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Recombinant Bacteria and Transformation

Total questions: 45

Worksheet time: 26mins

Name
Class
Date
1.

Which of the following is NOT a tool used in genetic engineering?

a)

plasmid

b)

restriction enzyme

c)

DNA helicase

d)

DNA ligase

2.

Which restriction enzyme listed below was isolated from E. coli bacteria?

a)

EcoRI

b)

HindIII

c)

HaeII

d)

SmaI

3.

During insertion step,

a)

The recombinant plasmid is inserted into a host cell

b)

The gene of interest is inserted into a plasmid

c)

The plasmid is inserted into the gene of interest

4.
Which protein is responsible for allowing the bacteria to grow in the presence of ampicillin?
a)
GFP
b)
araC
c)
Beta Lactamase
d)
Restriction Enzyme
5.
What is the name of the plasmid we used for lab?
a)
GFP
b)
bla
c)
araC
d)
pGLO
6.
Which gene is responsible for making the bacteria resistant to ampicillin?
a)

Antibiotic resistance gene

b)
pGLO
c)
araC
d)
GFP
7.
 What was used to cut the plasmid to enable us to insert the desired gene?
a)
ligase
b)
restriction enzymes
c)
GFP
d)
pGLO
8.
What did we use to create temperature differential to sweep plasmid inside
a)
heat shock
b)
ice
c)
arabinose
d)
CaCl2
9.
Which plate had bacterial growth and bacterial GLOWth?
a)
LB
b)
LB/Amp
c)
LB/Amp/Ara
d)
All
10.
What is used to ensure the bacteria transformed with the gene for antiobiotic resistance as only the transformed bacteria will grow on this medium?
a)
ampicillan
b)
arabinose
c)
LB
d)
GFP
11.
Which gene is responsible for the bacteria glowing?
a)
bla
b)
araC
c)
GFP
d)
pGLO
12.
Which gene is responsible for "turning on" the glow?
a)
bla
b)
ARAc
c)
GFP
d)
LB
13.
What abbreviation represents the sugar molecule needed to "turn on" the green fluorescent protein gene?
a)
LB
b)
AMP
c)
ARA
d)
+pGlo
14.
Which gene WAS NOT included in the +pGlo plasmid?
a)
antibiotic resistance gene
b)
green fluorescent protein gene
c)
hemophilia gene
15.
Making changes in the DNA of an organism is
a)
selective breeding
b)
artificial selection
c)
genetic engineering
d)
genetic transformation
16.
What is the circular piece of DNA found in bacteria called?
a)
homologous chromosome
b)
sister chromatid
c)
plasmid
d)
arabinose
17.

Process of gene transfer where a virus moves genetic material from one bacteria to another

a)

conjugation

b)

transdution

c)

transformation

18.

What is the blue circle toward the bottom of the prokaryote called?

(a)  

19.

What is the red stringy thing in the middle called?

(a)  

20.

Process of gene transfer where a virus die/bursts & another bacteria takes in the free-floating plasmids

a)

conjugation

b)

transduction

c)

transformation

21.

The process gene transfer where bacteria connect pili to exchange plasmids

a)

conjugation

b)

transformation

c)

transduction

22.
Which of the following accurately describe the steps of recombinant DNA technology using bacteria?
a)
plasmid & genes are cut --> cut DNA joined with ligase to make rDNA plasmids --> rDNA plasmids are inserted into the bacteria --> bacteria & rDNA plasmid multiply --> bacteria are broken open & protein is collected
b)
cut DNA joined with ligase to make rDNA plasmids --> rDNA plasmids are inserted into the bacteria --> plasmid & genes are cut -->bacteria & rDNA plasmid multiply --> bacteria are broken open & protein is collected
c)
plasmid & genes are cut -->bacteria & rDNA plasmid multiply --> bacteria are broken open & protein is collected-->cut DNA joined with ligase to make rDNA plasmids --> rDNA plasmids are inserted into the bacteria
d)
cut DNA joined with ligase to make rDNA plasmids --> rDNA plasmids are inserted into the bacteria --> bacteria & rDNA plasmid multiply 
23.
Which gene is responsible for making the bacteria resistant to ampicillin?
a)

bla (beta-lactamase)

b)
pGLO
c)
araC
d)
GFP
24.

What does it mean for cells to be competent

a)

They are made to be 'smart' through genetic engineering

b)

Their cell walls are altered to allow DNA to pass more easily

c)

They contain regular DNA and plasmid DNA

d)

They contain a gene for resistance to ampicillin

25.

What process increases the competency of cells?

a)

calcium chloride and an ice bath

b)

calcium chloride and heat shock

c)

Luria broth (LB) and an ice bath

d)

Luria broth (LB) and heat shock

26.

Which is the correct sequence for the process of genetic transformation?

a)

bacterial cells transferred to cold CaCl2 , plasma with foreign DNA added bacteria , cells heat shocked at 42oC , cells spread on agar petri dish , bacteria given time to grow on petri dish

b)

bacterial cells transferred to cold CaCl2 , cells heat shocked at 42oC , plasma with foreign DNA added bacteria , bacteria given time to grow on petri dish , cells spread on agar petri dish

c)

plasma with foreign DNA added bacteria , bacterial cells transferred to cold CaCl2 , cells spread on agar petri dish , bacteria given time to grow on petri dish , cells heat shocked at 42oC

27.

Which one of these agar plates contains bacteria most like the original bacterial culture

a)

+pGLO LB/amp

b)

+pGLO LB/amp/ara

c)

-pGLO LB/amp

d)

-pGLO LB

28.

Which one of these agar plates would there be no bacteria growth?

a)

+pGLO LB/amp

b)

+pGLO LB/amp/ara

c)

-pGLO LB/amp

d)

-pGLO LB

29.

Which image is a plasmid?

a)
b)
c)
d)
30.

The plasmids scientists use in a lab are MOST COMMONLY found in

a)

plants

b)

animals

c)

archaea

d)

bacteria

31.

All plasmids have an origin of replication which is necessary for

a)

DNA to be duplicated

b)

DNA to be transcribed into RNA

c)

DNA to be inserted easily

d)

DNA to be cut open

32.

All plasmids have a promoter region which is necessary for

a)

DNA to be duplicated

b)

DNA to be transcribed into RNA

c)

DNA to be inserted easily

d)

DNA to be cut open

33.

When bacteria is able to take up DNA, it is considered

a)

permeable

b)

semi-permeable

c)

competent

d)

curated

34.

This image is representing a successful bacterial transformation

a)

True

b)

False

35.

Why are cells incubated at 42o C?

a)

At 42o C, bacterial cells begin releasing DNA for other cells to take up

b)

This is a good recovery temperature after heat shock

c)

At 42o C, the membranes of bacterial cells begin to degrade, creating pores through which DNA can enter the cell

36.

Ampicillin (antibiotic) is used in the plates to

a)

isolate and select for only the bacteria that have been successfully transformed

b)

give bacteria energy to grow

c)

act as a trigger to turn on the expression of the gene of interest

d)

give the bacteria a new trait (such as fluorescence or insulin production)

37.

Arabinose is used in the plates to

a)

isolate and select for only the bacteria that have been successfully transformed

b)

give bacteria energy to grow

c)

act as a trigger to turn on the expression of the gene of interest

d)

give the bacteria a new trait (such as fluorescence or insulin production)

38.

Describe the source of the fluorescence.

a)

from jellyfish

b)

from fireflies

c)

from a plant

d)

from the pGFP encoded by the plasmid

39.

Cells that fluoresce under UV light prove successful transformation and are on the plate

a)

without DNA and with ampicillin

b)

with DNA and ampicillin

c)

with DNA and without ampicillin

d)

without DNA or ampicillin

40.

In a plate with DNA and ampicillin plated with pGFP cells what would the results demonstrate?

a)

transformation of cells resistant to ampicillin

b)

transformation of cells not resistant to ampicillin

c)

transformation of no cells

d)

transformation of cells that are both sensitive and resistant to ampicillin

41.

In a plate with DNA but without ampicillin plated with GFP cells what would the result demonstrate?

a)

host bacterial cells are viable in the absence of ampicillin

b)

no growth

c)

untransformed and transformed cells are viable in the absence of ampicillin

d)

transformation of cells resistant to ampicillin

42.

In the plate with no DNA but with ampicillin what would the result be?

a)

white colonies

b)

no growth

c)

smeared layer of cells

d)

flourescent cells

43.

In a plate with no DNA or ampicillin, there are no fluorescent cells visible, what does this demonstrate?

a)

host bacterial cells are viable in the absence of ampicillin

b)

host bacterial cells are sensitive to ampicillin

c)

host bacterial cells are untransformed

d)

host bacterial cells are resistant to ampicillin

44.

What is pGFP?

a)

A plasmid

b)

A chromosome

c)

A protein

45.

The success of bacterial transformation is determined by:

a)

Transformation efficiency

b)

transformation success rate

c)

standard deviation

d)

gene expression ratio

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