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WorksheetsHISTOPATHOLOGY LAB SAFETY PRELIM REVIEWER PART 1
Total questions: 65
Worksheet time: 58mins
Art of analyzing and interpreting the shapes, sizes and architectural patterns of cells and tissues within a given specific clinical background
(a)
The art and science performed by the histotechnologist to produce a tissue section of good quality
(a)
Responsible for the processing of tissue sections
(a)
Provide the basic concepts about the principles and technicalities involved in histopathologic procedures. Provide skills in tissue preparation from fresh to properly mounted specimen
(a)
It is one of the important machines required in histopathology for processing organ and tissues before being embedded in paraffin wax
(a)
The older version or manual method of automatic tissue processor
(a)
An instrument where tissues are cut into sections
(a)
process of cutting into thin sections
(a)
What do you call about the thin sections of tissue
(a)
Used when the specimen is subjected for the frozen section. A a refrigerated apparatus used in fresh tissue microtomy, for freezing the tissue into the block holder to correct degree of hardness to facilitate easier and faster sectioning
(a)
The cryostat is an apparatus used to keptt inside the cold chamber which has been maintained at a
5 to 25 C
20 to 35 C
-5 to - 30 C
-15 to -25 C
The average temperature of the Cryostat is
- 20 C
- 15 C
25 C
30 C
Used to float paraffin ribbons, to stretch sections and remove wrinkle
(a)
The temperature of the tissue water bath should be :
10 degrees below the melting point of paraffin
25 degrees below the melting point of paraffin
10 degrees above the melting point of paraffin
25 degrees below the freezing point of paraffin
What is the approximate temperature of tissue water bath?
30 - 45 C
45 - 50 C
50 - 55 C
60 - 75 C
Used for drying or dehydrating of paraffin ribbons after the water bath. Removing moisture from samples
(a)
Optimizes times during stain process to maximize efficiency (fast and increase accuracy) in obtention of results for a timely diagnosis
(a)
An equipment that is used by both the pathologist and the histotechnologist. Pathologist examines the slide under the microscope to identify a disease process or an abnormality that will directly affect the patient's treatment
(a)
A microscope must accomplish these things:
It must magnify the object
It must resolve the details of the object
It must make these details visible
It must dry and extract all the samples at once
Microscope with more than one lens and its own light source. There are ocular lenses in the binocular eyepieces and objective lenses in a rotating nosepiece closer to the specimen
(a)
Only uses one eyepiece when viewing the specimen. They are light weight and are inexpensive
(a)
Have two eyepieces and are more convenient and comfortable to use. It is the most common choice.
(a)
Have a third eyepiece tube that can be used by another person simultaneously or by an LCD camera. More expensive than the other two types.
(a)
Provides support for the microscope. should be large and solid enough to allow the microscope to stand by itself
(a)
Supports and holds the magnifying and adjustment system. It can be used as a handle for carrying the microscope.
(a)
Is the flat platform where the slide is placed for examination.
(a)
Is located directly under the stage and holds the condenser and diaphragh
(a)
Permits movement of the stage while holding the slide in the phase of focus
(a)
Is located at the end of the body tube for holding the objectives
(a)
Consist of a system of lenses located at the end of the body tube that is held in place by the nosepiece and is closer to the slide under examination. The purpose of the objective is to increase or decrease magnification
(a)
What do you call when one objective is focused on the turret, all lenses will be approximately in focus
(a)
Is the distance between outer lens of objective and the cover glass of the slide under examination
(a)
the process that increases the size of the structure under examination
(a)
The total magnification of a microscope is the product of the magnifying power of the objective and eyepiece, with a normal tube length of 180 mm
True
False
Process of ensuring and maintaining personal as well as environmental health and safety in the laboratory
(a)
The first step is to identify all electrical, mechanical and biological hazards that can potentially cause harm in the laboratory
True
False
Ratio of the sodium hypochlorite/ bleach
(a)
The “lab standard” applies to the laboratory use of chemicals and mandates written in the Material Safety Data Sheet (MSDS) that address the particular hazards and precautions required for safe use
True
False
A regulatory limit on the amount or concentration of a substance in the air. This is usually based on an eight-hour time weighted average
PEL
TLV
OEL
MSDS
o Represents the maximum level of an airborne substance that a worker can be safely subjected to without being susceptible to harm or injury
(a)
Indicates the level of admissible exposure, for a length of time (usually 8 hours), to a chemical or physical hazard that is not likely to affect the health of a worker
(a)
Every chemical should be labeled with certain basic information of the MSDS including:
Chemical name and, if a mixture, names of all ingredients
Date purchased or made
Expiration date, if known
Hazard warnings and safety procedures
name of person who sells the reagent
Are chemicals that cause reversible inflammatory effects at the site of contact with living tissue, especially the skin, eyes and respiratory passages
(a)
Cause destruction or irreversible alterations when exposed to living tissue, or destroy certain inanimate surfaces (generally metal)
(a)
Cause allergic reactions in some exposed workers, not just in hypersensitive individuals
(a)
Are substances that induce tumors, not only in experimental animals but also in humans
(a)
Which of the following are Carcinogenic chemicals
Chloroform
chromic acid
formaldehyde
basic fuchsin
Congo red
Which of the following are carcinogenic dyes
basic fuchsin
benzidine
g Congo red
diamino- benzidine
nickel chloride
Are capable of causing death by ingestion, skin contact or inhalation at certain specified concentrations
(a)
Which of the following are toxic materials
methanol
chromic acid
osmium tetroxide
uranyl nitrate
potassium dichromate
Slips and falls from working in wet locations and the ergonomic hazards of lifting, pushing, pulling, and repetitive tasks are example of what hazard?
(a)
Refer to anything that can cause disease in humans, regardless of their source
(a)
Allergens, are one of the most important health hazards, yet they are frequently overlooked
True
False
Degree to which healthcare services strive to provide accurate desired outcomes for patients and are consistent with current professional knowledge
(a)
“Freedom from accidental injury”
(a)
System of routine technical activities. Provides routine and consistent checks to identify, address errors and omissions, ensures data integrity, correctness and completeness and also records all quality control activities
(a)
Planned system of review procedures conducted by personnel not directly involved in the laboratory process
(a)
In QAP, They Mandate guidelines for histopathology laboratory
College of American Pathologists
United Kingdom National External Quality Assessment Service
OSHA
CDC
Stained preparations from departmental archival records are used to assess the quality of staining
(a)
Participating laboratories are asked to stain sections that have been submitted by the scheme organizer
(a)
Effective QMS
Skilled histotechnologist/ histotechnicians
Proper specimen collection
Low quality of reagents and equipment
Preventive Maintenance of equipment
Inefficient processing of results
Pre-analytical phase
Collection of the right specimen
The proper fixation of the specimen
The correct identification and transportation of the specimen
Procedure reliability using technical manual
Slides storage
Analytical Phase
Grossing
Procedure reliability using technical manual
Cutting of paraffin sections
The timely transportation of the specimen
Reports reaches the appropriate clinicians/surgeons
Post-Analytical Aspects
Diagnosis (hard copy) free of clerical errors
• Reports reaches the appropriate clinicians/surgeons
Filing of paraffin blocks
Staining
Slide labeling (mounting is included)
Which of the following organization mandates PEL, TLV, and OEL
OSHA
CDC
CAP
UK-NEQAS
