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WorksheetsBAC-T-2
Total questions: 67
Worksheet time: 52mins
Preferred manner of collection for urine
(a)
to confirm that the specimen is sputum, we use ____. What is the (+) result ?
Barlett's classification: Less than 10 Squamos Epithelial Cells and more than 25 PMNs
Oxalic determination: Less than 10 Squamos Epithelial Cells and more than 25 PMNs
Barlett's classification: more than 10 Squamos Epithelial Cells and more than 25 PMNs
None
For non sterile sputum collection ,what are the gold standard digesting and decontaminating agent?
Zephiran- Trisodium Phosphate; 5% oxalic acid
N- acetyl-l-cysteine; NaOH
N- acetyl-l-cysteine; Oxalic acid
Z-TSP; NaOH
The most common cause of UTI in young females
(a)
specimen to collect if the patient is intubated /on a ventilator
(a)
Which of the following is UNTRUE
Dilution factor - 1000: 1ul loop
Dilution factor - 100: 10 ul loop
Colony count: <100, 000
formula = colonies counted x dilution factor
Used to support the growth of fastidious organisms
Enriched media
Enrichment media
Selective media
Biphasic media
Select the selective differential media
Mac
EMB
TCBS
ONPG
MHA
Acts as an inhibitor in CTBA
potassium citrate
potassium oxalate
potassium tellurite
potassium cyanide
selective media for mycobacterium
(a)
HEPA filter filtrates the air by removing objects larger than 0.3 um. HEPA means
(a)
Class III cabinet uses what system
open
close
open and close
What are the descriptions of each of the following (arranged from BSL1-BSL4)
minimal risk; thru ingestion; thru inhalation; life threatening
thru ingestion; thru inhalation; minimal risk; life threatening
life threatening; inhalation; ingestion, minimal risk
complete sestruction and removal of all bacterial organisms including SPORES.
Sterilization
Disinfection
Both
Carried out at 121 degC 30 mins at 15 psi
sterilize used and unused media
or contaminated microbiological materials
infectious medical waste
Geobacillus stearrothermophilus is a biological indicator of autoclaving
TRUE
FALSE
This is not killed by autoclaving.
(a)
What is the method of killing prions
70% iodophor
15% sodium chloride
extended steam sterilization with 1M iodophor
extended steam sterilization with 1M sodium hypochlorite
Bacillus subtilis var niger -______
_______- indicator for ionizing gamma rays
Bacillus subtilis var. globijii -
for cold sterilization
Bacillus pumilus
biological indicator of oven
biological indicator of oven
Bacillus pumilus
for cold sterilization
for hot bath
Bacillus pumilus
biological indicator of ove
Carried out at 100 degC 30 mins 3 days
(a)
- Carried out at (a) 2 hrs for 3 days
Inspissation is carried out in medias like Lowenstein Jensen, Petragnani's media, ATS that contains _____
egg
gamma rays
blood
glycogen
Correlate:
_____: ionization: gamma rays
_____: uv sterilization
sterilization; inspissation
inspissation; sterilization
inspissation; disinfection
sterilization; disinfection
disinfection; sterilization
disinfection by removing pathogens in food
(a)
best antiseptic
iodophor
chlorine
alcohol
bleach
difference of antiseptic and disinfectant
A: skin
D: surfaces
A: surfaces
D: skin
both
standard disinfectant
(a)
10% HOUSEHOLD BLEACH SODIUM HYPOCHLORITE is best for removing blood spills, however, _____ can be used as a substitute
vinegar
chlorine
alcohol
iodine
dilution of bleach in porous surfaces and for hard and smooth surfaces(no spaces)
(a)
burning to dispose infectious materials
cremation
incineration
both
Most effective way to stop the chain of infection
(a)
arrangement: DONNING
(a)
DOFFING arrangement
Gloves Goggles
Mask
Gown
Handwash
Gloves
Mask
Gown
Goggles
Handwashing
Gloves
Gown
Goggles
Mask
Handwash
Gloves
Goggles
Gown
Mask
Handwashing
QC for autoclave spore testing
(a)
What is the most critical step in Gram staining
Staining
Counterstaining
Applying mordant
Decolorization
overdecolorization can lead to
false gram positive result
false gram negative result
both
Hucker's modification for fungi uses vias but the crystal violet is added with
(a)
Size of AFB smear
(a)
preferred method is Ziehl Neelsn
AF organisms
DSSM
both
Specify the drcolorizer used in acid fast staining
(a)
select the antibiotic that prevents bacterial growth
Tetracycline,
Chloramphenicol
streptomycin
erythromycin
Vancomycin
Q.V.M.A.P. is the mnemonics for bactericidal antibiotics
(a)
qualitative method of AST
(a)
Kirby Bauer Disk uses what media
(a)
Macfarland solution is composed of
sulfuric acid and barium chloride
sodium chloride and barium sulfate
sulfuric acid and barium sulfate
unused antibiotics are stored in what temperature
(a)
-20 degC in a (a)
in AST , the sterile cotton swab is straked in what method
zigzag pattern
not overlapping
overlapping
waiting time before applying the disk
(a)
Plate size:
____: no more than 5 antibiotics
150 mm: _____antibiotics
50 mm; 12
100 mm; 10
100 mm; 12
50 mm; 15
In sxt, what is measured if there are 2 concentric zone
(a)
the zone of inhibition is measure by using a ruler or a
(a)
To light inoculum, dry agar , thin agar will result to FALSE SUSCEPTIBLE
To heavy inoculum, too much ,moisture, thick agar : FALSE RESISTANT
(a)
In E- test(susceptibility test), the + result is
(a)
Use of Cefinase disk to impregnate and beta lactams Substrate penicillinase resistant (+) result: pink to red
D test
Beta lactamase
cephalosporinase test
E test
(+) is rose or mauve colonies
mac agar
d test
oxacillin agar
chromogenic agar
Oxacillin Screen Agar uses 6ug oxacillin with
10% NaCl
2% NaCl
4% NaCl
none
D-test is used for strains of (a)
If incase in AST the eryth and clin have different result or discrepant, then D-test is performed
(a)
what antibiotics are placed in the media for D-test
clindamycin and erythromycin
erythromycin and vancomycin
penicillin and clindomycin
none
(+) D-test( wit hzone of inhibition)- we report it as Clindamycin (a)
AST used for S. pneumonia is
(a)
in penicillin resistance , it is resistant if zone of inhibition if the result is
(a)
what makes beta lactam antibiotics ineffective
(a)
positive result of modified hodge test
(a)
automation program of bacterial identification and susceptibility
(a)
faster than vitek
(a)
