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WorksheetsMicroorganisms
Total questions: 25
Worksheet time: 1hrs 15mins
To avoid false-positive staining in the demonstration of AFB, distilled water _____.
should never be used for the rinse steps
must be used before the primary dye/stain is applied
should never be used after the application of the carbon-fuchsin solution
should be used for the entire procedure
Upon observing a section stained with the Kinyoun AFB method, you notice that the counterstain methylene blue has covered or masked the organism being demonstrated you should:
cut another section and start over
don't do anything, submit to the pathologist
run the slide back to the beginning of the procedure
take the slide back to acid alcohol to remove methylene blue, wash with water and retain with counterstain
The most commonly used phenolic compound for reducing adsorbed silver to a visible metallic state in argyrophilic procedures, such as the Warthin Starry, is:
sodium thiosulfate
hydroquinone
sodium bisulfate
uranyl nitrate
From the following, what is the most likely cause of an AFB stained control that gives a negative result?
millipore water was used for the whole procedure
too much heat was used
non-coated slides were used
carnoy's was used to fix the tissue
In order for the Gram stain to work properly, it is important to treat the tissue with a mordant:
before the primary dye
after the 2nd dye is applied
after the 1st dye is applied
in the same solution as the primary dye
The control section containing both gram-positive and gram negative organisms shows only gram positive organisms when stained with the Brown & Hopps technique. This is most likely the result of:
insufficient time in the iodine solution
insufficient time in the Gallego solution
over differentiation with the Gallego solution
improper application of the crystal violet
Acid-fast organisms are seen on a section of lung tissue. The organisms appear to lie out of focus on the surface of the section. This is most likely the result of:
a section that is too thick
poor mounting technique
contamination from the water bath
the nature of the diseased tissue
Upon microscopic examination of gram positive tissue, you noticed that the organism has stained weakly and you also notice traces of mercury pigment. This is most likely the cause of:
the iodine solution step was skipped
a weak solution of crystal violet
too strong solution of basic fuchsin
formalin was left out of the gallego solution
Organisms found on the gastric mucosa of the stomach presumptively the cause of gastritis which are which of the following microorganisms and will be demonstrated with a silver technique:
helicobacter pylori
leptospira interrogans
treponema pallidum
borrelia burgdorferi
After you have performed the Fite's AFB technique for leprosy organisms, you notice that significant shrinkage of the tissue has occurred. This is most likely the cause of:
not using phenol crystals in the carbol-fuchsin solution
excessive blotting
not using a mixture of xylene-peanut oil during deparaffinization
too much heat
The slides exhibit a blackish precipitate after performing a steiner procedure for spirochetes. This is most likely the cause of:
too much heat
not using phenol crystals in the carbol-fuchsin solution
not using a mixture of xylene-peanut oil during deparaffinization
excessive blotting
What color do the microorganisms fluoresce when treated with the Auramine O Rhodamine B reagent?
greenish-red
bluish-red
reddish-yellow
reddish-green
The property of acid-fastness appears to be related to the presence of this substance within the organisms or within the cell walls of organism:
lipids
mucosubstances
polysaccharides
protein
Spirochetes are filamentous bacteria and are known to be:
argentophilic
rod shaped
self-reducing to visible silver
argyrophilic
The pathologist has ordered a Giemsa stain to differentiate a specific bacterium but upon microscopic examination, the pathologist notes that the Giemsa technique did not work properly. What should the histologist do?
inform the pathologist that the Giemsa stain is not used to identify a specific type of bacteria
perform a silver technique, an AFB, and a Gram stain
perform the Giemsa technique again using a new control slide
nothing, it is not the tech's business to question the pathologist
For the Brown-Hopps stain for bacteria, the second differentiator is:
acid alcohol
95% ETOH
Gallego solution
70% ETOH
From the following procedures, which will be best to identify helicobacter pylori?
Truant
Diff-Quik
Fite
Gram
The bacterium Legionella pneumophilia can be demonstrated using this staining method:
AFB
Steiner & Steiner
Gram
Grimelius
The color result for the Kinyoun AFB technique is bacteria are _____ and the background is _____.
yellow/black
black/bright red
bright red/blue
blue-black/
bright red
The purpose of iodine in Gram techniques for staining bacteria is to:
form a dye-lake with primary crystal violet stain
inactivate bacteria
decolorize crystal violet
make the cell walls of bacteria permeable
The choice of a decolorizer for the Ziehl-Neelson stain for AFB is:
aqueous citric acid solution
10% HCL acid in 70% ETOH
picric acid in 95% ETOH
1% HCL acid in 70% ETOH
The tissue section has been over-developed using the Warthin Starry technique for spirochetes, how can you best correct the problem?
cut another section and start over
treat with iodine and sodium thiosulfate, wash and restain
run the slide back to water and rinse with hot tap water for an hour
there is nothing that can be done once the reaction has occurred
Acid-fast techniques act on the condition in which the organism has:
to be excessively blotted for bacterial affinity
the ability to resist decolorization with a weak acid
phenolic crystal abberation
the capability for reducing argentaffin granules
The recommended fixative for tissue suspected of containing spirochetes:
10% NBF
carnoy's
zenker's
bouin's
Insoluble compounds - meaning a layer of crust to form on top of the tissue that will cause the technique to fail - are noted on a microscopic evaluation of a control section stained with the brown & hopps gram stain. The presence of these compounds are probably due to:
perform procedure on frozen sections only
inadequate fixation
omission of mordant during staining
the tissue sections were allowed to dry-out
