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DNA to Identify Pathogens

Total questions: 44

Worksheet time: 38mins

Name
Class
Date
1.

Which pathogen is non-living?

a)

Virus

b)

Bacteria

c)

Fungi

d)

Parasite/Protist

e)

They are all alive

2.

Which type of pathogen is responsible for causing the flu, chicken pox, and yellow fever?

a)

viruses

b)

parasites

c)

fungi

d)

bacteria

3.

Which pathogen is treated with antibiotics?

a)

virus

b)

bacteria

c)

parasite

d)

fungi

4.

Athlete's Foot is caused by:

a)

fungus

b)

bacteria

c)

parasite

d)

virus

5.
Which best distinguishes an infectious disease from a noninfectious disease?
a)
Infectious diseases can be cured with antibiotics; noninfectious diseases cannot.
b)
Infectious diseases can be prevented with vaccines; noninfectious diseases cannot.
c)
Infectious diseases can be spread from one organism to another; noninfectious diseases cannot.
d)
Infectious diseases cannot be transmitted from one organism to another; noninfectious diseases can.
6.

The picture below shows an enzyme unwinding and unzipping DNA.

​

​What is the name of this enzyme?

a)

DNA Helicase

b)

DNA Ligase

c)

DNA polyermase

d)

RNA Polymerase

7.

Which of the following DNA nucleotides are paired with the correct corresponding DNA nucleotide? (mark all that apply)

a)

Adenine with Thymine

b)

Guanine with Cytosine

c)

Thymine with Cytosine

d)

Adenine with Uracil.

e)

Uracil with Thymine

8.

Which is the role of DNA polymerase?

a)

Unwinds the original DNA strand

b)

Lays down RNA primer for new nucleotide bases.

c)

Builds new strands by bringing in new nucleotide bases.

d)

Remove Okazaki fragments for new nucleotides.

9.
Nitrogenous bases are joined by which type of bond?
a)
polar bonds
b)
ionic bonds
c)
covalent bonds
d)
hydrogen bonds
10.
Why does DNA replicate?
a)
To create another copy for the new cell
b)
To create a duplicate copy in case the original gets damaged
c)
To create a new copy to replace the damaged original
11.
The process of DNA replication is called semi conservative because it results in
a)
none of these
b)
Two DNA molecules.  One original molecule and one with two new strands
c)
two exact copies with new strands
d)
two identical molecules, each with 1 original strand and one new.
12.

What makes up the SIDES of a DNA ladder?

a)

nitrogen bases

b)

sugars & phosphates

c)

genes & chromosomes

d)

RNA

13.

If one strand of a DNA molecule has the following order of bases, what will be the correct order of bases on the complementary (matching) strand?

AATTGGCC

a)

AATTGGCC

b)

TTAACCGG

c)

GGCCAATT

d)

GACTGACT

14.

What is DNA made up of?

a)

nucleotides

b)

cytoplasm

c)

RNA

d)

cells

15.

The picture below shows the steps of DNA replication beginning with step one and ending with step three.


​Because you are reusing the old, or parent, DNA strand DNA replication is said to be

a)

Semi- Conservative

b)

Semi-discontinuous

c)

Continuous

d)

Fully-Conserveative

16.

Which enzyme is responsible for adding the free nucleotides to the replicating DNA? ​

a)

DNA Helicase

b)

DNA Ligase

c)

DNA polyermase

d)

RNA Polymerase

17.

DNA replication creates two strands of DNA from one. If pink is parental DNA and blue is new DNA - which picture demonstrates the accepted model of DNA replication after the SECOND replication cycle?

a)
b)
c)
d)
18.

Many enzymes are involved in DNA replication. Which is the correct order of enzymes involved in DNA replication from beginning to end?

a)

Helicase, DNA primase, DNA polymerase, Ligase

b)

RNA primase, Helicase, DNA polymerase, Ligase

c)

Helicase, RNA primase, DNA polymerase, Ligase

d)

DNA primase, Helicase, RNA polymerase, Ligase

19.

Which is the last step of DNA replication?

a)

RNA Helicase unwinds original DNA strand

b)

DNA Primase lays down RNA primer for new nucleotide bases.

c)

RNA polymerase builds new strands by bring in new nucleotide bases.

d)

Ligase knits together fragments on lagging strand

20.
Who's work concluded that the %A=%T  and %G=%C
a)
Chargaff
b)
Meischer
c)
Levene
d)
Hammerling
21.

What are the components of a DNA nucleotide? (Mark all that apply)

a)

Deoxyribose

b)

Ligase

c)

Nitrogen Base

d)

Phosphate group

e)

Ribose

22.

What part of the cell cycle is considered interphase? Select all that apply.

a)

G1

b)

G2

c)

S

d)

M

23.

You have DNA and you wish to find other DNA sequences that look like it. Which of the five Basic Blast programs should you use?

a)

Blastn

b)

Blastp

c)

blastx

d)

blastd

24.

What does BLAST stand for?

(a)  

25.

What is the purpose of Polymerase Chain Reaction (PCR) in the process of sequencing DNA?

a)

To separate DNA fragments by size

b)

To synthesize copies of template DNA

c)

To change the structure of template DNA

d)

To terminate the elongation of a DNA strand

26.

Write the correct sequence that matches this chromatogram.

(a)  

27.

What type of sequencing was used to obtain Sue's DNA sequence?

a)

Sanger

b)

Cycle

c)

BLAST

d)

Chargoff

28.

All the genetic material in an organism


is the definition of which key term?

a)

Proteome

b)

Genome

c)

Genotype

d)

Exon

29.

Bioinformatics is related with

a)

Computers

b)

Biology

c)

Both computers and biology

d)

none of these

30.

4. In the nucleus, DNA is first ____ into an RNA molecule.

a)

a. Translated

b)

b. Transcribed

c)

c. Transferred

d)

d. Transmitted

31.

8. Which of the following statements about DNA and RNA is incorrect?

a)

a. Both have cytosine as a base

b)

b. Both are composed of a nitrogenous base

c)

c. The DNA copies specific instructions from the RNA

d)

d. DNA is a double-stranded helix, while RNA is a single-stranded helix

32.
What is the function of a primer?
a)
To identify the particular region of DNA to be copied by PCR.
b)
To copy DNA.
c)
To create DNA nucleotides.
d)
To maintain the temperature of the PCR reaction.
33.

Which column details the probability that the BLAST results were by chance?

4 lines
34.

Which columns when interpreted shows how the DNA query in question aligns with the pathogen DNA?

(a)  

35.
What is DNA sequencing?
a)
The total of genes carried by an individual or cell
b)
Coding genes in order to identify single nucleotide polymorphisms
c)
Deciphering the functions of various parts of the human genome
d)
The process of identifying the order of the bases in DNA
36.
The main goal of the Human Genome Project (HGP) was to:
a)
Identify the unique parts of each person's DNA.
b)
Identify genes responsible for human diseases.
c)
Identify genes responsible for human behavior.
d)
Determine the entire sequence of human DNA.
37.

If a DNA strand contains 60 Cytosine's and 40 cytosine's. What does this mean?

4 lines
38.

What is it called when the unique 3-D shape of a protein has been disrupted or 'unravelled'?

a)

Denaturation

b)

Deproteination

c)

Decoupling

d)

Depeptidasing

39.
What bacterial shape is present in the image?
a)
Bacillus
b)
Coccus
c)
Spirillum
d)
Vibrio
40.

What are the three most common bacteria shape names?

a)

coccus, bacillus, sprillum

b)

round, short, circuluar

c)

bacillus, streptobacillus, staphylococcus

d)

cocci, sarcina, bacillus

41.

What does staphylo mean?

a)

In a line

b)

group of 4

c)

groups or clusters

d)

single

42.

What does strepto mean?

a)

in a line

b)

group of 4

c)

groups or clusters

d)

single

43.

What is Step 3 of DNA sequencing?

1. isolate DNA

2. Separate DNA from pathogen DNA.

3 (a)  

4. Use Sanger Sequencing

44.

BONUS:

Define Meningitis

List 3 consequences of meningitis

4 lines

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