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Comprehensive Course for Biotechnologists-A & B Section

Total questions: 40

Worksheet time: 30mins

Name
Class
Date
1.

The complete transfer of one or more electrons from outermost valence shell of one atom to another atom.

a)

Hydrogen bonding

b)

Vander Waal’s interactions

c)

Ionic bond

d)

Hydrophobic interactions

2.

This has only one chiral centre with different configuration.

a)

Epimers

b)

Anomers

c)

Isomers

d)

Enantiomers

3.

Which type of cell signalling involves distant sending and target cell?

a)

Paracrine

b)

Autocrine

c)

Endocrine

d)

Cell-cell contact

4.

This refers to the tendency of atoms to prefer to have eight electrons in the valence shell

a)

Octet rule

b)

Valence electron

c)

Electronegativity

d)

Lewis symbol

5.

Electron that is located on the outermost shell

a)

proton

b)

neutron

c)

electron

d)

valence electron

6.
a)

Molecular Formula

b)

Empirical Formula

c)

Structural Formula

7.
a)

ball and stick model

b)

space filling model

c)

empirical formula

d)

molecular formula

8.

Is a way of expressing in symbols the elements present in a compound, as well as the number of atoms of each element present in the compound.

a)

chemical formula

b)

chemical equation

c)

chemical reaction

d)

chemical distribution

9.

An atom's electronegativity depends on

a)

Nuclear charge

b)

Atomic radius

c)

Electron shielding

d)

All of these

10.

Non-polar covalent molecules are likely to be what at room temperature?

a)

Liquids

b)

Solids

c)

Gases

11.

Van der waals forces are found between

a)

Non polar molecules

b)

Polar molecules

c)

Ionic compounds

d)

Atoms

12.

Hydrogen bonding only takes place between hydrogen and

a)

Carbon, oxygen, fluorine

b)

Carbon, nitrogen, chlorine

c)

Nitrogen, oxygen, fluorine

d)

Carbon, nitrogen, fluorine

13.

How will you describe enzymes?

a)

Non-growth-related product

b)

Secondary metabolite

c)

Lag phase

d)

Growth related product

14.

Carbohydrates are commonly found as starch in plant storage organs. Which of the

following properties of starch make it useful as a storage material?

a. Easily translocated

b. Chemically non-reactive

c. Easily digested by animals

d. Osmotically inactive

e. Synthesized during photosynthesis

The useful properties are

a)

(1), (3) and (5)

b)

(1) and (5)

c)

(2) and (3)

d)

(2) and (4)

15.

Thickness of plasma membrane is

a)

50 Å

b)

50 Å – 75 Å

c)

75 Å - 100 Å

d)

100 Å - 175 Å

16.

The hydrolytic enzymes which occur in lysosome work most effectively under

condition of:

a)

High pH

b)

Low pH

c)

Neutral

d)

Salinity

17.

Conditions required for successful activation of T helper

a)

Presence of signal 0

b)

Presence of inadequate amount of CD28

c)

Presence of CD80 or CD86 in APC

d)

Formation of microclusters of TCR complex in T helper

18.

Examples for professional APCs

a)

T lymphocytes

b)

Dendritic cells

c)

B lymphocytes

d)

Erythrocytes

19.

The first recombinant DNA molecule was produced by

a)

Herb Boyer

b)

Paul Berg

c)

Stanley Cohen

d)

Herb Boyer and Stanley Cohen

20.

Recombinant DNA is also called as

a)

Chimera

b)

Plasmid

c)

Vector

d)

None of the above

21.

GAATTC is recognition site of restriction endonucleases

a)

Hind III

b)

EcoRI

c)

Bam I

d)

Hae III

22.

Which type of restriction enzymes are commonly used in rDNA technology

a)

Type I

b)

Type II

c)

Type III

d)

Type IV

23.

Which of the following statements are true regarding r DNA technology

a)

rDNA technology is used to obtain large number of copies of specific DNA fragments

b)

rDNA technology is used to obtain large quantities of the protein produced by the concerned gene

c)

rDNA technology is used to integrate gene of interest into chromosomes where it expresses itself

d)

All of these

24.

The ends of DNA fragments are sticky due to

a)

unpaired bases

b)

free methylation

c)

endonuclease

d)

calcium ions

25.

Which of the following enzymes separates the two strands of DNA during replication?

a)

Gyrase

b)

Topoisomerase

c)

Helicase

d)

DNA polymerase

26.

Which of the following reactions is required for proofreading during DNA replication by DNA polymerase III?

a)

5’ to 3’ exonuclease activity

b)

3’ to 5’ exonuclease activity

c)

3’ to 5’ endonuclease activity

d)

5’ to 3’ endonuclease activity

27.

DNA replication is bidirectional and anti-parallel. Which of the statement is FALSE regarding the DNA replication?

a)

The DNA synthesis i.e. addition of nucleotide occurs from 5'-3' position

b)

The DNA synthesis is semi-continuous with continuous leading strand and discontinuous lagging strand.

c)

The synthesis of leading and lagging strands occurs simultaneously

d)

None of the Above

28.

Autoimmune disorders are more common in women than men

a)

True

b)

False

29.

A 25-year-old female is admitted to the ER in anaphylactic shock due to a bee sting. According to the patient's mother, the patient is severely allergic to bees and was recently stung by one. This type of anaphylactic reaction is known as a?

a)

Type III Hypersensivity Reaction

b)

Type IV Hypersensivity Reaction

c)

Type II Hypersensivity Reaction

d)

Type I Hypersensivity Reaction

30.

In which type of hypersensitivity reactions do immune complexes form and deposit into tissues triggering an acute inflammatory response?

a)

Type 1

b)

Type 2

c)

Type 3

d)

Type 4

31.

Which type of hypersensitivity involves IgE?

a)

Type I

b)

Type II

c)

Type III

d)

Type IV

32.

Which type of hypersensitivity involves T cells?

a)

Type I

b)

Type II

c)

Type III

d)

Type IV

33.
Major histocompatibility complex is also known as _______.
a)
H-2
b)
Human Leukocyte Antigen
c)
Tissue typing
d)
None of the above
34.
T cells recognize only ____ presented via MHC by antigen presenting cells.
a)
peptides
b)
proteins
c)
carbohydrates
d)
antigens
35.

Which of the following proteins have not been used in genome editing?

a)

ZFN

b)

TALENs

c)

CRISPR-Cas9

d)

MHC

36.

If a length of DNA has 45000 base pairs, how many complete turns will the DNA

molecule take?

a)

4500

b)

45000

c)

45

d)

450

37.

Mesosome in a bacterial cell wall is

a)

Plasmid

b)

Connection between two cells

c)

Plasma membrane infolded for respiration

d)

Ribosome

38.

The enzymes of TCA-cycle for complete oxidation of Pyruvic acid into CO2 and

H2O are present in

a)

Chloroplasts

b)

Mitochondria

c)

Peroxisomes

d)

Mesosomes

39.

Which one of the following is an example of glycoprotein?

a)

Haemoglobin

b)

Lecithin

c)

Mucin

d)

Casein

40.

The CRISPR-Cas9 system in bacteria is akin to our body’s

a)

Digestive system

b)

Immune system

c)

Circulatory system

d)

Respiratory system

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