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WorksheetsBACTE REVIEW
Total questions: 37
Worksheet time: 20mins
Which among the following rapidly catalyzes the breakdown of hydrogen peroxide to water and oxygen?
Catalase
Peroxidase
Oxidase
Amidase
Which of the following is coagulase positive?
S. saprophyticus
S. intermedius
S. epidermidis
S. aureus
What is the indicator used to detect the b-naphthylamide in PYR test?
p-aminobenzaldehyde
N,N-methylphenyldiamine
N,N-methylaminocinnamaldehyde
Alpha-naphthol
Which product of Hippurate hydrolysis is detected by the test?
Benzoic acid
Glycine
Glycerol
Hydrogen ions
Which of the following is describes the positive result for Oxidase test?
Intense Red Color
Deep purple/black
Yellow
Green Color
What substrate is hydrolyzes resulting to the formation of indole?
Indican
Tryptophan
Citrate
Carbohydrate
Which of the following is altered structurally by DNAse when complexed with DNA?
Methylene Blue
Toluidine blue
Methyl Green
Bromcresol Purple
What is the positive result for Urease test?
Pink
Blue
Red
Orange
Which of the following results is associated with oxidizers in O-F test?
Open and closed tubes changes color to yellow
Open tube shows changes in color at the upper portion of deep
Neither of the tube changes during incubation
Only the closed tube changes color
What is the indicator used for hydrogen sulfide production in TSIA?
Ferric ammonium sulfate
Ferrous Sulfate
Iron (II) Sulfide
Sodium thiosulfate
Which of the following organisms is/are Hydrogen sulfide producers?
Enterobacter spp.
Edwardsiella spp.
Salmonella spp.
Shigella spp.
Which of the following organism is matched with its MRVP results?
E. coli:
MR (-) VP (+)
K. pneumoniae: MR (-) VP (+)
Hafnia spp:
MR (+) VP (+)
S. marcescens: MR (+) VP (-)
Which fermentation product is detected by the MR test?
Acetoin
Gas
Acid
Carbohydrates
Which of the following is the decarboxylation product of lysine?
Citrulline
Cadaverine
Putrescine
Glutamine
What reagent is added to detect the product of phenylalanine deamination?
Ferric Chloride
Hydrochloric Acid
Potassium Hydroxide
Alpha-Naphthol
What does a red slant mean in LIA?
Decarboxylation
Deamination
Fermentatiopn
Hydrogen sulfide production
Which of the following organisms is capable of oxidative deamination?
K. pneumoniae
E. coli
P. vulgaris
Shigella spp.
What pH indicator is used in citrate utilization test?
Bromcresol purple
Phenol Red
Bromthymol blue
Methyl Red
Which of the following inhibits gram-positive bacteria?
Colistin
Vancomycin
Kanamycin
Bacitracin
Which of the following is resistant to novobiocin?
S. saprophyticus
S. epidermidis
Which of the following method is recommended by CLSI when performing Antimicrobial Susceptibility Testing?
Epsilometer Test
Broth Dilution Method
Agar Dilution Method
Disk Diffusion method
What is the approximate inoculum load is similar to the turbidity of 0.5 Mcfarland standard?
1.0 x 108 cells/mL
1.5 x 108 cells/mL
1.0 x 109 cells/mL
1.5 x 109 cells/mL
What acid is added to 1.75% w/v BaCl to make 0.5 McFarland Standard?
0.36N H2SO4
1N HCl
1% Acetic Acid
0.048M H2SO4
What method is used to prepare the inoculum suspension for S. aureus?
Log phase Method
Broth Method
Direct NSS Suspension
Agar Method
What is the standard depth of MHA and volume in a standard 100mm petri dish to perform AST?
4 mm: 25 mL
6 mm: 50 mL
4mm: 60 mL
6mm:30 mL
What inoculation technique is employed when inoculating MHA plates for AST?
Quadrant Streaking
Radiant Streaking
Continuous streaking
Singe Streaking
How many antibiotic disks can a 150mm petri plate accommodate?
4 - 5
6 - 8
9 - 11
up to 12
How should AST plates for Haemophilus species incubated?
Capnophilic incubation
Aerobic Incubation
Anaerobic Incubation
Cannot be cultivated
What component is incorporated to MHA to grow S. pneumoniae?
GC Agar Base
Bile Salts
1% NaCl
2 - 5% Horse Blood
What component is incorporated to MHA to grow MRSA?
GC Agar Base
Bile Salts
1% NaCl
2 - 5% Horse Blood
Select all tests that are classified as Single Enzyme Tests
Bacitracin Susceptibility Tests
Coagulase test
Citrate Utilization Test
Phenylalanine Deamination Test
Catalase Test
Select all tests that are classified as Single Substrate Utilization
Acetamide Utilization Test
Coagulase test
Citrate Utilization Test
Phenylalanine Deamination Test
Catalase Test
Select all tests that are classified as establishing inhibitory profiles.
Acetamide Utilization Test
Bacitracin Susceptibility test
Citrate Utilization Test
Phenylalanine Deamination Test
Novobiocin Susceptibility Test
Select all tests that are classified as Carbohydrate Utilization test
MRVP Test
Bacitracin Susceptibility test
O-F Test
Indole Test
Novobiocin Susceptibility Test
Select all tests that are classified as amino acid utilization.
Acetamide Utilization Test
Bacitracin Susceptibility test
Citrate Utilization Test
Phenylalanine Deamination Test
Novobiocin Susceptibility Test
Which among the following will falsely increase the zone of inhibition?
Dispensed 10 mL of MHA in 100 mm Petri Dish
Turbidity of suspension is cloudy
Antibiotic disks came in contact with water droplets
Dispensed 25 mL of MHA in 100mm Petri Dish
Antibiotic fell on petri dish cover
Which among the following will falsely decrease the zone of inhibition?
Acidic MHA on Tetracycline
E. coli in TSB tube was tightly sealed before incubation
Dispensed 80 mL of MHA in 150mm Petri Dish
Acidic MHA on Nitrofurantoin
Haemophilus species subjected to log phase growth
