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Science and Technology of Food WebXam Review #2

Total questions: 70

Worksheet time: 53mins

Name
Class
Date
1.
What is the backbone of DNA made of?
a)
Glucose and Nitrogen
b)
Deoxyribose and Nitrogen
c)
Deoxyribose and Phosphate
d)
Deoxyribose and Sulfate
2.
What kind of bonds hold the DNA molecule together?
a)
Phosphate bonds and Hydrogen bonds
b)
Dipolar bonds and Hydrogen bonds
c)
Hydrogen bonds an Sulfate bonds
d)
Covalent bonds and Hydrogen bonds
3.
Which bases pair together in the DNA molecule?
a)
A-G and C-T
b)
A-T and C-G
c)
A-C and G-T
d)
A-U and G-C
4.
Which best describes an Okazaki fragment?
a)
A piece of single strand DNA that gets replicated into double strand DNA
b)
A small piece of RNA attached to the DNA strand to start replication.
c)
Complementary pieces of DNA that match to the lagging strand
d)
A DNA primer that matches the target DNA
5.
Which enzyme seals the fragments of DNA into a continuous strand?
a)
DNA Ligase
b)
DNA Polymerase
c)
Helicase
d)
Exonuclease
6.
Which of the following excises introns from the mRNA strand?
a)
tRNA
b)
Exons
c)
Spliceosome
d)
RNA Polymerase
7.
Which enzyme is responsible for elongating the mRNA strand?
a)
DNA polymerase
b)
RNA polymerase
c)
Promoter region
d)
Ribosome
8.
Which enzyme begins DNA replication with a small strand of RNA?
a)
Helicase
b)
Primase
c)
DNA Ligase
d)
DNA Polymerase
9.
The Gene Expert cartridge allows for which of the following?
a)
All PCR reagents are contained within one cartridge
b)
Mutiplex PCR for multiple targets
c)
PCR amplification of RNA viruses
d)
Nested PCR
10.
Which enzyme adds base pairs to elongate  single stranded DNA?
a)
Exonuclease
b)
DNA Ligase
c)
Primase
d)
DNA polymerase
11.
What is RFLP used for?
a)
Detecting PCR products
b)
Amplify nucleic acids
c)
Used for Eastern blot
d)
DNA fingerprinting
12.
Which step in PCR is responsible for hybridization?
a)
Annealing
b)
Initiation
c)
Denaturation
d)
Extension
13.
Why is Taq used in PCR?
a)
It is a specific DNA Primer
b)
It is a heat stable polymerase
c)
It is specific to the target probe
d)
It does not need a cofactor
14.
What is RT-PCR used for?
a)
To detect PCR products as they are produced.
b)
To quantify PCR products
c)
To detect a viral load
d)
To amplify RNA viruses
15.
Which of the following techniques allows for mutiplexing?
a)
Branched DNA
b)
Nested PCR
c)
Real time PCR
d)
Reverse Transcriptase PCR
16.
Which of the following results in an amino acid substitution?
a)
Deletion
b)
Insertion
c)
Nonsense
d)
Missense
17.
What is the major drawback of the BioFire Film Array?
a)
It can only detect one PCR target
b)
It cannot detect slowgrowing organisms
c)
It can only do one sample per hour
d)
It cannot detect resistance genes
18.
What advantage does the MALDI-TOF provide?
a)
It takes minutes instead of days for  identification of pathogens
b)
It can detect multiple targets
c)
It is inexpensive
d)
It can detect antibiotic resistance
19.
What testing principle is utilized by the MALDI-TOF?
a)
PCR
b)
RT-PCR
c)
Multiplex PCR
d)
Mass Spectrometry
20.
In gel electrophoresis, what separates the DNA fragments?
a)
They are separated by an electrical field
b)
They are separated by magnetic fields
c)
They are separated by binding to the gel
d)
They are separated by the electrical charge of the gel
21.

The rationale for implementing an overall quality management system in the laboratory is to

a)

prevent any possibility of testing error

b)

differentiate between qualitative and quantitative methods

c)

prevent potential errors in the laboratory’s path of workflow

d)

help to ensure that testing performed by the laboratory is accurate and reliable

22.

Ensuring that a product or service meets minimum standards often through testing a sample of products once they have been made.

a)

Quality Management

b)

Quality Assurance

c)

Quality Control

d)

Quality Committee

23.

What does ISO stand for?

a)

In Search Of

b)

International Standardization Organization

c)

International Organization for Standardization

d)

International Standardization and Organization

24.

SOP is . . . .

a)

Standard Operating Procedure

b)

Standard Operational Procedure

c)

Standard Operation Procedure

d)

Standard Operate Procedure

e)

Standard Operator Procedure

25.

When in doubt...

a)

eat it up fast.

b)

throw it out.

c)

give it to a friend.

26.

Which are examples of food to food cross-contamination?

a)

egg spills on apple

b)

sneeze on apple

c)

apple drops into cheese

d)

knife cuts through cheese and apple sandwich

27.

Which are examples of food to self cross-contamination?

a)

egg spills on apple

b)

sneeze on apple

c)

apple drops into cheese

d)

onion juice gets into your eye

28.

When do you wash your hands?

a)

before cooking

b)

before touching a different group of foods

c)

after your cough or sneeze

d)

before you dry the dishes and put them away

29.

What are the three domains of life?

a)

Animalia, Plantae, Fungi

b)

Archaea, Bacteria, Eukarya

c)

Kingdom, Phylum, Class

d)

Mammalia, Reptilia, Aves

30.
Which of the following statements are true?
a)
All microorganisms are bad
b)
All microorganisms are good
c)
Some microorganisms are harmful, while some can be helpful
d)
Microorganisms are too small to do anything to anyone
31.

Culture media: nutrient material prepared for bacterial growth in the laboratory. Which one is not a requirement for culture media?

a)

must be sterile

b)

must contain appropriate nutrient

c)

must be incubated at appropriate temperature and pH

d)

It must be incubated in dark environment

32.

Liquid media is called

a)

agar

b)

broth

33.

Which is essential for a cell's growth?

a)

Aseptic lab

b)

Growth media

c)

Skilled technician

d)

All the above

34.

There are over _____ microorganisms on and in the human body.

a)

5,000

b)

1 Trillion

c)

100 Billion

d)

100 Trillion

35.

How much does the bacteria living in and on the human body weigh?

a)

10 to 12 pounds

b)

0 to 2 pounds

c)

5-10 pounds

d)

2 to 5 pounds

36.

What are the advantages of having microorganisms living on and in our body?

a)

They help break down the food we eat and they help protect us from infection by other harmful microorganisms

b)

They help us grow and develop our behavior and traits

c)

They help us by cleaning our bodies of dirt, debris, and toxic chemicals

d)

They help us by playing an important role in developing our senses

37.

If a bacterial culture has a doubling time of 30 minutes, and after 2.5 hours there are 160 cells, how many cells were there to begin with? 

a)

5

b)

10

c)

15

d)

20

38.

In an experiment to find out how the temperature affects the bounce height of a ball, the dependent variable is ...

a)
temperature
b)
bounce height
c)
the thermometer
d)
the ruler
39.
Should experiments be repeated over and over to see if the results are the same each time?
a)
yes
b)
no
40.

The one variable in an experiment that is purposely changed and tested by the scientist is the________________.

a)

dependent variable

b)

independent variable

c)

data

d)

control group

41.

Research Question: Does the type of gasoline put in a car affect how fast the car can drive.

What is the independent variable?

a)

Type of gasoline

b)

which car the gas is put into

c)

how fast the car can drive

d)

how much gas is put into the car

42.
If I study for my scientific method quiz, then I will have a better test score. What is the dependent variable?
a)
Scientific Method
b)
Studying
c)
Independent variable
d)
Test score
43.
What is the independent variable?
a)
cost of mowing
b)
Moe's Mowing Service
c)
$18
d)
Mowing Time
44.

In a line graph you will find the independent variable on .... axis and the dependent variable on .... axis

a)

x , y

b)

y, x

c)

they can be switched

45.
A testable prediction about a scientific investigation is a(n)
a)
Question
b)
Observation
c)
Hypothesis
d)
Experiment
46.

How many independent variables should you have in an experiment?

a)

two

b)

as many as possible

c)

four

d)

one

47.

This part of the experiment serves as a basis of comparison; the group that does not receive the independent variable.

a)

constant

b)

control group

c)

experimental group

d)

hypothesis

48.

An experiment is performed on plants to see how different liquids affect plant growth. Each plant in the experiment is given a different liquid: water, apple juice, or milk. What should be kept constant? 

a)
type of plant, flower pot,  soil, and amount of sunlight
b)
water, apple juice, milk
c)
only the amount of sunlight
d)
type of plant only
49.
A series of steps used by scientists to solve a problem or answer a question. 
a)
scientific method
b)
recipe
c)
data collection
d)
metric system
50.

This is the group that is affected by the variable or the group that is changed.

a)

Control Group

b)

Experimental Group

c)

Changed Group

d)

Cool Group

51.
This is the group that we will compare our experimental group to. This group stays "normal."
a)
Control Group
b)
Experimental Group
c)
 Normal Group
d)
Weird Group
52.

If I floss more, then I will have fewer cavities. What is the independent variable?

a)

Number of cavities

b)

Amount of flossing

c)

Type of floss used

d)

Amount of brushing

53.

What is the independent variable on this graph?

a)

Number of push ups

b)

Days

54.

During which month did Sam have a weight of 73 Kg?

a)

January

b)

February

c)

May

d)

March

55.
What is a constant? 
a)
the normal situation
b)
the thing we measure in the end
c)
the thing we are testing
d)
the thing we keep the same for every part of the experiment
56.
What is the dependent variable?
a)
Grade
b)
Number of Students
57.

What is the independent variable?

a)

Months

b)

Temperature

c)

Highs

d)

Lows

58.

PPE stands for what?

a)

Personnal Protection Equipment

b)

Personnal Protective Equipment

c)

Personels Protective Equipment

d)

Parental Protection Equilibrium

59.

What kind of hazard is show below.

a)

Noise hazard

b)

Chemical hazard

c)

Biological hazard

d)

Ergonomic hazard

60.

The picture shows what kind of PPE.

a)

Disposable lab gown

b)

Face mask

c)

Goggles

d)

Gloves

61.

The picture shown what kind of hazard.

a)

Electrical hazard

b)

Biochemical hazard

c)

Chemical hazard

d)

Ergonomic hazard

62.

The picture shown below what kind of hazard

a)

Biological hazard

b)

Physical hazard

c)

Chemical hazard

d)

Manual handling hazard

63.

What is the use or importance of wearing correct PPE while conducting nail care?

4 lines
64.

The picture shown what kind of PPE equipment

a)

Lab gown

b)

Face shield

c)

Gloves

d)

Face mask

65.

This equipment will cover your entire hands from contacting different chemicals while handling your clients

a)

Gloves

b)

Goggles

c)

Lab gown

d)

None of the above

66.

The picture shown what kind of hazard

a)

Ergonomic hazard

b)

Chemical hazard

c)

Physical hazard

d)

Manual handling hazard

67.

Give at least 1 advantage of using PPE.

4 lines
68.

What is meant by 'PPE'?

a)

personal protective equipment

b)

person to person equivalent

c)

personal protective evacuation

69.

Which of the following is NOT an example of personal protective equipment?

a)

Stair Rail

b)

Safety Glasses

c)

Hard Hat

d)

Gloves

70.

Workplace safety is the responsibility of __________________.

a)

Employees

b)

Employers

c)

Everyone

d)

Supervisors

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