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WorksheetsPRACTICAL 2- CITRA COURSE LMFF1512 (PCR-RFLP)
Total questions: 10
Worksheet time: 2mins
PCR-RFLP is abbreviation for?
Polymerase Chain Reaction-Restoration Fragment Length Polymorphism
Polymerase Chain Reaction-Restriction Fragment Length Polymorphism
Polymerase Chain Reaction-Reverse Fragment Length Polymorphism
What step should we do before PCR?
Agarose gel electrophoresis
Restriction enzyme digestion
Analysis
DNA extraction
These are steps in PCR process, EXCEPT
Elongation
Denaturation
Annealing
Extraction
In PCR, this step involve separation of DNA template into single strands.
Elongation
Final extension
Elongation
Denaturation
These are applications for RFLP analysis, EXCEPT
DNA fingerprinting
Genetic diversity
Protein identification
Paternity
These equipment are used in PCR-RFLP experiment, EXCEPT...
Spectrophotometer
Thermal cycler
PCR machine
Waterbath
What chemical is used to stain a gel in electrophoresis?
TAE buffer
Ethidium bromide
Loading dye
100 bp ladder
Which of the instrument is used to view band in a gel?
Gel documentation system
Thermal cycler
Vortexer
Waterbath
A few bloodstains were found at a crime scene. A blood sample was obtained from Suspect 1, Suspect 2 and a victim. All bloodstains and blood samples were sent to a lab for PCR-RFLP analysis. From the result, the bloodstains belongs to...
Suspect 1
Suspect 2
Victim
Suspect 1 & 2
A bloodstain was found at a crime scene. Two suspects were identified. Blood was obtained from both suspect and a victim for PCR-RFLP analysis. From the result, the bloodstain belongs to...
Suspect 1
Suspect 2
Victim
Suspect 1 & 2
