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MICROBIO

Total questions: 127

Worksheet time: 4hrs 14mins

Name
Class
Date
1.

Study of organisms too small to be seen by the naked eye.

(a)  

2.

Organisms which are too small to be seen by the naked eye

(a)  

3.

Two major categories of microbes:

a)

Cellular Microbes

b)

Acellular Microbes

c)

Granular Microbes

d)

Agranular Microbes

4.

(a)   (also called infectious particles), includes viruses and prions.

5.

(a)   (also called microorganisms), includes all bacteria, all archaea, all protozoa, some algae, and some fungi.

6.

Microbes that cause diseases.

(a)  

7.

Microbes that do not cause disease

(a)  

8.

Harmless microorganisms consist of the indigenous microflora that lives on or in the body. They cause disease only if they accidentally invade the “wrong place at the right time”.

(a)  

9.

Occur when opportunistic microorganisms gain access to a part

of the body where they do not belong.

(a)  

10.

What is the importance of microorganisms? (select all that applies)

a)

Microorganisms form the INDIGENOUS MICROFLORA of the human body.

b)

Some microorganisms are OPPORTUNISTIC PATHOGENS.

c)

Some microorganisms are SAPROPHYTES/DECOMPOSERS.

d)

Microorganisms are sources of food.

e)

Bacteria can be manipulated to produce enzymes and proteins they normally would not produce

11.

microflora Inhibit the growth of pathogens by :

(select all that applies)

a)

Occupying space

b)

Depleting food supply

c)

Secreting materials (waste products, toxins, antibiotics ) that prevent growth

of pathogens

d)

Nourishing the organisms and providing them energy.

12.

Organisms that live on dead or decaying organic matter. They aid in fertilization by returning inorganic nutrients to soil. They also break down dead and decaying organic matter into nutrients, phosphates and other chemicals for growth of plants.

(a)  

13.

Microorganisms are sources of food.

(select all that applies)

a)

Algae

b)

Plankton

c)

Phytoplankton

d)

Zooplankton

e)

Microplankton

14.

Bacteria synthesize chemicals that our body needs, but cannot synthesize.

a)

true

b)

false

15.

branch of microbiology dealing with plant-associated microbes and plant and animal diseases.

(a)  

16.

the manipulation (as through genetic engineering) of living organisms or their

components to produce useful usually commercial products (as pest resistant crops, new bacterial strains, or novel pharmaceuticals)

(a)  

17.

Study of composition and physiology of microbial communities in the environment

(a)  

18.

branch of medicine concerned with the prevention, diagnosis and treatment of

infectious diseases.

(a)  

19.

The insertion of foreign genes into microorganisms to enable the microorganisms to produce specific gene products or to enable them to be used for other purposes.

(a)  

20.

Study of the structure and functions of microbial cells.

(a)  

21.

The study of ancient microorganisms.

(a)  

22.

science based on the detection of risks associated with the production, manufacture and consumption of foods and water

(a)  

23.

concerned with microbial (bacterial, fungal, viral) diseases of domesticated

vertebrate animals (livestock, companion animals, fur-bearing animals, game,

poultry, fish) that supply food, other useful products or companionship.

(a)  

24.

Known as the "Father of microbiology", "Father of bacteriology" and "Father of protozoology". He was the first to see live bacteria and protozoa which he called animalcules by using single lens or simple microscope to view these microorganisms.

a)

Anton Van Leeuwenhoek

b)

Louis Pasteur

c)

Robert Koch

d)

Edward Jenner

25.

French chemist that;

▪ Disproved the theory of spontaneous generation or abiogenesis

▪ coined the terms aerobes and anaerobes

▪ Discovered Pasteurization

▪ Discovered silkworm disease, various vaccines (rabies, anthrax), process of alcohol

fermentation

▪ Germ theory of disease

a)

Anton Van Leeuwenhoek

b)

Louis Pasteur

c)

Robert Koch

d)

Edward Jenner

26.

German physician that;

▪ Discovered: Anthrax bacillus, M. tuberculosis, V. cholerae

▪ able to obtain pure cultures of bacteria using the petri dish (R.J. Petri) and Agar

as solidifying agent

▪ Tuberculin skin test

a)

Anton Van Leeuwenhoek

b)

Louis Pasteur

c)

Robert Koch

d)

Edward Jenner

27.

contributed to the development of the first vaccine which was against smallpox

a)

Anton Van Leeuwenhoek

b)

Louis Pasteur

c)

Robert Koch

d)

Edward Jenner

28.

discovered the cell when he looked at a sliver of cork through a microscope lens

a)

Robert Hooke

b)

Joseph Lister

c)

Paul Ehrlich

d)

Hans Chritian Gram

29.

pioneer of antiseptic surgery

a)

Robert Hooke

b)

Joseph Lister

c)

Hans Chritian Gram

d)

Hans Chritian Gram

30.

credited with finding the cure (salvarsan) for syphilis

a)

Robert Hooke

b)

Joseph Lister

c)

Paul Ehrlich

d)

Hans Chritian Gram

31.

a Danish bacteriologist noted for his development of the Gram stain

a)

Robert Hooke

b)

Joseph Lister

c)

Paul Ehrlich

d)

Hans Chritian Gram

32.

includes plants and animals that float along at the mercy of the sea's tides and

currents. Their name comes from the Greek meaning "drifter" or "wanderer."

(a)  

33.

results when a person ingests a toxin (poisonous substance) that has been produced by a microbe.

(a)  

34.

used to express the sizes of microorganisms.

(a)  

35.

the basic unit of length in the metric system.

(a)  

36.

The size of RBC's, bacteria and protozoa is measured as (a)  

37.

The size of viruses is measured as (a)   .

38.

A tiny ruler within the eyepiece of a compound light microscope, which is used to measure the sizes of cellular microorganisms.

(a)  

39.

an optical instrument that is used to observe tiny objects, that cannot be seen at all with the unaided human eye.

(a)  

40.

A (a)   contains only one magnifying lens.

41.

A (a)   contains more than one magnifying lens. It uses visible light as the source of illumination.

42.

Total magnification of the compound light microscope is calculated by multiplying the magnifying power of the ocular lens by the magnifying power of the objective being used.

a)

true

b)

false

43.

Credited for developing the first compound microscope.

(a)  

44.

He used compound microscopes with three lens systems.

(a)  

45.

He authored, Micrographia, a 1665 volume documenting experiments he had

made with a microscope. He coined the term "cell" while discussing the structure of cork.

(a)  

46.

The capacity of a microscope to distinguish or separate two adjacent

objects or points from one another.

(a)  

47.

an instrument that uses visible light and magnifying lenses to examine small objects not visible to the naked eye, or in finer detail than the naked eye allows.

(a)  

48.

Microscope where objects are observed against a bright background. Has Maximum magnification: 1000x, Resolution: 0.2um

(a)  

49.

Microscope where Illuminated objects are seen against a dark background. Has Resolving power: 0.2000um, Useful magnification: 1000x.

(a)  

50.

Microscope that uses the differences in the phase of light transmitted or reflected by a specimen to form

distinct, contrasting images of different parts of the specimen.

(a)  

51.

Compound microscope with an ultraviolet radiation source. Often used in immunology laboratories to demonstrate that antibodies stained with a fluorescent dye have combined with specific antigens; this is a type of immunodiagnostic procedure.

(a)  

52.

Couples a laser light source to a light microscope. A laser beam is bounced off a mirror that directs the beam through a scanning device. Cells are often stained with fluorescent dyes to make them more visible.

(a)  

53.

Uses an electron beam as a source of illumination and magnets to focus the beam. Only for dead organisms.

(a)  

54.

Type of Electron Microscope which allows examination of cellular and viral

Ultrastructure. Reveals the finest detailed structure of cells and viruses.

• Resolving power: 0.2nm

• Useful magnification: 200,000x

(a)  

55.

Type of Electron Microscope which is designed to create an extremely detailed

3D view of the specimen. Useful for examining surface features of cells and viruses/

• Resolving power: 20nm

• Useful magnification: 10,000x

(a)  

56.

Microscope that measures surface features by moving a sharp probe over the object’s surface. It enables scientists to view atoms or molecules on the surface of a specimen.

examples,

• Scanning Tunneling Microscope

• Atomic Force Microscope

(a)  

57.

viruses which infect bacteria

a)

pathogens

b)

bacteriophages

c)

virions

d)

prions

58.

who coined the term "virus" (slimy fluid)

a)

Stanely B. Prusiner

b)

Hans Christian Gram

c)

Martinus Beijerinck

d)

Ben Taylor

59.

virus can be observed through an...

a)

Electron Microscope

b)

Compound Microscope

c)

Simple Microscope

d)

Scanning Electron Microscope

60.

is a protein shell that encloses the nucleic acid.

a)

Nucleoplasm

b)

Plasma Membrane

c)

Capsid

d)

Nucleocapsid

61.

all viruses contain _______ ____.

(a)  

62.

the capsid is composed of many small protein units called...

a)

histones

b)

ribosomes

c)

capsid

d)

capsomere

63.

many types of virus have a (a)   envelope surrounding the nucleocapsid.

64.

are complete virus particles which consist of nucleic acid and an outer shell of protein.

a)

Virions

b)

Virus

c)

Prions

d)

Bacteriophages

65.

microscopic parasitic infectious agent that replicates only inside the living cells of an organism.

a)

Viroids

b)

Prions

c)

Carcinoma

d)

Viruses

66.

viruses originated in the primordial soup and coevolved with bacteria and archaea.

a)

Evolution Theory

b)

Retrograde Evolution Theory

c)

Coevolution Theory

d)

Escaped Gene Theory

67.

viruses are pieces of host cell RNA or DNA that have escaped from living cells, and are no longer under cellular control.

a)

Coevolution Theory

b)

Escaped Gene Theory

c)

Retrograde Evolution Theory

d)

Evolution Theory

68.

cause the lytic cycle which destroys bacterial cell walls

a)

Virulent bacteriophages

b)

Temperate bacteriophages

c)

Bacteriophages

d)

Lysogenic

69.

do not immediately initiate the lytic cycle, but rather,

their DNA remains integrated into the bacterial cell chromosome, generation after generation

a)

Bacteriophages

b)

Temperate bacteriophages

c)

Virulent bacteriophages

d)

Pathogenic Bacteriophages

70.

is any virus that remains in its host organism without undergoing replication.

a)

Lysogenic Bacteriophages

b)

Animal Viruses

c)

Latent Viruses

d)

Oncogenic Viruses

71.

virus which causes cancer

a)

Ongenic Viruses

b)

Malignant Viruses

c)

Ocology Viruses

d)

Oncogenic Viruses

72.

short naked fragments of single stranded RNA. they also regarded as RELICS OF A PRIMITIVE RNA world.

a)

Viroids

b)

Prions

c)

Bacteria

d)

Virus

73.

who coined the term "prion"?

a)

Ben Taylor

b)

Stanely B. Prusiner

c)

Hans Christian Gram

d)

Martinus Beijerinck

74.

prion is a combination of?

(a)  

75.

are small infectious proteins that causes fatal neurologic spongiform diseases in animals or even humans

a)

Animal Viruses

b)

Viroids

c)

Prions

d)

Virus

76.

softens the waxes to allow dye to penetrate the wall

a)

carbol fuschin

b)

mordant

c)

acid alcohol

d)

methylene blue

77.

a type of differential stain which uses acidic and basic dyes to stain background & bacterial cells respectively so that presence of capsule is easily visualized.

a)

flagella stains

b)

capsule stains

c)

endospore stains

d)

india ink method

78.

what is the color of gram positive bacteria?

a)

crystal violet

b)

blue

c)

red or pink

d)

black

79.

what is the color of gram negative bacteria?

a)

Crystal Violet

b)

Blue

c)

Red or Pink

d)

Black

80.

used to determine cell shape, size, and arrangement. It uses only one kind of stain.

a)

Special Staining

b)

Simple Staining

c)

Differential Staining

d)

Structural Staining

81.

usually require more than one stain. These procedures permit

the differentiation of cell types or cell structures.

a)

Special Staining

b)

Structural Staining

c)

Differential Staining

d)

Simple Staining

82.

procedures are done to observe capsules , spores and flagella.

a)

Special Staining (Structural Staining)

b)

Differential Staining

c)

Simple Staining

d)

Compound Staining

83.

These are bacteria which lose their cell walls during adverse growth conditions but revert to their original shape when placed in favorable growth conditions.

(a)  

84.

These are bacteria that lack cell walls thus appear in various shapes (eg.mycoplasma)

a)

Apomorphic

b)

Autamorphic

c)

Pleomorphic

d)

Geomorphic

85.

who developed gram staining?

a)

Hams Christian Gram

b)

Hans Christopher Gram

c)

Han Christian Gram

d)

Hans Christian Gram

86.

____ ________ bacteria are neither consistently blue nor red on gram staining

(a)  

87.

have a high lipid content (mycolic acid) in their cell walls, hence cannot be stained using the gram stain.

(a)  

88.

utilizes heat and phenol (carbolic acid) to help the penetration of the dye, basic fuchsin, to the inside of mycobacterial cells, which are impermeable to basic dyes in routine

stains.

a)

kinyoun acid fast staining procedure

b)

zheil-neelsein stain

c)

ziehl-neelsen stain

d)

zhiel - neelsen stain

89.

does not require heating, aka cold method

(a)  

90.

____ _____ and ___ content of the mycobacterial cell walls is thought to be the reason for such impermeability.

(a)  

91.

spiral shaped bacteria and one half of bacilli are ?

a)

motile

b)

nonmotile

92.

cocci are ?

a)

motile

b)

nonmotile

93.

utilize molecular oxygen for energy

a)

aerobes

b)

obligate aerobes

c)

microaerophiles

d)

aerotolerant anaerobes

94.

require an atmosphere containing 20-21% O2.

a)

Aerobes

b)

Obligate Aerobes

c)

Microaerophiles

d)

Anaerobes

95.

do not require O2 for life and reproduction

(a)  

96.

can only grow in an anaerobic environment.

a)

Anaerobes

b)

Obligate Anaerobes

c)

Aerotolerant Anaerobes

d)

Facultative Anaerobes

97.

require lower concentrations of O2 (5%).

(a)  

98.

grow better in the absence of O2 but can survive in its

presence.

(a)  

99.

can survive in the presence or absence of oxygen.

(a)  

100.

- an established method, often used in diagnostic microscopy, for contrasting a thin specimen with an optically opaque fluid. In this technique, the background is stained, leaving the actual specimen untouched, and thus visible

(a)  

101.

These are “ancient” microorganisms that are able to live in extreme environment.

They possess cell walls without peptidoglycan.

(a)  

102.

diverse collection of obligately intracellular Gram-negative, non-sporeforming highly pleomorphic bacteria found in ticks, lice, fleas, mites, chiggers, and mammals. These bacteria cause arthropod borne diseases such as Rocky Mountain Spotted Fever

a)

Chlamydias

b)

Mycoplasmas

c)

Rickettsia

d)

Archaea

103.

are obligate intracellular pathogens transmitted by inhalation of aerosols or by direct contact between hosts.

They are referred to as energy parasites because they can produce ATP's.

a)

Rickettsia

b)

Chlamydias

c)

Mycoplasmas

d)

Archaea

104.

What is the most common causes of sexually transmitted urethritis?

(a)  

105.

All bacteria are not photosynthetic.

a)

TRUE

b)

FALSE

106.

Smallest of the cellular microbes. They lack cell walls thus are pleomorphic. It causes atypical pneumonia and genitourinary infections.

a)

Rickettsia

b)

Chlamydias

c)

Mycoplasmas

d)

Archaea

107.

Chlamydias produce fried egg shaped colonies when cultured.

a)

TRUE

b)

FALSE

108.

the first person to use the word "cell" to identify microscopic structures when he was describing boxlike cells of the cork which reminded him of the cells of a monastery.

(a)  

109.

Who are the scientists who contributed to the cell theory? (3)

(a)  

110.

known as the "father of pathology".

▪ He introduced the third dictum in cell theory: Omnis cellula e cellula ("All cells come from cells")

(a)  

111.

Who is the father of taxonomy?

(a)  

112.

is the science of classification of living organisms.

(a)  

113.

assignment of names according to international rules.

a)

Nomenclature

b)

Classification

c)

Identification

d)

Description

114.

arrangement of organism into taxonomic groups on the basis of similarities.

(a)  

115.

determining whether an isolate belongs to one of the established, named taxa or represents a previously unidentified species

(a)  

116.

These are external structures that provide rigidity,

shape and protection to the cell and may contain cellulose, pectin, lignin, chitin and some mineral salts.

(a)  

117.

membrane bound structures containing photosynthetic pigments.

(a)  

118.

is made up of filamentous proteins which strengthens, supports, stiffens and gives shape to the cell.

(a)  

119.

are membrane-bound organelles that generate most of the chemical energy needed to power the cell's biochemical reactions.

(a)  

120.

are organelles that carry out oxidation reactions leading to the production of hydrogen peroxide. They also contain the enzyme catalase which decomposes hydrogen peroxide by converting it to water and oxygen.

(a)  

121.

Chemical energy produced by the

mitochondria is stored as

(a)  

122.

a group of flattened disc-like structures located close to the endoplasmic reticulum. It packages proteins into small membrane enclosed vesicles for transport within or

outside the cell.

(a)  

123.

(protein factory) consist mainly of rRNA and proteins. It is also called 80S which consists of two sub-units, a large 60S and a small 40S.

(a)  

124.

forms a series of flattened sacs within the cytoplasm of eukaryotic cells. It is responsible for the synthesis, folding, modification, and transport of proteins .

(a)  

125.

is enclosed by membranes. It contains the cell's DNA and directs the synthesis of proteins and ribosomes.

(a)  

126.

is gel-like, water-based fluid that occupies the majority of the volume of the cell. It is the site of metabolic reactions in the cell.

(a)  

127.

Cytoplasm composed of the cytosol, organelles, and various particles and granules called ?

(a)