paper chromatography is an example of

type of buffer used when the

- pH level is around 8.0 pH

- for nucleic acid separation

- has high resolution

- has high buffering capacity

In an experiment, Seth used an ion-selective electrode with no dilution of sample. What type of ISE electrode is used?

a pH electrode contained within a plastic jacket with sodium buffer and permeable membrane

under potentiometry, it is an electrode that produces constant potential

I. Anode is positively charged

II. Anion is positively charged

To produce Cellulose acetate, Cellulose is treated with ___

what colligative properties have inversely proportional effect to osmolality

Voltammetry is based on the voltage-potential relationship in an electrochemical cell when the potential is applied. Is the underlined word true?

It is has a measurement >35 fL

An ampholyte has an isoelectric point of 10. Your buffer has pH of 3. How do you describe the pH of the buffer? It is positive or negatively charged?

two supporting mediums that are commonly used in electrophoresis

Components of Electrophoresis:

functions to provide ions that could carry current in the supporting medium

these analytes increases in the morning, except:

Prolonged torniquet application will lead to

prolonged standing (> 30 minutes) increases

high protein and low carbohydrate diet will increase

48 hrs of fasting increases

Caffeine lowers ___ and increases ___

What are the primary contributing factors of Ergonomic Hazards?

Sample A has -3 charge; Sample B has -5 charge

Who will migrate farther?

fastings under lipid profile

Compressed gasses are mixture of gasses under the pressure of

What type of radiation is more harmful to the human body?

What is the flash point according to this NFPA symbol?

Fires involving high temperature cooking oils, what type of fire extinguisher should be used?

common irritant in the cc lab

Stated below are the employer's responsibilities, except:

Clinical and Laboratory Standards Institute is previously known as national Community for Clinical Laboratory Standards (NCCLS)

In what biohazard bag will you throw plastic bottles?

In what biohazard bag will you throw sharps?

It has HEPA filter and used when dealing with potentially infectious elements

In what biohazard bag will you throw the wet wipes used in cleaning the lab station?

wearing gloves, lab gown and face mask inside the laboratory

Fires involving electrical equipment, what type of fire extinguisher should be used?

What is the specific hazard according to this NFPA symbol?

Materials which cause harm by irritating the eyes and/or skin, and cause allergic and/or organ damage

types of luminescence

from higher orbital to lower orbital level

delayed fluorescence

the excitation of the substance does not involve electromagnetic radiation and no monochromators needed, instead the excitation energy comes from a chemical reaction

measures the emitted fluorescent light

basic component of fluorometry:

an atom or molecule that fluoresces

I. Photomultiplier is commonly used due to it being inexpensive

II. Photomultiplier is commonly used due to its sensitivity

I. Emission Monochromator selects the wavelength that is best absorbed by the solution

II. It is also positioned in a straight line from cuvette to photodetector

what light is measured in fluorometry?

a change in pH affects the electron availability of fluorescence which result to quenching

measures the amount of light blocked in a particulate suspension

In Nephelometry the light is measured at 90º degrees, whereas In Turbidimetry the light is measured in a straight line

In Rayleigh, wavelength of light is __ than particle size

In Rayleigh Debye, wavelength of light is __ than particle size

Basic component of chromatography:

● solvent is moving through the column

● can be gas or liquid

● carries the sample

Basic component of chromatography:

● the process of washing out a compound through a column using a suitable solvent

Basic component of chromatography:

● fluid that enters the column and moves the analyte

Chromatographic techniques according to their mobile phase include:

Uses lower temperature for separation, achieving better separation of thermolabile compounds

a separation technique where it separates solutes according to their sizes

separating compound based primarily on their volatility

I. Adsorption is liquid-liquid chromatography

II. It is also a competition between the sample and the mobile phase for the absorptive sites

III. Its stationary phase is only nonpolar

chromatography procedure that uses pressure for fast separations, controlled temperature, in-line detectors and gradient elution techniques

basic component of HPLC:

- can be used to introduce sample into the path of the mobile phase that carries it into the column

What step in conventional electrophoresis wherein you will see the substance will move