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WorksheetsMICROBIO
Total questions: 127
Worksheet time: 4hrs 14mins
Study of organisms too small to be seen by the naked eye.
(a)
Organisms which are too small to be seen by the naked eye
(a)
Two major categories of microbes:
Cellular Microbes
Acellular Microbes
Granular Microbes
Agranular Microbes
(a) (also called infectious particles), includes viruses and prions.
(a) (also called microorganisms), includes all bacteria, all archaea, all protozoa, some algae, and some fungi.
Microbes that cause diseases.
(a)
Microbes that do not cause disease
(a)
Harmless microorganisms consist of the indigenous microflora that lives on or in the body. They cause disease only if they accidentally invade the “wrong place at the right time”.
(a)
Occur when opportunistic microorganisms gain access to a part
of the body where they do not belong.
(a)
What is the importance of microorganisms? (select all that applies)
Microorganisms form the INDIGENOUS MICROFLORA of the human body.
Some microorganisms are OPPORTUNISTIC PATHOGENS.
Some microorganisms are SAPROPHYTES/DECOMPOSERS.
Microorganisms are sources of food.
Bacteria can be manipulated to produce enzymes and proteins they normally would not produce
microflora Inhibit the growth of pathogens by :
(select all that applies)
Occupying space
Depleting food supply
Secreting materials (waste products, toxins, antibiotics ) that prevent growth
of pathogens
Nourishing the organisms and providing them energy.
Organisms that live on dead or decaying organic matter. They aid in fertilization by returning inorganic nutrients to soil. They also break down dead and decaying organic matter into nutrients, phosphates and other chemicals for growth of plants.
(a)
Microorganisms are sources of food.
(select all that applies)
Algae
Plankton
Phytoplankton
Zooplankton
Microplankton
Bacteria synthesize chemicals that our body needs, but cannot synthesize.
true
false
branch of microbiology dealing with plant-associated microbes and plant and animal diseases.
(a)
the manipulation (as through genetic engineering) of living organisms or their
components to produce useful usually commercial products (as pest resistant crops, new bacterial strains, or novel pharmaceuticals)
(a)
Study of composition and physiology of microbial communities in the environment
(a)
branch of medicine concerned with the prevention, diagnosis and treatment of
infectious diseases.
(a)
The insertion of foreign genes into microorganisms to enable the microorganisms to produce specific gene products or to enable them to be used for other purposes.
(a)
Study of the structure and functions of microbial cells.
(a)
The study of ancient microorganisms.
(a)
science based on the detection of risks associated with the production, manufacture and consumption of foods and water
(a)
concerned with microbial (bacterial, fungal, viral) diseases of domesticated
vertebrate animals (livestock, companion animals, fur-bearing animals, game,
poultry, fish) that supply food, other useful products or companionship.
(a)
Known as the "Father of microbiology", "Father of bacteriology" and "Father of protozoology". He was the first to see live bacteria and protozoa which he called animalcules by using single lens or simple microscope to view these microorganisms.
Anton Van Leeuwenhoek
Louis Pasteur
Robert Koch
Edward Jenner
French chemist that;
▪ Disproved the theory of spontaneous generation or abiogenesis
▪ coined the terms aerobes and anaerobes
▪ Discovered Pasteurization
▪ Discovered silkworm disease, various vaccines (rabies, anthrax), process of alcohol
fermentation
▪ Germ theory of disease
Anton Van Leeuwenhoek
Louis Pasteur
Robert Koch
Edward Jenner
German physician that;
▪ Discovered: Anthrax bacillus, M. tuberculosis, V. cholerae
▪ able to obtain pure cultures of bacteria using the petri dish (R.J. Petri) and Agar
as solidifying agent
▪ Tuberculin skin test
Anton Van Leeuwenhoek
Louis Pasteur
Robert Koch
Edward Jenner
contributed to the development of the first vaccine which was against smallpox
Anton Van Leeuwenhoek
Louis Pasteur
Robert Koch
Edward Jenner
discovered the cell when he looked at a sliver of cork through a microscope lens
Robert Hooke
Joseph Lister
Paul Ehrlich
Hans Chritian Gram
pioneer of antiseptic surgery
Robert Hooke
Joseph Lister
Hans Chritian Gram
Hans Chritian Gram
credited with finding the cure (salvarsan) for syphilis
Robert Hooke
Joseph Lister
Paul Ehrlich
Hans Chritian Gram
a Danish bacteriologist noted for his development of the Gram stain
Robert Hooke
Joseph Lister
Paul Ehrlich
Hans Chritian Gram
includes plants and animals that float along at the mercy of the sea's tides and
currents. Their name comes from the Greek meaning "drifter" or "wanderer."
(a)
results when a person ingests a toxin (poisonous substance) that has been produced by a microbe.
(a)
used to express the sizes of microorganisms.
(a)
the basic unit of length in the metric system.
(a)
The size of RBC's, bacteria and protozoa is measured as (a)
The size of viruses is measured as (a) .
A tiny ruler within the eyepiece of a compound light microscope, which is used to measure the sizes of cellular microorganisms.
(a)
an optical instrument that is used to observe tiny objects, that cannot be seen at all with the unaided human eye.
(a)
A (a) contains only one magnifying lens.
A (a) contains more than one magnifying lens. It uses visible light as the source of illumination.
Total magnification of the compound light microscope is calculated by multiplying the magnifying power of the ocular lens by the magnifying power of the objective being used.
true
false
Credited for developing the first compound microscope.
(a)
He used compound microscopes with three lens systems.
(a)
He authored, Micrographia, a 1665 volume documenting experiments he had
made with a microscope. He coined the term "cell" while discussing the structure of cork.
(a)
The capacity of a microscope to distinguish or separate two adjacent
objects or points from one another.
(a)
an instrument that uses visible light and magnifying lenses to examine small objects not visible to the naked eye, or in finer detail than the naked eye allows.
(a)
Microscope where objects are observed against a bright background. Has Maximum magnification: 1000x, Resolution: 0.2um
(a)
Microscope where Illuminated objects are seen against a dark background. Has Resolving power: 0.2000um, Useful magnification: 1000x.
(a)
Microscope that uses the differences in the phase of light transmitted or reflected by a specimen to form
distinct, contrasting images of different parts of the specimen.
(a)
Compound microscope with an ultraviolet radiation source. Often used in immunology laboratories to demonstrate that antibodies stained with a fluorescent dye have combined with specific antigens; this is a type of immunodiagnostic procedure.
(a)
Couples a laser light source to a light microscope. A laser beam is bounced off a mirror that directs the beam through a scanning device. Cells are often stained with fluorescent dyes to make them more visible.
(a)
Uses an electron beam as a source of illumination and magnets to focus the beam. Only for dead organisms.
(a)
Type of Electron Microscope which allows examination of cellular and viral
Ultrastructure. Reveals the finest detailed structure of cells and viruses.
• Resolving power: 0.2nm
• Useful magnification: 200,000x
(a)
Type of Electron Microscope which is designed to create an extremely detailed
3D view of the specimen. Useful for examining surface features of cells and viruses/
• Resolving power: 20nm
• Useful magnification: 10,000x
(a)
Microscope that measures surface features by moving a sharp probe over the object’s surface. It enables scientists to view atoms or molecules on the surface of a specimen.
examples,
• Scanning Tunneling Microscope
• Atomic Force Microscope
(a)
viruses which infect bacteria
pathogens
bacteriophages
virions
prions
who coined the term "virus" (slimy fluid)
Stanely B. Prusiner
Hans Christian Gram
Martinus Beijerinck
Ben Taylor
virus can be observed through an...
Electron Microscope
Compound Microscope
Simple Microscope
Scanning Electron Microscope
is a protein shell that encloses the nucleic acid.
Nucleoplasm
Plasma Membrane
Capsid
Nucleocapsid
all viruses contain _______ ____.
(a)
the capsid is composed of many small protein units called...
histones
ribosomes
capsid
capsomere
many types of virus have a (a) envelope surrounding the nucleocapsid.
are complete virus particles which consist of nucleic acid and an outer shell of protein.
Virions
Virus
Prions
Bacteriophages
microscopic parasitic infectious agent that replicates only inside the living cells of an organism.
Viroids
Prions
Carcinoma
Viruses
viruses originated in the primordial soup and coevolved with bacteria and archaea.
Evolution Theory
Retrograde Evolution Theory
Coevolution Theory
Escaped Gene Theory
viruses are pieces of host cell RNA or DNA that have escaped from living cells, and are no longer under cellular control.
Coevolution Theory
Escaped Gene Theory
Retrograde Evolution Theory
Evolution Theory
cause the lytic cycle which destroys bacterial cell walls
Virulent bacteriophages
Temperate bacteriophages
Bacteriophages
Lysogenic
do not immediately initiate the lytic cycle, but rather,
their DNA remains integrated into the bacterial cell chromosome, generation after generation
Bacteriophages
Temperate bacteriophages
Virulent bacteriophages
Pathogenic Bacteriophages
is any virus that remains in its host organism without undergoing replication.
Lysogenic Bacteriophages
Animal Viruses
Latent Viruses
Oncogenic Viruses
virus which causes cancer
Ongenic Viruses
Malignant Viruses
Ocology Viruses
Oncogenic Viruses
short naked fragments of single stranded RNA. they also regarded as RELICS OF A PRIMITIVE RNA world.
Viroids
Prions
Bacteria
Virus
who coined the term "prion"?
Ben Taylor
Stanely B. Prusiner
Hans Christian Gram
Martinus Beijerinck
prion is a combination of?
(a)
are small infectious proteins that causes fatal neurologic spongiform diseases in animals or even humans
Animal Viruses
Viroids
Prions
Virus
softens the waxes to allow dye to penetrate the wall
carbol fuschin
mordant
acid alcohol
methylene blue
a type of differential stain which uses acidic and basic dyes to stain background & bacterial cells respectively so that presence of capsule is easily visualized.
flagella stains
capsule stains
endospore stains
india ink method
what is the color of gram positive bacteria?
crystal violet
blue
red or pink
black
what is the color of gram negative bacteria?
Crystal Violet
Blue
Red or Pink
Black
used to determine cell shape, size, and arrangement. It uses only one kind of stain.
Special Staining
Simple Staining
Differential Staining
Structural Staining
usually require more than one stain. These procedures permit
the differentiation of cell types or cell structures.
Special Staining
Structural Staining
Differential Staining
Simple Staining
procedures are done to observe capsules , spores and flagella.
Special Staining (Structural Staining)
Differential Staining
Simple Staining
Compound Staining
These are bacteria which lose their cell walls during adverse growth conditions but revert to their original shape when placed in favorable growth conditions.
(a)
These are bacteria that lack cell walls thus appear in various shapes (eg.mycoplasma)
Apomorphic
Autamorphic
Pleomorphic
Geomorphic
who developed gram staining?
Hams Christian Gram
Hans Christopher Gram
Han Christian Gram
Hans Christian Gram
____ ________ bacteria are neither consistently blue nor red on gram staining
(a)
have a high lipid content (mycolic acid) in their cell walls, hence cannot be stained using the gram stain.
(a)
utilizes heat and phenol (carbolic acid) to help the penetration of the dye, basic fuchsin, to the inside of mycobacterial cells, which are impermeable to basic dyes in routine
stains.
kinyoun acid fast staining procedure
zheil-neelsein stain
ziehl-neelsen stain
zhiel - neelsen stain
does not require heating, aka cold method
(a)
____ _____ and ___ content of the mycobacterial cell walls is thought to be the reason for such impermeability.
(a)
spiral shaped bacteria and one half of bacilli are ?
motile
nonmotile
cocci are ?
motile
nonmotile
utilize molecular oxygen for energy
aerobes
obligate aerobes
microaerophiles
aerotolerant anaerobes
require an atmosphere containing 20-21% O2.
Aerobes
Obligate Aerobes
Microaerophiles
Anaerobes
do not require O2 for life and reproduction
(a)
can only grow in an anaerobic environment.
Anaerobes
Obligate Anaerobes
Aerotolerant Anaerobes
Facultative Anaerobes
require lower concentrations of O2 (5%).
(a)
grow better in the absence of O2 but can survive in its
presence.
(a)
can survive in the presence or absence of oxygen.
(a)
- an established method, often used in diagnostic microscopy, for contrasting a thin specimen with an optically opaque fluid. In this technique, the background is stained, leaving the actual specimen untouched, and thus visible
(a)
These are “ancient” microorganisms that are able to live in extreme environment.
They possess cell walls without peptidoglycan.
(a)
diverse collection of obligately intracellular Gram-negative, non-sporeforming highly pleomorphic bacteria found in ticks, lice, fleas, mites, chiggers, and mammals. These bacteria cause arthropod borne diseases such as Rocky Mountain Spotted Fever
Chlamydias
Mycoplasmas
Rickettsia
Archaea
are obligate intracellular pathogens transmitted by inhalation of aerosols or by direct contact between hosts.
They are referred to as energy parasites because they can produce ATP's.
Rickettsia
Chlamydias
Mycoplasmas
Archaea
What is the most common causes of sexually transmitted urethritis?
(a)
All bacteria are not photosynthetic.
TRUE
FALSE
Smallest of the cellular microbes. They lack cell walls thus are pleomorphic. It causes atypical pneumonia and genitourinary infections.
Rickettsia
Chlamydias
Mycoplasmas
Archaea
Chlamydias produce fried egg shaped colonies when cultured.
TRUE
FALSE
the first person to use the word "cell" to identify microscopic structures when he was describing boxlike cells of the cork which reminded him of the cells of a monastery.
(a)
Who are the scientists who contributed to the cell theory? (3)
(a)
known as the "father of pathology".
▪ He introduced the third dictum in cell theory: Omnis cellula e cellula ("All cells come from cells")
(a)
Who is the father of taxonomy?
(a)
is the science of classification of living organisms.
(a)
assignment of names according to international rules.
Nomenclature
Classification
Identification
Description
arrangement of organism into taxonomic groups on the basis of similarities.
(a)
determining whether an isolate belongs to one of the established, named taxa or represents a previously unidentified species
(a)
These are external structures that provide rigidity,
shape and protection to the cell and may contain cellulose, pectin, lignin, chitin and some mineral salts.
(a)
membrane bound structures containing photosynthetic pigments.
(a)
is made up of filamentous proteins which strengthens, supports, stiffens and gives shape to the cell.
(a)
are membrane-bound organelles that generate most of the chemical energy needed to power the cell's biochemical reactions.
(a)
are organelles that carry out oxidation reactions leading to the production of hydrogen peroxide. They also contain the enzyme catalase which decomposes hydrogen peroxide by converting it to water and oxygen.
(a)
Chemical energy produced by the
mitochondria is stored as
(a)
a group of flattened disc-like structures located close to the endoplasmic reticulum. It packages proteins into small membrane enclosed vesicles for transport within or
outside the cell.
(a)
(protein factory) consist mainly of rRNA and proteins. It is also called 80S which consists of two sub-units, a large 60S and a small 40S.
(a)
forms a series of flattened sacs within the cytoplasm of eukaryotic cells. It is responsible for the synthesis, folding, modification, and transport of proteins .
(a)
is enclosed by membranes. It contains the cell's DNA and directs the synthesis of proteins and ribosomes.
(a)
is gel-like, water-based fluid that occupies the majority of the volume of the cell. It is the site of metabolic reactions in the cell.
(a)
Cytoplasm composed of the cytosol, organelles, and various particles and granules called ?
(a)
