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WorksheetsSequencing Technologies BTN602C Genomics and Proteomics
Total questions: 13
Worksheet time: 10mins
Which part of Sanger (Cycle) Sequencing is NOT found in regular Polymerase Chain Reaction (PCR)?
DNA polymerase
Primers
Deoxyribonucleotides (dNTPs)
Dideoxyribonucleotides (ddNTPs)
DNA template
How are deoxyribonucleotides different from dideoxyribonucleotides? Choose all that apply.
Deoxyribonucleotides are used in both PCR and Cycle Sequencing.
Dideoxyribonucleotides are missing an OH group compared to deoxyribonucleotides.
You cannot add new nucleotides to a deoxyribonucleotide.
Deoxyribonucleotides are NOT found in natural living cells.
Dideoxyribonucleotides are labeled with fluorescent tags.
How is DNA synthesis in PCR and Cycle Sequencing the SAME?
Both require DNA polymerase.
Both require primers.
Both require deoxynucleotides (dNTPs).
Both require dideoxynucleotides (dNTPs).
Both generate new DNA products of equal length.
Place the steps of DNA Sequencing below into their correct order.
A. Detection of fluorescence w/ lasers & sensors
B. PCR w/ fluorescent dideoxynucleotides
C. Separate DNA by size w/ gel electrophoresis
D. Match DNA sequence using BLAST database
A, B, C, D
B, C, A, D
B, A, C, D
D, B, C, A
Which of the following is chemical nucleotide sequencing method
Sanger method
Maxam Gilbert method
Edmans method
Automated sequencing method
The principle of Sanger's method relies on
use of chemicals for base specific cleavage
use of dNTPs for chain termination
use of ddNTPs for chain termination
Use of p32 for chain termination
Automated DNA sequencing is an improvement of Sanger's method where
ddNTPs are used for chain termination
PCR is used for making sequencing
Fluorescently labelled dNTPs are used for chain termination
Fluorescently labelled ddNTPs are used for chain termination
NGS consists of the following steps in sequence
library construction, amplification, sequencing
sequencing, library construction, amplification
Amplification, Sequencing and library construction
none of the above
Emulsion PCR refers to
amplification of DNA in water
amplification of DNA in organic solvent
amplification of DNA in water in oil droplets
synthesis of DNA in water
problems with 454
repeat regions cant be read
cant be used for large genomes
allows in depth sequencing
short stretches are read
luciferase uses the substrate
apyrase
luciferin
oxyluciferin
Pyrophosphate
emulsion PCR can be linked to
Solid sequencing
illumina sequencing
454 sequencing
automated sanger sequencing
Which of the following is a feature of the Illumina sequencing method?
It generates long reads
It is a high-throughput sequencing method
It is a single-molecule sequencing method
It has a high error rate
