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POP QUIZ DNA REPLICATION , TRANSCRIPTION & TRANSLATION

Total questions: 50

Worksheet time: 53mins

Name
Class
Date
1.
Which do DNA and RNA have in common?
a)
Both are double stranded.
b)
Both contain ribose molecules.
c)
Both contain phosphate groups.
d)
Both contain uracil.
2.

The backbone of polynucleotide is made up of

a)

amino acids and sugars

b)

nucleotide bases, including thymine

c)

nucleotide bases, including uracil

d)

sugar-phosphate molecules

3.

What is the complimentary mRNA sequence to the DNA sequence

5' A-T-T-G-C-A 3'

a)

3' T-A-A-C-G-T 5'

b)

5' U-A-A-C-G-T 3'

c)

3' U-A-A-C-G-U 5'

d)

5' T-A-A-G-C-U 3'

4.

Which defines a codon on mRNA?

a)
pair of nucleic acid and sugar
b)
pair of phosphate and sugar
c)
three-base code
d)
two-base code
5.
The "central dogma" states that genetic information flows from 
a)
Protein-->mRNA-->DNA
b)
Protein-->DNA-->mRNA
c)
mRNA-->DNA-->Protein
d)
DNA-->mRNA-->Protein
6.
Transcription takes place in the 
a)
cytoplasm
b)
ribosome
c)
mitochondrion
d)
nucleus
7.
The anticodon of a particular tRNA molecule is 
a)
complementary to the corresponding mRNA codon.
b)
complementary to the corresponding triplet in rRNA.
c)
the part of tRNA that bonds with a specific amino acid.
d)
catalytic, making the tRNA a ribozyme.
8.

The region on mRNA that non as non-coding region

a)

introns

b)

exons

c)

codons

d)

anticodons

9.
Translation occurs:
a)
Outside of the Nucleus
b)
Inside of the Nucleus
10.
Translation is crucial to the process of making proteins. Which statement best describes what takes place during translation? 
a)
A copy of chromosomal DNA is created 
b)
Information in mRNA is converted into a sequence of amino acids in a protein 
c)
A RNA copy of a DNA strand is made.
d)
Instructions from DNA in the nucleus are brought to the cytoplasm 
11.
Smaller segments of RNA nucleotides that transport amino acids to the ribosomes.
a)
mRNA
b)
tRNA
c)
rRNA
d)
DNA
12.

Which enzyme joins the Okazaki fragments in the lagging strand?

a)

DNA Helicase

b)

DNA Polymerase

c)

DNA Ligase

d)

Primase

13.

This picture is illustrating the ____ nature of DNA.

a)

antiparallel

b)

semiconservative

c)

helical

d)

radioactive

14.

How do the leading and the lagging strands differ?

a)

The leading strand is synthesized in the same direction as the movement of the replication fork, and the lagging strand is synthesized in the opposite direction.

b)

The leading strand is synthesized at twice the rate of the lagging strand.

c)

The leading strand is synthesized in short fragments that are ultimately stitched together, whereas the lagging strand is synthesized continuously.

d)

The leading strand is synthesized by adding nucleotides to the 3' end of the growing strand, and the lagging strand is synthesized by adding nucleotides to the 5? end.

15.

Which enzyme is responsible for adding the RNA primer to the template strand?

a)

Helicase

b)

DNA Polymerase

c)

Ligase

d)

Primase

16.

What is the result of transcription?

a)

The synthesis of RNA from protein

b)

The synthesis of protein from RNA

c)

The synthesis of protein from DNA

d)

The synthesis of RNA from DNA

17.

What is the result of translation?

a)

The synthesis of RNA from protein

b)

The synthesis of protein from RNA

c)

The synthesis of protein from DNA

d)

The synthesis of RNA from DNA

18.

Which organelle does required in translation?

a)

Nucleus

b)

Ribosome

c)

Mitochondria

d)

Golgi apparatus

19.

What is the role of tRNA?

a)

To treat the DNA strand

b)

To transfer codons

c)

To transfer amino acids

d)

To tackle mutations

20.

What is the start codon?

a)

UAC

b)

TAC

c)

AUG

d)

GUA

21.

How many codons are required for one amino acid?

a)

1

b)

2

c)

3

d)

4

22.

How many codons are required for three amino acids?

a)

1

b)

2

c)

3

d)

4

23.

Which of the following is the major enzyme that involved in DNA

replication?

I. Helicase

II. Reverse transcriptase

III. Topoisomerase

IV. DNA polymerase

V. Aminoacyl-tRNA synthetase

a)

I, III and IV

b)

I, III and V

c)

I,II, III and IV

d)

II, III, IV and V

24.

The following events occur in the replication of DNA.


1. Bonds between complementary bases break

2. Bonds between complementary bases form

3. DNA molecule uncoils

4. Opposite strands separate

5. Sugar-phosphate backbone form

6. Free nucleotides align with complementary nucleotides on each strand


In which order do these events take place?

a)

1 3 6 4 2 5

b)

3 1 4 6 2 5

c)

3 6 1 4 5 2

d)

4 3 1 6 5 2

25.

What is the role of DNA ligase in DNA replication?

a)

Synthesize of short RNA primer by adding RNA nucleotide

b)

Replaces the RNA primer by adding the DNA nucleotide

c)

Join Okazaki fragment at lagging strand by forming phosphodiester bond

d)

Unwind the double stranded DNA to become single stranded DNA

26.

In DNA

replication, new DNA nucleotides are added to a growing DNA by…………………

a)

DNA helicase

b)

DNA polymerase

c)

RNA polymerase

d)

RNA primer

27.
Replicate the following strand of DNA: 
AATCATGGA
a)
AATCATGGA
b)
TTAGTACCT
c)
UUAGUACCU
d)
GGATAUCUA
28.
The enzyme that unzips the DNA to prepare for replication
a)
helicase
b)
replicase
c)
polymerase
d)
synthase
29.
The process of DNA replication is called semi conservative because it results in
a)
none of these
b)
Two DNA molecules.  One original molecule and one with two new strands
c)
two exact copies with new strands
d)
two identical molecules, each with 1 original strand and one new.
30.

What enzyme does transcription use?

a)

DNA polymerase

b)

ligase

c)

RNA polymerase

d)

helicase

31.

Which RNA forms part of the ribosomes?

a)

tRNA

b)

mRNA

c)

rRNA

32.
What is occurring in this picture?
a)
Translation
b)
Replication
c)
Translocation
d)
Transcription
33.

Transcribe the following DNA strand: CTG AAT

a)

GAC TTA

b)

CTG AAT

c)

GAC UUA

d)

CUG AAU

34.
How many amino acids are coded for by this sequence of nucleotides:
ATG GGA ACT CCA
a)
4
b)
2
c)
6
d)
12
35.
What would be the tRNA anticodon for the mRNA codon CAG?
a)
GTC
b)
GUC
c)
CUG
d)
CAG
36.
What would happen if we did not have start codons?
a)
Polypeptide chains would continue to grow
b)
Amino acids would bind to tRNA
c)
Protein synthesis wouldn't occur
d)
mRNA would form a double strand
37.
Which of the following occurs first in translation?
a)
The small ribosomal subunit binds to mRNA
b)
The large ribosomal subunit binds to mRNA
c)
tRNA binds to the mRNA
d)
A peptide bond forms between amino acids
38.
What is the correct amino acid sequence for the mRNA code AUGCCAGUAUGA
a)
Met-Pro-Ala-Val
b)
Met-Pro-Val
c)
Tyr-Gly-His
d)
Tyr-Gly-Arg-His
39.

Match the mRNA codon UUA to the correct tRNA anti-codon

a)

AAT

b)

AAU

c)

AUA

d)

ATA

40.

Why must transcription occur in the nucleus?

a)

because DNA can't leave the nucleus

b)

because ribosomes are in the nucleus

c)

because DNA polymerase is found there

d)

because helicase unzips the DNA

41.

Choose the sequence of the mRNA strand that would match up with to the following DNA strand:

GTACAT

a)

CATGTA

b)

GAUCUA

c)

CAUGUA

d)

CUTGTU

42.

Which anticodon would be placed in spot #2

a)

GGC

b)

CCA

c)

GGA

d)

GGT

43.

Which process does this diagram illustrate?

a)

transcription

b)

DNA replication

c)

translation

44.

For initiation of translation, initiator tRNA binds to

a)

hnRNA

b)

AUG codon

c)

UTRs

d)

Promoter

45.

Which of the following is not a terminator codon?

a)

UAA

b)

UGA

c)

UAG

d)

UGU

46.

What is translation?

a)

the process in which DNA is copied to make another molecule of DNA

b)

the process in which DNA is coded into mRNA

c)

the process in which mRNA is decoded into a protein

d)

the process in which enzymes break down mRNA

47.

Use the mRNA below to select the correct amino acid sequence:

AUG-UCA-AAA-UAG

a)

MET-SER-LYS-STOP

b)

MET-THR-LYS-TYR

c)

LEU-SER-CYS-STOP

d)

ILE-LYS-SER-STOP

48.

Which enzyme shows where to begin DNA replication?

a)

Helicase

b)

DNA polymerase

c)

Ligase

d)

Primase

49.

Which DNA strand is replicated continuously?

a)

Leading Strand

b)

Lagging Strand

50.

Which strand produces Okazaki fragments?

a)

Leading Strand

b)

Lagging Strand

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