
round, subterminal
oval, terminal spores
To confirm a laboratory diagnosis of tetanus, it is essential to:
grow characteristic colonies through anaerobic cultures
demonstrate toxin production
find typical sporulating bacilli in smears
Which of the following is the MOST POTENT EXOTOXIN known?
botulinal toxin
erythrogenic toxin
tetanospasmin
alpha toxin
A positive lipase reaction is demonstrated by:
C. septicum
C. perfringens
C. botulinum
On tellurite medium, the colonies of C. diphtheriae are opaque, raised, convex and:
White
Green
Gun metal in color
Biotypes can be differentiated based on colonial morphology, biochemical reactions, and hemolytic patterns on blood agar. Which biotype of C. diphtheriae cause the most severe form of the disease?
Gravis
Intermedius
Beifanti
Mitis
Which two tests can be used to differentiate C. diphtheriae from other corynebacteria?
halo on tinsdale and nitrate
halo on tinsdale and urea
black on tinsdale and esculin
black on tinsdale and nitrate
Corynebacterium amycolatum is a NORMAL FLORA of the:
Human conjunctive
Skin
Nasopharynx
All of these
Growth in a 48-hour gelatin stab culture that shows lateral filamentous growth resembling a test tube brush is most characteristic of which organism?
S. moniliformis
L. pneumophila
G. vaginalis
E. rhusiopathiae
Acid-fast staining of a smear prepared from a digested sputum shoed slender, slightly curved, beaded red mycobacterial rods. Growth on Middlebrook 7H10 slants produced buff-colored microcolonies with a serpentine pattern after 14days at 37oC. Niacin and nitrate reduction tests were positive. What is the most probable presumptive identification?
Mycobacterium tuberculosis
Mycobacterium kansasii
Mycobacterium ulcerans
Mycobacterium avium complex
MPT 64 ANTIGEN detection is for rapid confirmation of:
S. aureus
M. tuberculosis
N. gonorrhoeae
T. pallidum
Which one of the following cannot be cultivated on artificial cell-free media?
F. tularensis
H. pylori
M. leprae
V. cholerae
Associated with patients with AIDS is most characteristic of:
M. marinum
M. avium-intracellulare
The ability to grow in MacConkey is a characteristic of which species of mycobacteria?
M. avium complex
M. fortuitum
M. tuberculosis
A gram-positive branching filamentous organism recovered form a sputum specimen was fount to be positive with modified acid-fast stain method. What is the most likely presumptive identification?
corynebacterium spp.
Nocardia spp.
Which of the following would be negative using acid-fast stain?
Cryptosporidium sp.
Actinomyces israelii
Isospora belli
Nocardia asteroides
TWAR strains of Chlamydia are associated with which type of infection?
Subacute bacterial endocarditis
Acute respiratory syndrome, pneumonia
Nongococcal urethritis
For ABSCESS, lesion, wound, pustule, and ulcer AEROBIC SWAB moistened with:
Stuart's medium
Amie's medium
Both of these
none of these
Common pH indicators used for O-F tests, and the color changes they undergo with acidic conditions, include:
Bromcresol purple, which changes from purple to yellow
Andrade's acid fuchsin indicator, which changes form pale yellow to pink
Phenol red, which changes from red to yellow
Bromthymol blue, which changes from green to yellow
all of these
Which of the following produces macroconidia that are large, multicellular, and club-shaped with smooth walls?
fonsecaea pedrosoi
epidermophyton floccosum
microsporum audouinii
It is characterized by MIXED SPORULATION (cladosporium, acrotheca, phialophora, with one usually predominant):
Fonsecaea
Phialophora
Cladosporium
Viruses contain:
both DNA and RNA
neither DNA or RNA
either DNA or RNA
polysaccharide shell
Persons can be infected by HTLV via:
sexual contact
breast milk
both of these
The fecal specimen is best collected in:
plastic container with tight-fitting lid
waxed cardboard
both of these
none of these
Concentration technique useful in the recovery of both helminth eggs and protozoan cysts:
Acid ether concentration technique (AECT)
Formalin ether concentration technique (FECT)
Sheather's sugar flotation
all of these
Smears are used in the demonstration of microfilaria and rapid diagnosis of malarial infection.
Thick smears
Thin smears
Smears are most useful in species IDENTIFICATION of malarial parasited.
Thick smears
Thin smears
Which of the following does NOT ordinarily produce egg in feces:
A. duodenale
a. lumbricoides
S. stercoralis
N. americanus
The usual method of infection for hookworms and Strongyloides stercoralis:
ingestion
penetration through skin
vector
infected meat
The adult Spirometra sp. (sparganosis) are usually mistaken for adult:
Diphyllobothrium latum
Hymenolepis nana
Taenia saginata
Injection of ___ through the genital pore of Taenia sp. will help one make an accurate count of the lateral branches of the uterus.
Methylene blue
India ink
Iodine
Which of the following would have a double-walled, wrinkled cyst form?
Naegleria fowleri
Enramoeba hartmanni
Acanthamoeba castellanii
Leishmaniasis is transmitted by the bite of infected female phlebotomine sandflies. The sandflies inject the infective stage during blood meals:
Amastigote
Promastigote
Epimastigote
Trypomastigote
For Plasmodium malariae, the period between attacks is:
24 hours
48 hours
72 hours
NOT ALL STAGE of the parasite occur in peripheral blood in infections with:
P. malariae
P. vivax
P. falciparum
P. ovale
It is produced by both sexual and asexual stages and can distinguish between P. falciparum and non- P. falciparum but not between the non-P. falciparum species:
plasmodium LDH
histidine rich protein II (HRP II)
AUTOFLUORESCENCE is characteristic of this organism:
Entamoeba histolytica
trichomonas vaginalis
Cyclospora cayetanensis
Cryptosporidium parvum
