WorksheetsGenetics Exam III Review (Translation Chp. 13)
Total questions: 16
Worksheet time: 10mins
Three sites of ribosomal subunits are called:
(a)
What is the Shine-Dalgarno sequence?
(a)
In eukaryotes, translation occurs in (a)
T or F: The Kozak sequence is considered to increase the efficiency of translation initiation in eukaryotes
True
False
Advantages of closed loop translation in eukaryotes:
Cell saves energy by not translating partially degraded mRNA
Translation occurs at a faster rate in eukaryotes than in prokaryotes due to closed loop
Closed loop structure allows for efficient ribosome cycling
Leads to less mutation than open looped translation
T or F: Bacterial Proteins are homologous to eukaryotic elongation factors (eEFs) and eukaryotic release factors (eRFs).
True
False
Role of IF1
Binds to the initiator fMet-tRNA and transfers it to the P site of the 30s-mRNA complex
Binds to 30s subunit, preventing it from associating with the 50s subunit prematurely
Binds to 30S subunit and prevents aminoacyl tRNA from binding to the A site prematurely
Role of IF2
Binds to initiator fMet-tRNA and transfers it to the P-site of the 30S-mRNA complex
Binds to 30S subunit and prevents aminoacyl tRNA from binding to the A site prematurely
Binds to 30s subunit, preventing it from associating with the 50s subunit prematurely
Role of IF3:
Binds to 30S subunit, preventing it from associating with the 50S subunit prematurely
Binds to 30S subunit and prevents aminoacyl tRNA from binding to the A site prematurely
Binds to the initiator fMet-tRNA and transfers it to the P site of the 30S-mRNA complex
Binds GTP; brings aminoacyl tRNA to the A site of the ribosome
EF-Ts
EF-Tu
EF-G
None of these
Regulates EF-Tu Activity
EF-Ts
EF-Tu
EF-G
None of these
Stimulates translocation; GTP-independent
EF-Ts
EF-Tu
EF-G
None of these
Catalyzes release of polypeptide chain from tRNA and dissociation of the translocation complex; specific for UAA and UAG termination codons
RF1
RF2
RF3
None of these
Behaves like RF1; specific for UGA and UAA codons
RF1
RF2
RF3
None of these
Stimulates RF1 and RF2 release
RF1
RF2
RF3
None of these
The name of the sequence where eukaryotic mRNAs contain a conserved sequence upstream from AUG initiator codon
(a)
