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Bioprocess Technology

Total questions: 25

Worksheet time: 13mins

Name
Class
Date
1.

Downstream process includes

a)

Fermentation Media Selection

b)

Strain Selection

c)

Cell separation

d)

Product recovery

2.

Why the fermentation media selection is an important aspect for industrial production purpose?

a)

to ensure strain improvement

b)

Low cost product delivery

c)

Improve in downstream section

d)

All of the above

3.

Propagation media is used in

a)

Fermentor

b)

Starter Culture

c)

Seed tank

d)

All of these

4.

Primary recovery depends upon " intracellular or extra cellular product formation"

a)

true

b)

false

5.

Yeast feeds on this element in order to give off CO2 gas.

a)

sugar

b)

vinegar

c)

salt

6.
Fermentation involves
a)
aerobic respiration
b)
anaerobic respiration 
7.

Fermentation leads to the improvement of _____________ in the final product

a)

Taste

b)

Quantity

c)

pH

d)

Amino acids

8.

Aspergillus spp. breaks down the protein into ____________

a)

Carbohydrates

b)

Sugar

c)

Amino acid

d)

Lipids

9.

Q.1: Screening of microorganisms includes .

a)

Done in one or few steps

b)

Allow discarding of many valueless microbes

c)

Easy detection of the small percentage of useful microorganism

d)

All of the above

10.

Primary screening of organic acids or amines producing organisms is done by

a)

Crowded plate technique

b)

Dye such as neutral red or bromothymol blue is used

c)

Enrichment culture Technique

d)

Auxanographic method

11.

Antibiotic producing microorganisms can be detected by the method

a)

Incorporation of CaCO3 in the agar medium

b)

Crowded plate method

c)

Indicator Dye Method

d)

None of the above

12.

Secondary screening can give information about ...........

a)

The genetic instability in microbial cultures

b)

Number of products produced in a single fermentation

c)

The structure of product

d)

All of the above

13.

Yeast is activated by what element?

a)

warmth

b)

cold

c)

fire

14.

Bioreactor is also known as

a)

Fermentor

b)

Vessel

c)

Fermentation

15.

What are the enzymes are used in microbial enzyme

a)

Protease

b)

Lipase

c)

Both (a) and (b)

d)

None of the above

16.

Bioreactor are used as

a)

High level of pH

b)

Low level of pH

c)

Temperature control

d)

Both a and b

17.

Example of Primary metabolites

a)

Lactic acid

b)

Ethanol

c)

Both a and b

d)

None of the above

18.

Fermenter allow the production of material in

a)

small amount

b)

fixed amount

c)

bulk amount

d)

variable amount

19.

What is the basic function of the fermenter?

a)

To sterilize the medium

b)

To recover the product

c)

To purify the product

d)

To provide optimum growth conditions to organisms and obtain the desired product

20.

Which of the following material is preferable for the construction of small-scale fermenter?

a)

Quartz

b)

Glass

c)

Iron steel

d)

Wood

21.

The Batch culture is a/an ______ culture system.

a)

Open

b)

Closed

c)

Isolated

d)

Semi-closed

22.

A period during which the growth rate of cells gradually increases is known as _____

a)

Lag phase

b)

Log phase

c)

Stationary phase

d)

Deceleration phase

23.

The Yield Factor (Y) does not vary upon which of the following?

a)

pH

b)

Growth rate

c)

Temperature

d)

Amount of enzyme

24.

The batch culture or fermentation can be used to produce ________

a)

Organic acids

b)

Amino acids

c)

Single Cell Protein

d)

Antibiotics

25.

The following figure shows the growth of typical microbial culture in batch conditions. What is A, B, C and D?

a)

A-lag phase, B-log phase, C-stationary phase, D-deceleration

b)

A-log phase, B-lag phase, C-stationary phase, D-deceleration

c)

A-deceleration, B-log phase, C-lag phase, D-stationary

d)

A-lag phase, B-log phase, C-deceleration, D-stationary