WorksheetsMOLBIO FINALS PLATINGS
Total questions: 65
Worksheet time: 33mins
Cuvette of choice for measurement of proteins
Aluminum
Plastic
Quartz
Glass
It is a gel free technique that has an advantage due to its superior separation efficiency, small sample consumption and short analysis time.
Capillary Electrophoresis
SDS-PAGE
HPLC
2D-GE
Polymerized acrylamide (polyacrylamide) forms a mesh-like matrix suitable for the separation of proteins of typical size. The strength of the gel allows easy handling.
Both statements are true
Both statements are false
The first statement is false and the second statement is true
The first statement is true and the second statement is false
In Western blot technique, the protein is specifically detected by reacting it with:
Nitrocellulose
Labeled antibody
Antigen
NaCl
The end color of Lowry Method if positive for protein
Purple color
Brown
Red
Blue-green color
Antibodies not only protect us from infections, they can also be used in laboratories to determine whether or not a patient has or has had a disease.
True
False
Sandwich ELISAs are particularly suited to the analysis of complex samples, since the antigen does not need to be purified prior to the assay yet still delivers high sensitivity and specificity
TRUE
FALSE
PNPP (p-Nitrophenyl Phosphate, Disodium Salt) is a widely used substrate for detecting alkaline phosphatase in ELISA applications. When alkaline phosphatase and PNPP are reacted, a yellow water-soluble reaction product is formed.
The first statement is true and the second statement is false
The first statement is false and the second statement is true
Both statements are true
Both statements are false
This is best for when analyzing the immune response to an Ag:
Sandwich ELISA
Indirect ELISA
Direct ELISA
Inhibition ELISA
The detection antibody can be enzyme conjugated, in which case this is referred to as a direct sandwich ELISA.
TRUE
FALSE
This colorimetric assay is read at 562nm.
Biuret
Lowry
BCA
Bradford
Place the following steps in MALDI-TOF in their correct sequence.
1 Vaporized ions travel towards the detector.
2 Sample is vaporized
3 The matrix absorbs the ultraviolet light and converts it to heat energy
4 The sample for MALDI is uniformly mixed in a large quantity of matrix.
4321
2413
3214
4132
NPP hydrolysis Thermo Scientific Pierce PNPP (p-nitrophenyl phosphate) is a colorimetric, soluble substrate of alkaline phosphatase for use in ELISA applications.
TRUE
FALSE
The most popular method used with proteomic analysis.
Capillary HPLC
Reverse phase HPLC
Affinity HPLC
Ligand exchange HPLC
Affinity chromatography relies on
Ligand binding abilities
Mass
Size
Charge
Intercept (PBS) Blocking Buffer is the blocking buffer of choice for near-infrared fluorescent applications when using a phosphate-buffered saline (PBS) system. Contains no mammalian proteins for the lowest cross-reactivity with mammalian antibodies. Excellent choice for biotin-streptavidin detection.
Both statements are true
Both statements are false
The first statement is true and the second statement is false
The first statement is false and the second statement is true
SDS-PAGE is an analytical technique to separate proteins based on their molecular weight.
TRUE
FALSE
The procedure for a sandwich ELISA firstly requires the well of an ELISA plate to be coated with a:
CAPTURE Ab
IGM
IgG
Detection Ab
Bovine serum albumin (BSA) is a globular protein (~66 kDa MW) that is used in numerous biochemical applications due to its stability and lack of interference with biological reactions.
TRUE
FALSE
Third step in a Two-dimensional electrophoresis
SDS-PAGE
Isoelectric focusing
Spot Visualization
MS Analysis
Sequence alignment helps scientists to
infer the functions of newly synthesized genes
predict new members of gene families
all of the above
trace out evolutionary relationships
This is a unique identifier given to a DNA or protein sequence record to allow tracking of different versions of that sequence and the associated sequence overtime in a single data repository
Accession number
Score
GI number
Expect value
You have sequenced five PCR products of DNA samples of Escherichia coli you have isolated from clinical specimens. You want to determine in which nucleotide bases the five DNA sequences vary, which tool will be most helpful to you?
MUSCLE
PrimerBlast
EMBOSS Water
BLAST
When you search GenBank for the DNA sequence of gene X, you will find a link called FASTA. This refers to
the coding region of the gene
the series of numbers assigned to each sequence record processed by NCBI
nucleotide bases that code for a protein
a nucleic acid or protein sequence represented by single-letter codes
Which of the following is a nucleotide sequence database?
SWISS PROT
EMBL
PDBj
TREMBL
In pairwise sequence alignments, gaps are more often seen in Local alignments than in global alignments.
FALSE
TRUE
When you get a BLAST result for a DNA sequence, the Graphic Summary refers to
a genetic map that shows the details of gene location relative to another gene in the chromosome.
a diagram representing evolutionary relationships among organisms.
The experimental three-dimensional structure of a protein
the red bars specifying sequences found in the database that aligns to your sequence.
The alignment procedure that tries to align regions with high level of matches without considering the alignment of the rest of the sequences
multiple sequence alignment
global alignment
pairwise alignment
local alignment
(Image Alignment) An example of global sequence alignment:
B
A
C
It is a diagram representing evolutionary relationships among organisms
Chromatogram
Phylogenetic tree
Pedigree
Algorithm
In a sequence alignment, a dot between matched bases indicates
the bases are conserved across all members of a species
a gap in the sequence
identical bases
non-identical bases
If you are studying a set of all RNA molecules in a mouse, you are dealing with
Metabolomics
Transcriptomics
Proteomics
GENOMICS
This is the process of identifying locations of genes, all the protein coding regions, and the regulatory sequences in a genome.
Sequence alignment
Genome annotation
DNA barcoding
Genotyping
If you want to compare the amino acid sequence of your protein with those that are stored in the databases, which tool will you use?
BLASTp
MUSCLE
EMBOSS
BLASTn
If you want to examine the three-dimensional structure of a protein, you will look into which database?
PDBe
Ensembl
GenBank
Signal transduction databases
How many primers are needed for a successful PCR of a gene when the only information available is the protein amino acid sequence?
3
2
2
More than four
Which is true about the reverse primer?
It is the complement of the 3-prime end of the sense DNA template.
It has the sequence of that found at the end of the DNA target.
It is read as 3-prime to 5-prime sequence.
It is the complement of 5-prime end of the sense DNA template
The primers used in polymerase chain reaction are
Single-stranded RNA oligonucleotide
Double-stranded DNA oligonucleotide
Double-stranded RNA oligonucleotide
Single-stranded DNA oligonucleotide
Which double-stranded DNA molecule has the highest melting temperature?
A DNA polymer of 100,000 base pairs
An oligonucleotide with a large number of repeating C-G-C codons
A molecule of 5,000 bps with a high number of A-T base pairs
An oligonucleotide with a repeating sequence of A-A-A at the 5-prime end.
If the melting temperature of a pair of primers is calculated as 62 C, at what annealing temperature would you set your PCR cycling?
52 C
57 C
67 C
72 C
Which of the following may result in no band or faint band in agarose gel electrophoresis?
The annealing temperature is too high
Too much primers added
Too many cycles used
The melting temperature is very low
Primer annealing means
The binding of the primer to the template DNA.
The extension of the DNA strand starting from the primers.
The conversion of mRNA to cDNA
The separation of template DNA strands.
How many primers are needed for a successful PCR of a known sequence of a gene?
4
2
1
3
If the primer sequence is TCG, which section of the DNA will it anneal to?
AGC
GAC
ATC
CAT
What is the function of a primer?
to copy DNA
to identify the particular region of the DNA to be copied by PCR
to create DNA nucleotides
to maintain the temperature of the PCR reaction
Which of the following is the LEAST factor to consider when optimizing a new primer pair?
Annealing temperature
Number of cycles
Mg ion concentration
Denaturation temperature
How many primers are needed for a successful PCR of a gene that is common among different species?
2
1
4
3
What is the effect of homology between the three-prime ends of the forward and reverse primer?
Formation of primer dimer
Formation of more non-specific products
Enhanced binding between the primers and their respective templates
Formation of hairpin structure
What would be the expected effect on a PCR reaction if the primers used were slightly shorter and more variable than the intended oligonucleotide sequences?
The PCR reaction would not commence.
The PCR reaction would end after one cycle.
The reaction would generate single short PCR product.
The reaction would yield a mixture of non-specific products.
What type of PCR primers are designed when the only information available is the protein amino acid sequence?
mRNA primers
Universal primers
Target-specific
Degenerate primers
Principle involved in MALDI – TOF MS
Chemiluminiscence
Ionization
A and B
Neither A nor B
Gene target that may be used for diagnosing infection / condition associated to HPV16 or HPV18
E6
E7
A and B
Neither A nor B
Specimen/s that may be used for the molecular detection of Rickettsia species:
Eschar swab
Eschar biopsy
A and B
Neither A nor B
Toxin genes are the main targets of real – time PCR for the diagnosis of infection caused by:
Staphylococcus aureus
Streptococcus pneumoniae
A and B
Neither A nor B
Gene target for bacterial infection using molecular techniques include/s:
16s rRNA
23s rRNA
A and B
Neither A nor B
Most widely applied NAAT for the diagnosis of Mycoplasma pneumoniae is:
Southern Blot
PCR
A and B
Neither A nor B
Advantage/s of using whole genome sequencing (WGS) for detecting MRSA
Virulence of the strain may be assessed
Prediction of resistance is possible
A and B
Neither A nor B
Screening using molecular techniques are done to pregnant individuals in order to detect the possibility of colonization associated to which pathogen?
Streptococcus agalactiae
Group A Streptococcus
A and B
Neither A nor B
Which of the following is extracted for MALDI – TOF MS microbial identification?
Nucleic acid
Protein
A and B
Neither A nor B
Confirmatory assay for the diagnosis of HIV infection
Southern Blot
Western Blot
A and B
Neither A nor B
Advantage/s of using molecular techniques for diagnosing infectious diseases:
Rapid results are produced compared with the classical microbiological testing
Different strains may be identified
A and B
Neither A nor B
DNA target/s for the molecular detection of fungal infection
rDNA
Cytochrome B
A and B
Neither A nor B
Arthropod – Borne bacterial infection that may be diagnosed thru PCR based assays
Borellia species
Chlamydia trachomatis
A and B
Neither A nor B
In order to achieve a purified protein extract for MALDI – TOF MS, which of the following is commonly added along with the matrix?
Formic acid
Picric acid
A and B
Neither A nor B
Molecular technique/s used for the diagnosis of fungal infection:
NAATs
ISH
A and B
Neither A nor B
