Which is NOT a step in Cold Autoadsorption?

—usually directed toward Rh antigens , especially ‘e’ antigen

patient is usually ‘e’ positive and has a positive DAT

performed when the DAT is positive

Elution Methods

reduces pH causing Ag-Ab dissociation

Elution Methods

denature antigens by dissolving the cell membrane lipid bilayer

Elution Methods

causes conformational changes in the RBC membrane and the antibody molecules

Elution Methods

lyses RBCs to break Ag-Ab bonds

patient’s serum, + patient’s red blood cells + enhancement carried through the IAT procedure

patient’s red blood cells are tested for sensitization with IgG or complement using AHG

removal of warm autoantibodies ONLY to determine whether an underlying alloantibody exists

pretreat the patient’s RBCs with DTT and enzymes to remove as much in vivo attached antibody as possible

recently transfused patients

—adsorption uses RBCs that either match the patient’s phenotype or have a combination of antigens that “selectively” remove certain known antibody specificities

Antibody or antigen fixed to a microwell plate

filter-unagglutinated cells pass through; agglutinated cells cannot

Reasons to perform the IAT?

If an autoantibody is hiding an alloantibody what procedure is needed?

auto anti- e

anti-I and anti-H

auto anti-P

positive DAT, but eluate testing is negative

could be _______